Draft Genome Sequence of &lt;I&gt;Cercospora canescens&lt;/I&gt;: A Leaf Spot Causing Pathogen

Chand, Ramesh; Pal, Chhattar; Singh, Vineeta; Kumar, M.; Singh, Vinay Kumar; Chowdappa, P.

doi:10.18520/v109/i11/2103-2110

Cited by 13 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These reads were assembled to contigs which were connected to 2618 scaffolds (>500 bp) with an N50 size of 243.6 kb and a total assembly length of 66.6 Mb ( Table 2 ). These data are comparable to other fungal draft genomes [ 8 , 9 , 26 ] but especially the N50 value and the size of the longest scaffold (approximately 1 Mb) are outstandingly long and much larger than for others assemblies generated using similar sequence datasets. Nevertheless the genome is still very fragmented even if one takes only larger scaffolds with more than 500 bp into account.…”

Section: Resultssupporting

confidence: 81%

“…The PhytoPath [ 5 ] database contains more than 80 genomes of phytopathogenic fungi, which have been sequenced to completion, allowing crucial insights into genomic adaptations to parasitic or hemiparasitic lifestyles [ 6 , 7 ]. In addition to the assembly of full genomes, draft genomes with more fragmented assemblies are very useful tools in capturing the gene space of a particular species and to identify factors relevant for host pathogen interactions [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A draft genome sequence of the rose black spot fungus Diplocarpon rosae reveals a high degree of genome duplication

et al. 2017

View full text Add to dashboard Cite

BackgroundBlack spot is one of the most severe and damaging diseases of garden roses. We present the draft genome sequence of its causative agent Diplocarpon rosae as a working tool to generate molecular markers and to analyze functional and structural characteristics of this fungus.ResultsThe isolate DortE4 was sequenced with 191x coverage of different read types which were assembled into 2457 scaffolds. By evidence supported genome annotation with the MAKER pipeline 14,004 gene models were predicted and transcriptomic data indicated that 88.5% of them are expressed during the early stages of infection. Analyses of k-mer distributions resulted in unexpectedly large genome size estimations between 72.5 and 91.4 Mb, which cannot be attributed to its repeat structure and content of transposable elements alone, factors explaining such differences in other fungal genomes. In contrast, different lines of evidences demonstrate that a huge proportion (approximately 80%) of genes are duplicated, which might indicate a whole genome duplication event. By PCR-RFLP analysis of six paralogous gene pairs of BUSCO orthologs, which are expected to be single copy genes, we could show experimentally that the duplication is not due to technical error and that not all isolates tested possess all of the paralogs.ConclusionsThe presented genome sequence is still a fragmented draft but contains almost the complete gene space. Therefore, it provides a useful working tool to study the interaction of D. rosae with the host and the influence of a genome duplication outside of the model yeast in the background of a phytopathogen.

show abstract

Section: Resultssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

A draft genome sequence of the rose black spot fungus Diplocarpon rosae reveals a high degree of genome duplication

et al. 2017

View full text Add to dashboard Cite

show abstract

“…are not well represented in public databases. Therefore, to aid comparative analysis within the Cercospora genus, we also sequenced the genome of Cercospora berteroae and reassembled the genome of Cercospora canescens ( 24 ) ( SI Appendix , Table S1 ). To identify gene clusters responsible for biosynthesis of aromatic polyketides in C. beticola , we mined the genome to identify all SM clusters ( 25 ) and compared these with predicted clusters in related Dothideomycetes.…”

Section: Resultsmentioning

confidence: 99%

Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum

Jonge

Ebert

Huitt-Roehl

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

124

View full text Add to dashboard Cite

SignificanceSpecies in the fungal genus Cercospora cause diseases in many important crops worldwide. Their success as pathogens is largely due to the secretion of cercosporin during infection. We report that the cercosporin toxin biosynthesis (CTB) gene cluster is ancient and was horizontally transferred to diverse fungal plant pathogens. Because our analyses revealed genes adjacent to the established CTB cluster with similar evolutionary trajectories, we evaluated their role in Cercospora beticola to show that four are necessary for cercosporin biosynthesis. Lastly, we confirmed that the apple pathogen Colletotrichum fioriniae produces cercosporin, the first case outside the family Mycosphaerellaceae. Other Colletotrichum plant pathogens also harbor the CTB cluster, which points to a wider role that this toxin may play in virulence.

show abstract

“…The estimated genome size of C. beticola (37 Mb) is comparable to that of multiple Cercospora species, including C. canescens (~34 Mb; Chand et al . 2015 ), C. cf.…”

Section: Resultsmentioning

confidence: 99%

Draft genome sequence of Annulohypoxylon stygium, Aspergillus mulundensis, Berkeleyomyces basicola (syn. Thielaviopsis basicola), Ceratocystis smalleyi, two Cercospora beticola strains, Coleophoma cylindrospora, Fusarium fracticaudum, Phialophora cf. hyalina, and Morchella septimelata

et al. 2018

View full text Add to dashboard Cite

Draft genomes of the species Annulohypoxylon stygium, Aspergillus mulundensis, Berkeleyomyces basicola (syn. Thielaviopsis basicola), Ceratocystis smalleyi, two Cercospora beticola strains, Coleophoma cylindrospora, Fusarium fracticaudum, Phialophora cf. hyalina and Morchella septimelata are presented. Both mating types (MAT1-1 and MAT1-2) of Cercospora beticola are included. Two strains of Coleophoma cylindrospora that produce sulfated homotyrosine echinocandin variants, FR209602, FR220897 and FR220899 are presented. The sequencing of Aspergillus mulundensis, Coleophoma cylindrospora and Phialophora cf. hyalina has enabled mapping of the gene clusters encoding the chemical diversity from the echinocandin pathways, providing data that reveals the complexity of secondary metabolism in these different species. Overall these genomes provide a valuable resource for understanding the molecular processes underlying pathogenicity (in some cases), biology and toxin production of these economically important fungi.

show abstract

Draft Genome Sequence of <I>Cercospora canescens</I>: A Leaf Spot Causing Pathogen

Cited by 13 publications

References 0 publications

A draft genome sequence of the rose black spot fungus Diplocarpon rosae reveals a high degree of genome duplication

A draft genome sequence of the rose black spot fungus Diplocarpon rosae reveals a high degree of genome duplication

Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum

Draft genome sequence of Annulohypoxylon stygium, Aspergillus mulundensis, Berkeleyomyces basicola (syn. Thielaviopsis basicola), Ceratocystis smalleyi, two Cercospora beticola strains, Coleophoma cylindrospora, Fusarium fracticaudum, Phialophora cf. hyalina, and Morchella septimelata

Contact Info

Product

Resources

About