Draft Genome Sequence of Microbacterium oleivorans Strain A9, a Bacterium Isolated from Chernobyl Radionuclide-Contaminated Soil

Abstract

Here, we present the draft genome sequence of Microbacterium oleivorans strain A9, a uranium-tolerant actinobacterium which has been isolated from radionuclide-contaminated soil from the Chernobyl exclusion zone. It is composed of 22 contigs totaling 2,954,335 bp and contains 2,813 coding DNA sequences, one cluster of rRNA genes, and 45 tRNA genes.

Proteogenomic insights into uranium tolerance of a Chernobyl's Microbacterium bacterial isolate

“…(including 15% of membrane proteins) of the predicted protein-coding genes found in the draft genome of Microbacterium oleivorans A9 [38]. The distribution of the peptides detected during our experiment along the chimeric genome of Microbacterium oleivorans A9 is uniform and confirm the validity of this proteogenomics approach, as previously discussed for the proteogenomics mapping of the alphaproteobacterium Tistlia consotensis [22].…”

Complete Genome Sequences of Four Microbacterium Strains Isolated from Metal- and Radionuclide-Rich Soils

“…The Microbacterium oleivorans strain A9 was isolated from radionuclide-contaminated soil in Chernobyl (8) and exhibits a high uranium tolerance (9). Its draft genome sequence was previously published (10). The strains ViU2A and ViU22 T were isolated from natural uranium-rich soil samples collected in France (the soil composition is described in reference 11; the strain ViU22 T is the type strain of the species Microbacterium lemovicicum [12]).…”

Discovery and characterization of UipA, a uranium- and iron-binding PepSY protein involved in uranium tolerance by soil bacteria

“…Using tightly controlled exposure conditions, a 3-step process involving uranyl sorption, release and biomineralization was evidenced (44). Using proteogenomics, we further showed the high impact of uranyl on the proteome of this strain (45,46). In the present work, we have examined three additional Microbacterium strains for which we obtained the whole-genome (47), and subjected them to comparative analysis (Fig.…”