Draft Genome Sequence of Moraxella bovoculi Strain KZ-1, Isolated from Cattle in North Kazakhstan

Куйбагаров, Марат; Amirgazin, Asylulan; Vergnaud, Gilles; Shustov, Alexandr V.; Ryskeldina, Anara; Ramankulov, Yerlan; Shevtsov, Alexandr

doi:10.1128/mra.00670-20

Cited by 3 publications

(5 citation statements)

References 9 publications

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“…A possible reason for the high prevalence of group A in this study is the limited region and sampling period, as well as the relatively recent registration of IBK in the surveyed farms (reported since 2016). However, this was also the most common PilA group previously reported by Kuibagarov et al [3] and Angelos et al [23] among Mor. bovoculi in the United States and Kazakhstan, although in those studies, the proportion did not exceed 68%.…”

Section: Discussionsupporting

confidence: 62%

“…However, this was also the most common PilA group previously reported by Kuibagarov et al . [ 3 ] and Angelos et al . [ 23 ] among Mor.…”

Section: Discussionmentioning

confidence: 99%

“…PilA-deduced amino acid sequences were also compared to previously published by Kuibagarov et al . [ 3 ] and Angelos et al . [ 23 ] PilA sequences for Mor.…”

Section: Methodsmentioning

confidence: 99%

“…The deduced amino acid sequences obtained from pilA amplicons were compared to sequences in GenBank using the Basic Local Alignment Search Tool algorithm, and phylogenetic trees were built based on comparisons with these sequences. PilA-deduced amino acid sequences were also compared to previously published by Kuibagarov et al [3] and Angelos et al [23] PilA sequences for Mor. bovoculi.…”

Section: Polymerase Chain Reaction Testing; Sequencing Of Mor Bovocul...mentioning

confidence: 99%

“…Infectious bovine keratoconjunctivitis (IBK) is a contagious ocular disease of cattle characterized by ulcerative keratitis, corneal edema, and conjunctivitis. This disease is the most common eye condition of cattle [1] and occurs worldwide, including in Kazakhstan [2][3][4]. Although corneal ulcerations in cattle may heal with limited corneal scar formation, it is not uncommon for IBK to result in complete or partial loss of vision [5].…”

Section: Introductionmentioning

confidence: 99%

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Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan

Kuibagarov,

Abdullina,

Ryskeldina

et al. 2023

Vet World

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Background and Aim: Infectious bovine keratoconjunctivitis (IBK) causes a significant economic loss to cattle industries in many countries, including Kazakhstan. Although Moraxella bovis is recognized as an etiologic agent of IBK, other bacterial and viral agents have been suspected to play a role in the pathogenesis of this disease. This study aimed to evaluate samples collected from the eyes of IBK-affected cattle in Eastern Kazakhstan at different stages of IBK for the presence of Mor. bovis, Moraxella bovoculi, Mycoplasma bovis, Mycoplasma bovoculi, and Bovine Herpes Virus Type 1 (BHV-1) and to characterize Mor. bovoculi pilA gene sequence diversity from Mor. bovoculi positive samples. Materials and Methods: Individual ocular swabs (n = 168) were collected from cattle that had clinical signs of IBK during the summer of 2022 on farms in the Abay region of Kazakhstan. Eye lesion scores (1, 2, and 3) were assigned depending on the degree of ocular damage. Infectious bovine keratoconjunctivitis-associated organisms were detected using a multiplex real-time polymerase chain reaction assay. The Mor. bovoculi pilA gene was sequenced from Mor. bovoculi positive samples. Results: Mycoplasma bovis and BHV-1 were not detected in any of the collected samples. Mycoplasma bovoculi was identified in the majority of samples overall, usually in mixed infection with Moraxella spp. Moraxella bovoculi was detected in 76.2% of animals and predominated in animals with eye lesion scores 2 and 3. Mycoplasma bovoculi was detected only in association with Mor. bovis and/or Mor. bovoculi in animals with eye lesion scores 2 and 3. Moraxella bovis was found in 57.7% of animals and was always identified in association with another organism. Sequencing of the pilA gene in 96 samples from Mor. bovoculi positive samples identified five PilA groups. The majority belonged to PilA group A. However, three new PilA groups were identified and designated PilA groups N, O, and P. Conclusion: The results indicate a high prevalence of Myc. bovoculi and Mor. bovoculi in eyes of cattle with IBK on livestock farms in Eastern Kazakhstan. Additional novel Mor. bovoculi PilA groups were identified. Keywords: bovine herpes virus, infectious bovine keratoconjunctivitis, Moraxella bovis, Moraxella bovoculi, multiplex real-time polymerase chain reaction, Mycoplasma bovis, Mycoplasma bovoculi.

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Section: Discussionsupporting

confidence: 62%

“…However, this was also the most common PilA group previously reported by Kuibagarov et al . [ 3 ] and Angelos et al . [ 23 ] among Mor.…”

Section: Discussionmentioning

confidence: 99%

“…PilA-deduced amino acid sequences were also compared to previously published by Kuibagarov et al . [ 3 ] and Angelos et al . [ 23 ] PilA sequences for Mor.…”

Section: Methodsmentioning

confidence: 99%

Section: Polymerase Chain Reaction Testing; Sequencing Of Mor Bovocul...mentioning

confidence: 99%