Draft Genome Sequence of Parageobacillus thermoglucosidasius Strain TG4, a Hydrogenogenic Carboxydotrophic Bacterium Isolated from a Marine Sediment

Inoue, Masao; Tanimura, Ayumi; Ogami, Yusuke; Hino, Taiki; Okunishi, Suguru; Maeda, Hiroto; Yoshida, Takashi; Sako, Yoshihiko

doi:10.1128/mra.01666-18

Cited by 16 publications

(10 citation statements)

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“…The type strain DSM 2542 T is capable of growing under a mixture of both CO and O 2 by initially consuming O 2 via aerobic respiration, followed by CO by WGSR (Mohr et al, 2018a;2018b). Another strain, TG4, is capable of growing even under a 100% CO atmosphere (Inoue et al, 2019b).…”

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confidence: 99%

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Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>

et al. 2020

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The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energyconverting hydrogenase (CODH/ECH) in the thermophilic facultative anaerobe, Parageobacillus thermoglucosidasius. We initially compared the transformation efficiency of two strains, NBRC 107763 T and TG4. We then disrupted CODH, ECH, and both enzymes in NBRC 107763 T. The characterization of growth in all three disruptants under 100% CO demonstrated that both enzymes were essential for CO-dependent growth with hydrogen production in P. thermoglucosidasius. The present results will become a platform for the further metabolic engineering of this organism.

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confidence: 99%

“…The strains NBRC 107763 T (=DSM 2542 T ) (Suzuki et al, 1983) and TG4 (Inoue et al, 2019b) were used in the present study. Both strains were routinely cultured under Article ME20101 .…”

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Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>

et al. 2020

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“…In our study, a cellulase producing thermophilic bacterium, Parageobacillus thermoglucosidasius NBCB1 was isolated from a thermophilic bacterial consortium bred from vermicompost using RS as substrate. P.thermoglucosidasius has earlier been isolated from soil [32], marine environment [33], compost [34], wood wastes [35] and hot springs [36]. However, there are limited reports on the production of enzymes by the organism.…”

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confidence: 99%

Valorization of Rice Straw for Optimization of Production of Thermotolerant Cellulase by Parageobacillus Thermoglucosidasius NBCB1 Under Submerged Fermentation

Basak

Murmu

Gavande

et al. 2022

Preprint

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Rice straw can be a substrate for production of cellulolytic enzymes, a vital step in economical deconstruction of cellulosic biomass in industry. We herein report the optimization of cellulase production by the thermotolerant Parageobacillus thermoglucosidasius NBCB1, in rice straw based submerged fermentation. Optimization of process parameters by One Factor At a Time (OFAT) suggested Day 3, medium pH 5.5, 1% (v/v) sorbitol, 1% (v/v) peptone and a cultivation temperature of 60°C as the optimal conditions for cellulase production. Further optimization of effective variables: incubation period, medium pH, sorbitol and peptone concentrations, by Response Surface Methodology (RSM) and Artificial Neural Networking (ANN) improved cellulase yield to 3.106 ± 0.005EU and 3.488 ± 0.004 EU/mg protein, respectively, which were closer to model diagnosed values. OFAT, RSM and ANN improved cellulase production by ~ 6.66, ~ 9.34 and ~ 10 folds, respectively, compared with enzyme production in unoptimized medium, indicating the later two being equally effective in the maximization of cellulase production. Purified cellulase (MW 31 kDa) with pH and temperature optima of 5.5 and 60°C, respectively, retained 100% activity upon pre-incubation at 60°C indicating it’s thermostability. It also showed halotolerance and heavy metal tolerance properties. Due to it’s versatile nature, the cellulase produced by P. thermoglucosidasius NBCB1 could be suitable for industrial degradation of agroresidues.

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“…Genomic DNA extraction, library preparation, sequencing, and genome assembly were carried out as described previously ( 4 , 5 ). Genomic DNA was extracted using the DNeasy blood and tissue kit (Qiagen, Hilden, Germany); then, a DNA library was prepared using the Nextera mate pair library preparation kit (Illumina, San Diego, CA).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequence of Thermanaeromonas sp. Strain C210, Isolated in the Presence of Carbon Monoxide

Inoue

Tanimura

Fukuyama

et al. 2020

Microbiol Resour Announc

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Draft Genome Sequence of Parageobacillus thermoglucosidasius Strain TG4, a Hydrogenogenic Carboxydotrophic Bacterium Isolated from a Marine Sediment

Abstract: Parageobacillus thermoglucosidasius possesses biotechnological potential for fuel generation. Here, we report the draft genome sequence of P. thermoglucosidasius strain TG4, which was first isolated from a marine sediment.

Cited by 16 publications

References 19 publications

Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>

Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>

Valorization of Rice Straw for Optimization of Production of Thermotolerant Cellulase by Parageobacillus Thermoglucosidasius NBCB1 Under Submerged Fermentation

Draft Genome Sequence of Thermanaeromonas sp. Strain C210, Isolated in the Presence of Carbon Monoxide

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