Draft Genome Sequence of Pasteurella multocida A:3 Strain 671/90

Lainson, F. A.; Dagleish, Mark P.; Fontaine, Michaël C.; Bayne, C.W.; Hodgson, John

doi:10.1128/genomea.00803-13

Cited by 7 publications

(6 citation statements)

References 7 publications

(7 reference statements)

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“…On the other hand occurrence of the factors in apparently healthy lungs could possibly indicate early infection or contained infection which couldn't lead to disease. It was previously reported that this facultative anaerobic bacterium is commonly found in clinically healthy calves (Lainson et al, 2013 ).…”

Section: Discussionmentioning

confidence: 89%

Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran

2014

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A total of 30 Pasteurella multocida strains isolated from 333 pneumonic and apparently health slaughter cattle were examined for capsule biosynthesis genes and 23 virulence-associated genes by polymerase chain reaction (PCR). The disc diffusion technique was used to determine antimicrobial resistance profiles among the isolates. Of the isolates, 23 belonged to capsular type A, 5 to capsular type D and two isolates were untypeable. The distribution of the capsular types in pneumonic lungs and in apparently health lungs was statistically similar. All virulence genes tested were detected among the isolates derived from pneumonic lungs; whereas isolates derived from apparently health lungs carried 16 of the 23 genes. The frequently detected genes among isolates from pneumonic lungs were exbD, hgbA, hgbB, ompA, ompH, oma87, and sodC; whereas tadD, toxA, and pmHAS genes occurred less frequently. Most of the adhesins and superoxide dismutases; and all of the iron acquisition and protectin proteins occurred at significantly (p ≤ 0.05) higher frequencies in isolates from pneumonic lungs. Isolates from apparently healthy lungs didn't carry the following genes; hsf-1, hsf-2, tadD, toxA, nanB, nanH, and pmHAS. One adhesion (hsf-1) and two iron acquisition (exbD and tonB) genes occurred at significantly (p ≤ 0.05) higher frequencies among capA isolates. All the P. multocida isolates were susceptible to ciprofloxacin, co-trimoxazole, doxycycline, enrofloxacin, nitrofurantoin, and tetracyclines. Different proportions of the isolates were however resistant to ampicillin, amoxicillin, erythromycin, lincomycin, penicillin, rifampin, streptomycin, and florfenicol. Our results reveal presence of virulence factors (VFs) in P. multocida strains isolated from symptomatic and asymptomatic bovids. A higher frequency of the factors among isolates from symptomatic study animals may suggest their role in pathogenesis of P. multocida-associated bovine respiratory disease (BRD). The results further reveal occurrence of antimicrobial resistance among some isolates. Control strategies for this pathogen, which could include development of an effective vaccine, are warranted so as to mitigate the social and economic consequences attributable to natural infections with this bacterium.

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Section: Discussionmentioning

confidence: 89%

Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran

2014

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“…Therefore, our results are in agreement with those previously documents (Adler et al, 1999 ; Wilson and Ho, 2013 ). It has been noted that P. multocida Carter: Heddleston serotype B: 2 strains are commonly responsible for bovine haemorrhagic septicaemia while serovar A: 3 strains are associated with bovine respiratory diseases (Welsh et al, 2004 ; Dey et al, 2007 ; Shivachandra et al, 2011 ; Lainson et al, 2013 ; Wilson and Ho, 2013 ). Both haemorrhagic septicaemia and respiratory diseases including pneumonia are the common infections associated with P. multocida in bovine species (bovine pasteurellosis) (Wilson and Ho, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

et al. 2018

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Pasteurella multocida is a leading cause of respiratory diseases in many host species. To understand the genetic characteristics of P. multocida strains isolated from different host species, we sequenced the genomic DNA of P. multocida isolated from pigs and analyzed the genetic characteristics of strains from avian species, bovine species, pigs, and rabbits using whole genome sequence (WGS) data. Our results found that a capsular: lipopolysaccharide (LPS): multilocus sequence typing (MLST) genotype A: L1: ST129 (43.75%) was predominant in avian P. multocida; while genotypes B: L2: ST122 (60.00%) and A: L3: ST79 (30.00%) were predominate in bovine P. multocida; genotype D: L6: ST50 (37.50%) in porcine P. multocida; and genotype A: L3: ST9 (76.47%) in rabbit P. multocida. Comparative genomic analysis of P. multocida from different host species found that there are no genes in the P. multocida genome that are specific to any type of host. Phylogenetic analysis using either whole-genome single nucleotide polymorphisms (SNPs) or the set of SNPs present in all single-copy core genes across genomes showed that P. multocida strains with the same LPS genotype and MLST genotype were clustered together, suggesting the combining both the LPS and MLST typing schemes better explained the topology seen in the P. multocida phylogeny.

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“…Isolate 619/90 was passaged through another calf to confirm its virulence and generated a new pathogenic isolate, 671/90 ( 3 ). Here, we report the genome sequences of strains 618/90 and 619/90, four of the five field isolates (one was not available), and resequencing of 671/90 ( 4 ), using Illumina short-read and Oxford Nanopore Technologies long-read sequencing to produce single-contig de novo genome assemblies.…”

Section: Announcementmentioning

confidence: 99%

De Novo Whole-Genome Sequencing and Annotation of Pathogenic Bovine Pasteurella multocida Type A:3 Strains

Livingstone

Aitchison

Dagleish

et al. 2020

Microbiol Resour Announc

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Draft Genome Sequence of Pasteurella multocida A:3 Strain 671/90

Cited by 7 publications

References 7 publications

Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran

Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

De Novo Whole-Genome Sequencing and Annotation of Pathogenic Bovine Pasteurella multocida Type A:3 Strains

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