Draft Genome Sequence of
            <i>Saccharomycopsis fodiens</i>
            CBS 8332, a Necrotrophic Mycoparasite with Biocontrol Potential

Junker, Klara; Hesselbart, Ana; Wendland, Jürgen

doi:10.1128/genomea.01278-17

Cited by 9 publications

(5 citation statements)

References 15 publications

(10 reference statements)

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“…Similarly, the yeast-like Ustilaginomycete Pseudozyma aphidis parasitises the powdery mildew pathogen Podosphaera xanthii and B. cinerea (Calderon et al 2019; Gafni et al 2015). The genus Saccharomycopsis , comprising predacious yeasts directly feeding on their prey, was studied with respect to biocontrol of different Penicillium species as well as clinically relevant yeasts (Junker et al 2017, 2018, 2019; Lachance and Pang 1997; Pimenta et al 2008).…”

Section: Mechanisms Underlying the Biocontrol Activity Of Yeastsmentioning

confidence: 99%

Biocontrol yeasts: mechanisms and applications

Freimoser

Rueda‐Mejia

Tilocca

et al. 2019

World J Microbiol Biotechnol

290

190

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Yeasts occur in all environments and have been described as potent antagonists of various plant pathogens. Due to their antagonistic ability, undemanding cultivation requirements, and limited biosafety concerns, many of these unicellular fungi have been considered for biocontrol applications. Here, we review the fundamental research on the mechanisms (e.g., competition, enzyme secretion, toxin production, volatiles, mycoparasitism, induction of resistance) by which biocontrol yeasts exert their activity as plant protection agents. In a second part, we focus on five yeast species (Candida oleophila, Aureobasidium pullulans, Metschnikowia fructicola, Cryptococcus albidus, Saccharomyces cerevisiae) that are or have been registered for the application as biocontrol products. These examples demonstrate the potential of yeasts for commercial biocontrol usage, but this review also highlights the scarcity of fundamental studies on yeast biocontrol mechanisms and of registered yeast-based biocontrol products. Yeast biocontrol mechanisms thus represent a largely unexplored field of research and plentiful opportunities for the development of commercial, yeast-based applications for plant protection exist.

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Section: Mechanisms Underlying the Biocontrol Activity Of Yeastsmentioning

confidence: 99%

Biocontrol yeasts: mechanisms and applications

Freimoser

Rueda‐Mejia

Tilocca

et al. 2019

World J Microbiol Biotechnol

290

190

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“…Just like we were unable to detect any genes in the sulfate assimilation pathway in the genomes of S . fodiens [27] and S . fermentans [28], the genes for sulfate uptake and reduction were all absent in the draft genome of S .…”

Section: Resultsmentioning

confidence: 99%

“…fermentans , but only one tRNA-CAG gene in the S . fodiens genome [27, 28]. We aligned these Saccharomycopsis tRNA(CAG) genes with serine and leucine tRNA genes from S .…”

Section: Resultsmentioning

confidence: 99%

“…The vast majority of microorganisms, including yeast, are able to take up sulfur in the form of inorganic sulfate and reduce it in the sulfate assimilation pathway [24], but yeasts in the Saccharomycopsis clade share a rare inability to assimilate inorganic sulfur [25, 26]. We recently reported that in Saccharomycopsis fodiens [27] and Saccharomycopsis fermentans [28], the genomic basis for this rare organic sulfur auxotrophy is a complete absence of all genes in the sulfate assimilation pathway.…”

Section: Introductionmentioning

confidence: 99%

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Multi-omics characterization of the necrotrophic mycoparasite Saccharomycopsis schoenii

et al. 2019

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Pathogenic yeasts and fungi are an increasing global healthcare burden, but discovery of novel antifungal agents is slow. The mycoparasitic yeast Saccharomycopsis schoenii was recently demonstrated to be able to kill the emerging multi-drug resistant yeast pathogen Candida auris . However, the molecular mechanisms involved in the predatory activity of S . schoenii have not been explored. To this end, we de novo sequenced, assembled and annotated a draft genome of S . schoenii . Using proteomics, we confirmed that Saccharomycopsis yeasts have reassigned the CTG codon and translate CTG into serine instead of leucine. Further, we confirmed an absence of all genes from the sulfate assimilation pathway in the genome of S . schoenii , and detected the expansion of several gene families, including aspartic proteases. Using Saccharomyces cerevisiae as a model prey cell, we honed in on the timing and nutritional conditions under which S . schoenii kills prey cells. We found that a general nutrition limitation, not a specific methionine deficiency, triggered predatory activity. Nevertheless, by means of genome-wide transcriptome analysis we observed dramatic responses to methionine deprivation, which were alleviated when S . cerevisiae was available as prey, and therefore postulate that S . schoenii acquired methionine from its prey cells. During predation, both proteomic and transcriptomic analyses revealed that S . schoenii highly upregulated and translated aspartic protease genes, probably used to break down prey cell walls. With these fundamental insights into the predatory behavior of S . schoenii , we open up for further exploitation of this yeast as a biocontrol yeast and/or source for novel antifungal agents.

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“…Furthermore, a deeper understanding of the molecular biology of Saccharomycopsis species—specifically of their predacious behavior—is warranted. To this end, we recently established the draft genome of another predacious Saccharomycopsis yeast, S. fodiens strain CBS 8332 ( 9 ). We confirmed that, just like S. fibuligera , S. fodiens also lacks the genes in the sulfate assimilation pathway.…”

Section: Genome Announcementmentioning

confidence: 99%