Draft Genome Sequence of Multidrug-Resistant Strain
            <i>Citrobacter portucalensis</i>
            MBTC-1222, Isolated from Uziza (Piper guineense) Leaves in Nigeria

Igbinosa, Etinosa O.; Rathje, Jana; Habermann, Diana; Brinks, Erik; Cho, Gyu-Sung; Franz, Charles M. A. P.

doi:10.1128/genomea.00123-18

Cited by 12 publications

(12 citation statements)

References 9 publications

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“…Due to the high similarity in the genomes (99%), it is possible that A. hydrophila RIT668 shares this potential for broad metabolic capability, virulence and the ability to infect multiple hosts, including humans. The closest relative of RIT669 is a C. portucalensis strain; C. portucalensis strains can be multidrug resistant and some may be highly resistant livestock-origin pathogens or "superbugs" [135,136]. This raises the possibility that RIT669 may also be multidrug resistant.…”

Section: Discussionmentioning

confidence: 99%

Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria, Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669, Isolated from Endangered Turtles (Clemmys guttata)

et al. 2020

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Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669 were isolated from endangered spotted turtles (Clemmys guttata). Whole-genome sequencing, annotation and phylogenetic analyses of the genomes revealed that the closest relative of RIT668 is A. hydrophila ATCC 7966 and Citrobacter portucalensis A60 for RIT669. Resistome analysis showed that A. hydrophila and C. freundii harbor six and 19 different antibiotic resistance genes, respectively. Both bacteria colonize polyethylene and polypropylene, which are common plastics, found in the environment and are used to fabricate medical devices. The expression of six biofilm-related genes—biofilm peroxide resistance protein (bsmA), biofilm formation regulatory protein subunit R (bssR), biofilm formation regulatory protein subunit S (bssS), biofilm formation regulator (hmsP), toxin-antitoxin biofilm protein (tabA) and transcriptional activator of curli operon (csgD)—and two virulence factors—Vi antigen-related gene (viaB) and Shiga-like toxin (slt-II)—was investigated by RT-PCR. A. hydrophila displayed a >2-fold increase in slt-II expression in cells adhering to both polymers, C. freundii adhering on polyethylene displayed a >2-fold, and on polypropylene a >6-fold upregulation of slt-II. Thus, the two new isolates are potential pathogens owing to their drug resistance, surface colonization and upregulation of a slt-II-type diarrheal toxin on polymer surfaces.

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Section: Discussionmentioning

confidence: 99%

Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria, Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669, Isolated from Endangered Turtles (Clemmys guttata)

et al. 2020

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“…This technique revealed the V. cholerae strain(s) responsible for the outbreak may have been accidentally introduced from another geographical location (Chin et al, 2011;Hendriksen et al, 2011). Recently, molecular techniques including WGS have also been applied in other studies involving fermenters in traditional foods and pathogens in vegetable samples of LMIC origin (Adewumi, Oguntoyinbo, Keisam, Romi, & Jeyaram, 2013;Diaz et al, 2018;Ezekiel, Ayeni, et al, 2019;Igbinosa et al, 2018). These studies have mostly been conducted in collaboration with institutions in the North (Western world) where facilities and expertise are readily available; thus, technology and expertise transfer to LMICs are expected in the future.…”

Section: Detection Of Contaminants Of Microbiological Origin In Rtesmentioning

confidence: 99%

Microbiological safety of ready‐to‐eat foods in low‐ and middle‐income countries: A comprehensive 10‐year (2009 to 2018) review

Makinde

Ayeni

Sulyok

et al. 2020

Comp Rev Food Sci Food Safe

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Ready‐to‐eat foods (RTEs) are foods consumed without any further processing. They are widely consumed as choice meals especially by school‐aged children and the fast‐paced working class in most low‐ and middle‐income countries (LMICs), where they contribute substantially to the dietary intake. Depending on the type of processing and packaging material, RTEs could be industrially or traditionally processed. Typically, RTE vendors are of low literacy level, as such, they lack knowledge about good hygiene and food handling practices. In addition, RTEs are often vended in outdoor environments such that they are exposed to several contaminants of microbial origin. Depending on the quantity and type of food contaminant, consumption of contaminated RTEs may result in foodborne diseases and several other adverse health effects in humans. This could constitute major hurdles to growth and development in LMICs. Therefore, this review focuses on providing comprehensive and recent occurrence and impact data on the frequently encountered contaminants of microbial origin published in LMICs within the last decade (2009 to 2018). We have also suggested viable food safety solutions for preventing and controlling the food contamination and promoting consumer health.

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“…Citrobacter portucalensis was first reported from aquatic ecosystems [ 23 ], but multidrug resistant strains have been reported from poultry [ 24 ] and green leafy vegetables [ 25 ]. The closely related Citrobacter freundii is increasingly a nosocomial and environmental pathogen which causes pneumonia, diarrhea, urinary tract, bloodstream, and invasive brain infections [ 5 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669—Potential Zoonotic Pathogens Isolated from Spotted Turtles

et al. 2020

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Antimicrobial resistance (AMR) is one of the biggest challenges of the 21st century, and biofilm formation enables bacteria to resist antibiotic at much higher concentrations than planktonic cells. Earlier, we showed that the Gram-negative Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669 (closely related to C. freundii NBRC 12681) from infected spotted turtles (Clemmys guttata), formed biofilms and upregulated toxin expression on plastic surfaces, and were predicted to possess multiple antibiotic resistance genes. Here, we show that they each resist several antibiotics in the planktonic phase, but were susceptible to neomycin, and high concentrations of tetracycline and cotrimoxazole. The susceptibility of their biofilms to neomycin and cotrimoxazole was tested using the Calgary device. For A. hydrophila, the minimum inhibitory concentration (MIC) = 500–1000, and the minimum biofilm eradication concentration (MBEC) > 1000 μg/mL, using cotrimoxazole, and MIC = 32.3–62.5, and MBEC > 1000 μg/mL, using neomycin. For C. freundii MIC = 7.8–15.6, and, MBEC > 1000 μg/mL, using cotrimoxazole, and MIC = 7.8, and MBEC > 1000 μg/mL, using neomycin. Both A. hydrophila and C. portucalensis activated an acyl homoserine lactone (AHL) dependent biosensor, suggesting that quorum sensing could mediate biofilm formation. Their multidrug resistance in the planktonic form, and weak biofilm eradication even with neomycin and cotrimoxazole, indicate that A. hydrophila and C. portucalensis are potential zoonotic pathogens, with risks for patients living with implants.

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Draft Genome Sequence of Multidrug-Resistant Strain Citrobacter portucalensis MBTC-1222, Isolated from Uziza (Piper guineense) Leaves in Nigeria

Cited by 12 publications

References 9 publications

Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria, Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669, Isolated from Endangered Turtles (Clemmys guttata)

Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria, Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669, Isolated from Endangered Turtles (Clemmys guttata)

Microbiological safety of ready‐to‐eat foods in low‐ and middle‐income countries: A comprehensive 10‐year (2009 to 2018) review

Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669—Potential Zoonotic Pathogens Isolated from Spotted Turtles

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