Draft genome sequence of Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230: a tool for preliminary identification of enzymes involved in CLA metabolism

Oleate Hydratase in Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230 Catalyzes the Reversible Conversion between Linoleic Acid and Ricinoleic Acid

“…However, the genome sequencing of L. delbrueckii subsp. bulgaricus LBP UFSC 2230 suggested that only one gene capable of encoding an oleate hydratase ( oleH ) enzyme ( 14 ) based on homology testing. In a previous study, our group identified that the production of the CLA isomers in a coculture of L. delbrueckii subsp.…”

“…Since annotation of any putative linoleate isomerase in the L. delbrueckii subsp. bulgaricus LBP UFSC 2230 genome ( 14 ) was not evident, more studies will be carried out in the future to fully unravel the complex mechanisms and pathways involved in CLA production in this strain.…”

“…In our previous work, genome sequencing of Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230 (BioProject accession number PRJNA615231 ; SRA accession number SRR11741240 ) confirmed the presence of a single gene ( oleH ) capable of expressing an enzyme which was identified as an oleate hydratase and not the presence of other genes which are typically found in other such lactobacilli strains, generally involved in this multienzyme process ( 14 ).…”