Draft Genome Sequence of Ralstonia solanacearum Strain Rs-T02, Which Represents the Most Prevalent Phylotype in Guangxi, China

Abstract

Ralstonia solanacearum strain Rs-T02 was originally isolated from a bacterial wilt of tomato plant in Nanning City of Guangxi Province, China. It represents the most prevalent phylotype in Guangxi. Here, we present the draft genome sequence of this strain, which comprises 5,225 genes and 5,976,011 nucleotides with an average G+C content of 66.79%. There are 968 different genes between this isolate and the previously reported genome sequence of Ralstonia solanacearum GMl l000 (race l, biovar 3, phylotype I), and the genome sequence information of this isolate may be useful for comparative genomic studies to determine the genetic diversity in this species.

A yceI Gene Involves in the Adaptation of Ralstonia solanacearum to Methyl Gallate and Other Stresses

“…The wild-type strain, R. solanacearum RS-T02, was originally isolated from a tomato plant in Nanning City, Guangxi Province, China [ 26 ]. The strains were stored in sterilized water at 20 °C.…”

“…The wild-type strain, R. solanacearum RS-T02, was originally isolated from a tomato plant in Nanning City, Guangxi Province, China [26]. The strains were stored in sterilized water at 20 • C. The R. solanacearum strains were initially cu1tured on nutrient agar medium (NA: 3 g/L yeast extract, 5 g/L peptone, 10 g/L glucose, and 18 g/L agar) for 24-48 h or in NA liquid medium (28 • C, 130 r/min) for 24 h. The competent cells of Escherichia coli DH5α for vector construction were grown in Luria-Bertani (LB) medium at 37 • C. The concentration of antibiotics in the used medium was as follows: 25 µg/mL kanamycin (Km) and 50 µg/mL ampicillin (Amp).…”