Draft Genome Sequence of Marine Sponge Symbiont Pseudoalteromonas luteoviolacea IPB1, Isolated from Hilo, Hawaii

Sakai-Kawada, Francis E.; Yakym, Christopher J.; Helmkampf, Martin; Hagiwara, Kehau A.; Ip, Courtney G.; Antonio, Brandi; Armstrong, Ellie E.; Ulloa, Wesley J.; Awaya, Jonathan D.

doi:10.1128/genomea.01002-16

Cited by 4 publications

(5 citation statements)

References 14 publications

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“…From the three species described, P. denitrificans and P. rubra were isolated from seawater samples, while P. tunicata was characterized in marine algae and tunicates (Bowman, 2007). There is a close association between pigmented Pseudoalteromonas species and marine macroorganisms (Zheng et al, 2006; Sakai-Kawada et al, 2016). One study isolated P. tunicata from a healthy adult tunicate and Ulva lactuca , a common green alga; both hosts did not independently produce antifouling compounds.…”

Section: Evolutionary and Ecological Significancementioning

confidence: 99%

“…Pseudoalteromonas has also been reported to produce tambjamines, a yellow pigment that is structurally like prodiginine, sharing two of the three pyrrole rings (Burke et al, 2007). The presence of pigmented metabolites like prodiginine and tambjamines in species of Pseudoalteromonas is particularly interesting because the genus is commonly associated with macroorganisms, such as algae (Dobretsov and Qian, 2002), fish (Pratheepa and Vasconcelos, 2013), sponges (Sakai-Kawada et al, 2016), and tunicates (Holmström et al, 1998). This host-microbe interaction provides the microorganism with nutrients to flourish, while providing the host with valuable resources that may contribute to nutrient cycling or defense (Webster and Taylor, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis and Bioactivity of Prodiginine Analogs in Marine Bacteria, Pseudoalteromonas: A Mini Review

Sakai-Kawada

Hagiwara

et al. 2019

Front. Microbiol.

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The Prodiginine family consists of primarily red-pigmented tripyrrole secondary metabolites that were first characterized in the Gram-negative bacterial species Serratia marcescens and demonstrates a wide array of biological activities and applications. Derivatives of prodiginine have since been characterized in the marine γ-proteobacterium, Pseudoalteromonas . Although biosynthetic gene clusters involved in prodiginine synthesis display homology among genera, there is an evident structural difference in the resulting metabolites. This review will summarize prodiginine biosynthesis, bioactivity, and gene regulation in Pseudoalteromonas in comparison to the previously characterized species of Serratia , discuss the ecological contributions of Pseudoalteromonas in the marine microbiome and their eukaryotic hosts, and consider the importance of modern functional genomics and classic DNA manipulation to understand the overall prodiginine biosynthesis pathway.

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Section: Evolutionary and Ecological Significancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Bioactivity of Prodiginine Analogs in Marine Bacteria, Pseudoalteromonas: A Mini Review

Sakai-Kawada

Hagiwara

et al. 2019

Front. Microbiol.

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“…In our global analyses, the higher abundance of Pseudoalteromonas appeared to be unique to the larvae of P. cyanorosea when compared to other Homoscleromorpha specimens. Nevertheless, cultivable strains with specialized genomic content encoding for properties that may be involved in a symbiotic lifestyle have previously been reported from both the Vibrio [ 75 ] and Pseudoalteromonas [ 76 , 77 ] strains isolated from sponges. Therefore, a possible long-term association with the sponge host and the likelihood of maternal transmission also occurring be running in P. cyanorosea cannot be completely discarded for these gammaproteobacterial genera.…”

Section: Discussionmentioning

confidence: 99%

Not That Close to Mommy: Horizontal Transmission Seeds the Microbiome Associated with the Marine Sponge Plakina cyanorosea

et al. 2020

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Marine sponges are excellent examples of invertebrate–microbe symbioses. In this holobiont, the partnership has elegantly evolved by either transmitting key microbial associates through the host germline and/or capturing microorganisms from the surrounding seawater. We report here on the prokaryotic microbiota during different developmental stages of Plakina cyanorosea and their surrounding environmental samples by a 16S rRNA metabarcoding approach. In comparison with their source adults, larvae housed slightly richer and more diverse microbial communities, which are structurally more related to the environmental microbiota. In addition to the thaumarchaeal Nitrosopumilus, parental sponges were broadly dominated by Alpha- and Gamma-proteobacteria, while the offspring were particularly enriched in the Vibrionales, Alteromonodales, Enterobacterales orders and the Clostridia and Bacteroidia classes. An enterobacterial operational taxonomic unit (OTU) was the dominant member of the strict core microbiota. The most abundant and unique OTUs were not significantly enriched amongst the microbiomes from host specimens included in the sponge microbiome project. In a wider context, Oscarella and Plakina are the sponge genera with higher divergence in their associated microbiota compared to their Homoscleromorpha counterparts. Our results indicate that P. cyanorosea is a low microbial abundance sponge (LMA), which appears to heavily depend on the horizontal transmission of its microbial partners that likely help the sponge host in the adaptation to its habitat.

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“…Template enrichment was performed on the Ion OneTouch Enrichment System. The sample was loaded into an Ion 318 chip v2 and sequenced using the Ion PGM Hi-Q sequencing kit on the Ion PGM system (Sakai-Kawada et al, 2016).…”

Section: Ferric Reducing Antioxidant Power (Frap) Assaymentioning

confidence: 99%

“…The bacterial genus, Pseudoalteromonas has been associated with harboring within various hosts as well as with producing numerous bioactive pigments (Bowman, 2007;Ramesh et al, 2019b;Sakai-Kawada et al, 2019). One example includes Pseudoalteromonas luteoviolacea which was isolated from a marine sponge, Iotrochota protea, and was shown to produce the pigment, violacein (Sakai-Kawada et al, 2016). Violacein has demonstrated both antibiotic and antiprotozoal activities that could benefit both itself and its host (Soliev et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Prodiginine Pathway in Marine Sponge-Associated Pseudoalteromonas sp. PPB1 in Hilo, Hawai‘i

Sakai-Kawada

Hagiwara

et al. 2020

Front. Sustain. Food Syst.

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Interest in bioactive pigments stems from their ecological role in adaptation, as well as their applications in various consumer products. The production of these bioactive pigments can be from a variety of biological sources, including simple microorganisms that may or may not be associated with a host. This study is particularly interested in the marine sponges, which have been known to harbor microorganisms that produce secondary metabolites like bioactive pigments. In this study, marine sponge tissue samples were collected from Puhi Bay off the Eastern shore of Hilo, Hawai‘i and subsequently were identified as Petrosia sp. with red pigmentation. Using surface sterilization and aseptic plating of sponge tissue samples, sponge-associated microorganisms were isolated. One isolate (PPB1) produced a colony with red pigmentation like that of Petrosia sp., suggesting an integral relationship between this particular isolate and the sponge of interest. 16S characterization and sequencing of PPB1 revealed that it belonged to the Pseudoalteromonas genus. Using various biological assays, both antimicrobial and antioxidant bioactivity was shown in Pseudoalteromonas sp. PPB1 crude extract. To further investigate the genetics of pigment production, a draft genome of PPB1 was sequenced, assembled, and annotated. This revealed a prodiginine biosynthetic pathway and the first cited-incidence of a prodiginine-producing Pseudoalteromonas species isolated from a marine sponge host. Further understanding into the bioactivity and biosynthesis of secondary metabolites like pigmented prodiginine may uncover the complex ecological interactions between host sponge and microorganism.

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Draft Genome Sequence of Marine Sponge Symbiont Pseudoalteromonas luteoviolacea IPB1, Isolated from Hilo, Hawaii

Cited by 4 publications

References 14 publications

Biosynthesis and Bioactivity of Prodiginine Analogs in Marine Bacteria, Pseudoalteromonas: A Mini Review

Biosynthesis and Bioactivity of Prodiginine Analogs in Marine Bacteria, Pseudoalteromonas: A Mini Review

Not That Close to Mommy: Horizontal Transmission Seeds the Microbiome Associated with the Marine Sponge Plakina cyanorosea

Characterization of Prodiginine Pathway in Marine Sponge-Associated Pseudoalteromonas sp. PPB1 in Hilo, Hawai‘i

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