Draft genome sequence of Pseudomonas extremaustralis strain USBA-GBX 515 isolated from Superparamo soil samples in Colombian Andes

López, Gina; Díaz‐Cárdenas, Carolina; Shapiro, Nicole; Woyke, Tanja; Kyrpides, Nikos C.; Alzate, J. David; González, Laura; Restrepo, Silvia; Baena, Sandra

doi:10.1186/s40793-017-0292-9

Cited by 10 publications

(7 citation statements)

References 66 publications

(71 reference statements)

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“…It is also, as far as we know, the first time that a member of this species has been isolated from sewage sludge. It has previously been isolated from Antarctic ponds [ 44 ], soil samples [ 46 ] and permafrost sediments [ 47 ], and it has been detected in activated sludge by Ji et al [ 48 ] using Illumina Miseq sequencing, yet not isolated.…”

Section: Resultsmentioning

confidence: 99%

Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation

et al. 2023

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Paracetamol is one of the most used pharmaceuticals worldwide, but due to its widespread use it is detected in various environmental matrices, such as surface and ground waters, sediments, soils or even plants, where it is introduced mainly from the discharge of wastewater and the use of sewage sludge as fertilizer in agriculture. Its accumulation in certain organisms can induce reproductive, neurotoxic or endocrine disorders, being therefore considered an emerging pollutant. This study reports on the isolation, from sewage sludge produced in wastewater treatment plants (WWTPs), of bacterial strains capable of degrading paracetamol. Up to 17 bacterial strains were isolated, but only two of them, identified as Pseudomonas stutzeri CSW02 and Pseudomonas extremaustralis CSW01, were able to degrade very high concentrations of paracetamol in solution as a sole carbon and energy source, and none of them had been previously described as paracetamol degraders. These bacteria showed the ability to degrade up to 500 mg L−1 of paracetamol in only 6 and 4 h, respectively, much quicker than any other paracetamol-degrader strain described in the literature. The two main paracetamol metabolites, 4-aminophenol and hydroquinone, which present high toxicity, were detected during the degradation process, although they disappeared very quickly for paracetamol concentrations up to 500 mg L−1. The IC50 of paracetamol for the growth of these two isolates was also calculated, indicating that P. extremaustralis CSW01 was more tolerant than S. stutzeri CSW02 to high concentrations of paracetamol and/or its metabolites in solution, and this is the reason for the much lower paracetamol degradation by S. stutzeri CSW02 at 2000–3000 mg L−1. These findings indicate that both bacteria are very promising candidates for their use in paracetamol bioremediation in water and sewage sludge.

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Section: Resultsmentioning

confidence: 99%

Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation

et al. 2023

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“…extremaustralis 14‐3b possess characteristics related to plant growth promotion such as the capability of phosphate solubilization, indole acetic acid production and plant interaction (Ibarra, 2017). In addition, several studies have reported the finding of this species associated with plants in different environments as well as its plant growth promotion traits (Gasser et al ., 2009; Kudoyarova et al ., 2017; López et al ., 2017; Hong et al ., 2018; Arkhipova et al ., 2019). Thus, the expression of T3SS in P .…”

Section: Resultsmentioning

confidence: 99%

Small RNAs in the Antarctic bacterium Pseudomonas extremaustralis responsive to oxygen availability and oxidative stress

Venero

Matera

Vogel

et al. 2022

Environ Microbiol Rep

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SummaryBacterial small non‐coding RNAs (sRNAs) play key roles as genetic regulators, mediating in the adaptability to changing environmental conditions and stress responses. In this work, we analysed putative sRNAs identified by RNA‐seq experiments in different aeration conditions in the extremophile bacterium P. extremaustralis. These analyses allowed the identification of 177 putative sRNAs under aerobiosis (A), microaerobiosis (M) and microaerobiosis after H2O2 exposure (m‐OS). The size and transcription profile of eight sRNAs with differential expression were verified by Northern blot. sRNA40, with unknown function but conserved in other Pseudomonas species, was selected to perform overexpression experiments followed by RNA‐seq analysis. The overexpression of sRNA40 in P. extremaustralis resulted in significant expression changes of 19 genes with 14 differentially upregulated and five downregulated. Among the upregulated genes, eight transcripts corresponded to components of secretion systems, such as gspH, gspK, and gspM, belonging to the Type II secretion system, and rspO and rspP from Type III secretion system. Our results showed a novel sRNA which expression was triggered by low oxygen levels, and whose overexpression was associated with upregulation of selected components of protein secretion systems.

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“…The vent fluids from both Big Strýtan and Arnarnesstrýtan contain an abundance of Flavobacterium , Pseudoalteromonas , Psychrobacter , Acinetobacter , and Pseudomonas species ( Tables 2 , 3 and Supplementary Table 3 ). Type strain members of these genera are all aerobic, heterotrophic bacteria ( Akagawa-Matsushita et al, 1992 ; Bozal et al, 2003 ; Van Trappen et al, 2004 , 2005 ; Chaudhary et al, 2019 ), with the exception of Pseudomonas extremaustralis , which is capable of nitrate-reduction ( López et al, 2009 , 2017 ). Interestingly, almost all of the species detected exclusively in vent fluids are also found in Arctic and Antarctic samples ( Bozal et al, 2003 ; Van Trappen et al, 2004 , 2005 ; López et al, 2009 ; Chaudhary et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland

et al. 2022

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Strýtan Hydrothermal Field (SHF) is a submarine system located in Eyjafördur in northern Iceland composed of two main vents: Big Strýtan and Arnarnesstrýtan. The vents are shallow, ranging from 16 to 70 m water depth, and vent high pH (up to 10.2), moderate temperature (Tmax ∼70°C), anoxic, fresh fluids elevated in dissolved silica, with slightly elevated concentrations of hydrogen and methane. In contrast to other alkaline hydrothermal vents, SHF is unique because it is hosted in basalt and therefore the high pH is not created by serpentinization. While previous studies have assessed the geology and geochemistry of this site, the microbial diversity of SHF has not been explored in detail. Here we present a microbial diversity survey of the actively venting fluids and chimneys from Big Strýtan and Arnarnesstrýtan, using 16S rRNA gene amplicon sequencing. Community members from the vent fluids are mostly aerobic heterotrophic bacteria; however, within the chimneys oxic, low oxygen, and anoxic habitats could be distinguished, where taxa putatively capable of acetogenesis, sulfur-cycling, and hydrogen metabolism were observed. Very few archaea were observed in the samples. The inhabitants of SHF are more similar to terrestrial hot spring samples than other marine sites. It has been hypothesized that life on Earth (and elsewhere in the solar system) could have originated in an alkaline hydrothermal system, however all other studied alkaline submarine hydrothermal systems to date are fueled by serpentinization. SHF adds to our understandings of hydrothermal vents in relationship to microbial diversity, evolution, and possibly the origin of life.

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Draft genome sequence of Pseudomonas extremaustralis strain USBA-GBX 515 isolated from Superparamo soil samples in Colombian Andes

Cited by 10 publications

References 66 publications

Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation

Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation

Small RNAs in the Antarctic bacterium Pseudomonas extremaustralis responsive to oxygen availability and oxidative stress

Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland

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