Draft Genome Sequence of
 Pseudomonas
 sp. Strain LFM046, a Producer of Medium-Chain-Length Polyhydroxyalkanoate

Cardinali-Rezende, Juliana; Alexandrino, Paulo Moises Raduan; Nahat, Rafael A.T.P.S.; Sant’Ana, Débora Parrine Vieira; Silva, Luiziana Ferreira da; Gomez, José Gregório Cabrera; Taciro, Marilda Keico

doi:10.1128/genomea.00966-15

Cited by 9 publications

(3 citation statements)

References 7 publications

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“…This species accumulates at least 60% (w/w) of its biomass as PHA MCL , when grown under nitrogen and phosphate limitation [Gomez, 2000;Sánchez et al, 2003;Diniz et al, 2004;Taciro, 2008;Silva-Queiroz et al, 2009]. Metabolic pathways involved in PHA production by this bacterium have been analyzed based on 13 C-labelling experiments [Riascos et al, 2013], and its genome was recently sequenced and annotated by our research group, confirming that the PHA operon phaC1ZC2 is encoded [Cardinali-Rezende et al, 2015]. Differently from other studies producing P[3HB-co-3HA MCL ], previous studies by our research group have indicated the production of PHA copolymers containing 3HA SCL and 3HA MCL from glucose as the sole carbon source when Ralstonia eutropha or Aeromonas spp.…”

Section: Introductionmentioning

confidence: 83%

Production of Polyhydroxyalkanoates Copolymers by Recombinant Pseudomonas in Plasmid- and Antibiotic-Free Cultures

et al. 2018

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Three different polyhydroxyalkanoate (PHA) synthase genes (Ralstonia eutropha H16, Aeromonas sp. TSM81 or Aeromonas hydrophila ATCC7966 phaC) were introduced into the chromosome of two Pseudomonas strains: a native medium-chain-length 3-polyhydroxyalkanoate (PHA_MCL) producer (Pseudomonas sp. LFM046) and a UV-induced mutant strain unable to produce PHA (Pseudomonas sp. LFM461). We reported for the first time the insertion of a chromosomal copy of phaC using the transposon system mini-Tn7. Stable antibiotic marker-free and plasmid-free recombinants were obtained. Subsequently, P(3HB-co-3HA_MCL) was produced by these recombinants using glucose as the sole carbon source, without the need for co-substrates and under antibiotic-free conditions. A recombinant harboring A. hydrophila phaC produced a terpolyester composed of 84.2 mol% of 3-hydroxybutyrate, 6.3 mol% of 3-hydroxyhexanoate, and 9.5 mol% of 3-hydroxydecanoate from only glucose. Hence, we were successful in increasing the industrial potential of Pseudomonas sp. LFM461 strain by producing PHA copolymers containing 3HB and 3HA_MCL using an unrelated carbon source, for the first time in a plasmid- and antibiotic-free bioprocess.

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Section: Introductionmentioning

confidence: 83%

Production of Polyhydroxyalkanoates Copolymers by Recombinant Pseudomonas in Plasmid- and Antibiotic-Free Cultures

et al. 2018

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“…Considering those Y Xr/P values for E. coli and Pseudomonas, B. sacchari biomass composition presents lower phosphorus amounts. Also, as phosphorus is a significant component of nucleic acids, it should be noted that B. sacchari genome size is 7.2 Mb (Alexandrino et al, 2015), E. coli K-12 MG1655 genome size is 4.6 Mb (Blattner et al, 1997), and Pseudomonas LFM046 genome size is 6.0 Mb (Cardinali-Rezende et al, 2015). In addition, phospholipids are also important components synthesized from phosphorus.…”

Section: Growth Phasementioning

confidence: 99%

Investigating Nutrient Limitation Role on Improvement of Growth and Poly(3-Hydroxybutyrate) Accumulation by Burkholderia sacchari LMG 19450 From Xylose as the Sole Carbon Source

Oliveira-Filho

Silva

Macedo

et al. 2020

Front. Bioeng. Biotechnol.

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Burkholderia sacchari LMG19450, a non-model organism and a promising microbial platform, was studied to determine nutrient limitation impact on poly(3-hydroxybutyrate) [P(3HB)] production and bacterial growth from xylose, a major hemicellulosic residue. Nitrogen and phosphorus limitations have been studied in a number of cases to enhance PHA accumulation, but not combining xylose and B. sacchari. Within this strategy, it was sought to understand how to control PHA production and even modulate monomer composition. Nitrogen-limited and phosphorus-limited fed-batch experiments in bioreactors were performed to evaluate each one's influence on cell growth and poly(3-hydroxybutyrate) production. The mineral medium composition was defined based on yields calculated from typical results so that nitrogen was available during phosphorus limitation and residual phosphorus was available when limiting nitrogen. Sets of experiments were performed so as to promote cell growth in the first stage (supplied with initial xylose 15 g/L), followed by an accumulation phase, where N or P was the limiting nutrient when xylose was fed in pulses to avoid concentrations lower than 5 g/L. N-limited fed-batch specific cell growth (around 0.19 1/h) and substrate consumption (around 0.24 1/h) rates were higher when compared to phosphorus-limited ones. Xylose to PHA yield was similar in both conditions [0.37 g P(3HB) /g xyl ]. We also described pst gene cluster in B. sacchari, responsible for high-affinity phosphate uptake. Obtained phosphorus to biomass yields might evidence polyphosphate accumulation. Results were compared with studies with B. sacchari and other PHA-producing microorganisms. Since it is the first report of the mentioned kinetic parameters for LMG 19450 growing on xylose solely, our results open exciting perspectives to develop an efficient bioprocess strategy with increased P(3HB) production from xylose or xylose-rich substrates.

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“…Estas modificações se basearam em dados obtidos do draft do genoma de Pseudomonas sp. LFM046 recentemente publicado, além de outros estudos da literatura(Cardinali-Rezende et al, 2015;…”

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Avaliação da expressão de genes da via de Weimberg sobre o catabolismo de xilose na linhagem produtora de PHAMCL Pseudomonas sp. LFM046.

Sarmiento¹

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Draft Genome Sequence of Pseudomonas sp. Strain LFM046, a Producer of Medium-Chain-Length Polyhydroxyalkanoate

Cited by 9 publications

References 7 publications

Production of Polyhydroxyalkanoates Copolymers by Recombinant <b><i>Pseudomonas</i></b> in Plasmid- and Antibiotic-Free Cultures

Production of Polyhydroxyalkanoates Copolymers by Recombinant <b><i>Pseudomonas</i></b> in Plasmid- and Antibiotic-Free Cultures

Investigating Nutrient Limitation Role on Improvement of Growth and Poly(3-Hydroxybutyrate) Accumulation by Burkholderia sacchari LMG 19450 From Xylose as the Sole Carbon Source

Avaliação da expressão de genes da via de Weimberg sobre o catabolismo de xilose na linhagem produtora de PHA<sub>MCL </sub><i>Pseudomonas sp.</i> LFM046.

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