Draft Genome Sequence of Labrenzia sp. Strain EL143, a Coral-Associated Alphaproteobacterium with Versatile Symbiotic Living Capability and Strong Halogen Degradation Potential

Rodrigues, Gisele Nunes; Lago‐Lestón, Asunción; Costa, Rodrigo; Keller‐Costa, Tina

doi:10.1128/genomea.00132-18

Cited by 9 publications

(11 citation statements)

References 12 publications

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“…strain EL138 [117], and Labrenzia sp. strain EL143 [118], suggested versatile secondary metabolisms. Using antiSMASH v.3 [119], we have now mined 11 genomes representing different bacterial genera isolated from E. labiata by Keller-Costa et al [44], in addition to four genomes from Vibrio strains associated with healthy and diseased Eunicella verrucosa specimens [120,121], for the presence of secondary metabolite biosynthetic genes clusters (BGCs).…”

Section: Genomic Insights Into Natural Product Biosynthesis By Bacmentioning

confidence: 99%

Bioactive Secondary Metabolites from Octocoral-Associated Microbes—New Chances for Blue Growth

et al. 2018

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Octocorals (Cnidaria, Anthozoa Octocorallia) are magnificent repositories of natural products with fascinating and unusual chemical structures and bioactivities of interest to medicine and biotechnology. However, mechanistic understanding of the contribution of microbial symbionts to the chemical diversity of octocorals is yet to be achieved. This review inventories the natural products so-far described for octocoral-derived bacteria and fungi, uncovering a true chemical arsenal of terpenes, steroids, alkaloids, and polyketides with antibacterial, antifungal, antiviral, antifouling, anticancer, anti-inflammatory, and antimalarial activities of enormous potential for blue growth. Genome mining of 15 bacterial associates (spanning 12 genera) cultivated from Eunicella spp. resulted in the identification of 440 putative and classifiable secondary metabolite biosynthetic gene clusters (BGCs), encompassing varied terpene-, polyketide-, bacteriocin-, and nonribosomal peptide-synthase BGCs. This points towards a widespread yet uncharted capacity of octocoral-associated bacteria to synthetize a broad range of natural products. However, to extend our knowledge and foster the near-future laboratory production of bioactive compounds from (cultivatable and currently uncultivatable) octocoral symbionts, optimal blending between targeted metagenomics, DNA recombinant technologies, improved symbiont cultivation, functional genomics, and analytical chemistry are required. Such a multidisciplinary undertaking is key to achieving a sustainable response to the urgent industrial demand for novel drugs and enzyme varieties.

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Section: Genomic Insights Into Natural Product Biosynthesis By Bacmentioning

confidence: 99%

Bioactive Secondary Metabolites from Octocoral-Associated Microbes—New Chances for Blue Growth

et al. 2018

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“…The Rhodobacteraceae family has been isolated mainly from marine habitats, in association with marine invertebrates such as corals, colonizing the surfaces of oysters and shells and in the rhizosphere of halophytes plants from coastal areas [8][9][10]. Labrenzia spp., a member of this family, possess biosynthetic genes for the production of non-ribosomal peptides (NRPS), polyketide synthases (PKS), bacteriocins and terpenoids [9,11], but few secondary metabolites have been isolated. To date, the described compounds are a polyketide pederin analogue, isolated from a free-living marine Labrenzia sp.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Potential of Extracts of Labrenzia aggregata Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source

et al. 2020

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Previous studies revealed the potential of Labrenzia aggregata USBA 371 to produce cytotoxic metabolites. This study explores its metabolic diversity and compounds involved in its cytotoxic activity. Extracts from the extracellular fraction of strain USBA 371 showed high levels of cytotoxic activity associated with the production of diketopiperazines (DKPs). We purified two compounds and a mixture of two other compounds from this fraction. Their structures were characterized by 1D and 2D nuclear magnetic resonance (NMR). The purified compounds were evaluated for additional cytotoxic activities. Compound 1 (cyclo (l-Pro-l-Tyr)) showed cytotoxicity to the following cancer cell lines: breast cancer 4T1 (IC50 57.09 ± 2.11 µM), 4T1H17 (IC50 40.38 ± 1.94), MCF-7 (IC50 87.74 ± 2.32 µM), murine melanoma B16 (IC50 80.87 ± 3.67), human uterus sarcoma MES-SA/Dx5 P-pg (−) (IC50 291.32 ± 5.64) and MES-SA/Dx5 P-pg (+) (IC50 225.28 ± 1.23), and murine colon MCA 38 (IC50 29.85 ± 1.55). In order to elucidate the biosynthetic route of the production of DKPs and other secondary metabolites, we sequenced the genome of L. aggregata USBA 371. We found no evidence for biosynthetic pathways associated with cyclodipeptide synthases (CDPSs) or non-ribosomal peptides (NRPS), but based on proteogenomic analysis we suggest that they are produced by proteolytic enzymes. This is the first report in which the cytotoxic effect of cyclo (l-Pro-l-Tyr) produced by an organism of the genus Labrenzia has been evaluated against several cancer cell lines.

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“…Labrenzia species are Gram-negative, aerobic, motile, and rod-shaped bacteria of the class Alphaproteobacteria. They require NaCl for growth and are isolated from marine habitats, in many reported cases, in association with marine invertebrates such as corals [5][6][7]. Although the species of Labrenzia possess biosynthetic genes for the production of polyketide, non-ribosomal peptide, and terpenoid [7], very little is known about their secondary metabolites.…”

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confidence: 99%

“…They require NaCl for growth and are isolated from marine habitats, in many reported cases, in association with marine invertebrates such as corals [5][6][7]. Although the species of Labrenzia possess biosynthetic genes for the production of polyketide, non-ribosomal peptide, and terpenoid [7], very little is known about their secondary metabolites. To date, a polyketide-derived pederin analog and two cyclopropanecontaining fatty acids have been reported [8,9].…”

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Labrenzbactin from a coral-associated bacterium Labrenzia sp.

et al. 2019

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A new catecholate-containing siderophore, labrenzbactin (1), was isolated from the fermentation broth of a coral-associated bacterium Labrenzia sp. The structure and absolute configuration of 1 was determined by spectroscopic methods and Marfey's analysis. Overall, 1 showed antimicrobial activity against Ralstonia solanacearum SUPP1541 and Micrococcus luteus ATCC9341 with MIC values of 25 and 50 µg ml −1 , respectively, and cytotoxicity against P388 murine leukemia cells with an IC 50 of 13 µM. 1234567890();,:1234567890();,:

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Draft Genome Sequence of Labrenzia sp. Strain EL143, a Coral-Associated Alphaproteobacterium with Versatile Symbiotic Living Capability and Strong Halogen Degradation Potential

Cited by 9 publications

References 12 publications

Bioactive Secondary Metabolites from Octocoral-Associated Microbes—New Chances for Blue Growth

Bioactive Secondary Metabolites from Octocoral-Associated Microbes—New Chances for Blue Growth

Bioactive Potential of Extracts of Labrenzia aggregata Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source

Labrenzbactin from a coral-associated bacterium Labrenzia sp.

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