Draft Genome Sequence of
            <i>Metschnikowia australis</i>
            Strain UFMG-CM-Y6158, an Extremophile Marine Yeast Endemic to Antarctica

Batista, Thiago Mafra; Hilário, Heron O.; Moreira, Rennan Garcias; Furtado, Carolina; Godinho, Valéria M.; Rosa, Luiz Henrique; Franco, Glória Regina; Rosa, Carlos A.

doi:10.1128/genomea.00328-17

Cited by 8 publications

(3 citation statements)

References 14 publications

(12 reference statements)

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“…This meant that lack of C 18:2 fatty acid only could lead to the reduced growth rate of the yeast cells grown at 5°C and 25°C may by slowing down the cell division at 5°C and 25°C, but not affect cell growth of the mutant at 15°C. (Batista et al 2017). Therefore, the genome of M. australis strain W7-5 used in this study was similar to that of M. australis strain UFMG-CM-Y6158.…”

Section: Resultsmentioning

confidence: 81%

“…It has been reported that the assembled draft of M. australis strain UFMG-CM-Y6158 isolated from a marine macroalgae, Acrosiphonia arcta (Chlorophyta), collected in Admiralty Bay of King George Island in Keller Peninsula, Antarctica consisted of 14.3 Mbp over 160 contigs (> 505 bp) with a G + C content of 47.2%. The longest contig was 1,116,518 bp long, and the N50 contig length was 542,232 bp in M. australis strain UFMG-CM-Y6158(Batista et al 2017). M. australis strain UFMG-CM-Y6158 has 4,442 protein-coding genes…”

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confidence: 99%

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Genome-Wide Editing Provides Insights into Role of Unsaturated Fatty Acids in Low Temperature Growth of the Psychrophilic Yeast Metschnikowia australis W7-5

Wei

Zhang

Chi

et al. 2022

Preprint

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It has been confirmed that Fad12 (∆12 fatty acid desaturase) and Fad15 (∆15 fatty acid desaturase) were responsible for the synthesis of linoleic acid (C18:2, ∆9,12) and linolenic acid (C18:3, ∆9,12,15), respectively. In order to know their function in cold growth of the psychrophilic yeast Metschnikowia australis W7-5, the FAD12 gene and FAD15 gene were deleted, respectively. The intracellular linoleic acid percentage of the obtained Δfad12 mutant was decreased from 27.1–1.5% while the percentage of C18:1 fatty acid was increased from 28.3–55.7%. The growth rate of the Δfad12 mutant was significantly reduced when it was cultured at 5 ℃ and 25 ℃ compared with that of the wild type strain W7-5 under the same conditions. But at 15°C, the mutant grew as well as its wild type strain W7-5. Although C18:3 fatty acid of the Δfad15 strain were not detected, there was no significant difference between the growth of Δfad15 and that of the W7-5 strain at different temperatures. After the FAD12 gene was supplemented, the growth at different temperatures and intracellular fatty acid compositions of the supplementing strain were restored compared to those of the strain W7-5. These results suggested that only linoleic acid synthesized by the psychrophilic yeast, not linolenic acid synthesized, played an important role in adaptation to low temperature (5°C) and high temperature (25°C) of the psychrophilic yeast, Meanwhile, it was found that cell wall in the mutant Δfad12 grown at 5 and 25°C was also negatively affected after the mutant could not synthesize C18:2 fatty acids. This caused the reduced cell growth rate of the mutant Δfad12 grown at 5 and 25°C.

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Section: Resultsmentioning

confidence: 81%

mentioning

confidence: 99%

Genome-Wide Editing Provides Insights into Role of Unsaturated Fatty Acids in Low Temperature Growth of the Psychrophilic Yeast Metschnikowia australis W7-5

Wei

Zhang

Chi

et al. 2022

Preprint

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“…To date, many of the Metschnikowia sp. yeast genomes have been successfully analyzed using various next-generation sequencing platforms, such as genome sequence of Metschnikowia fructicola ( Piombo et al 2018 ), draft genome of Metschnikowia australis ( Batista et al 2017 ), draft genome of Metschnikowia pulcherrima subclade, UCD127 ( Venkatesh et al 2018 ), etc. Here, we report the first genome assembly of M. persimmonesis KCTC 12991BP (KIOM G15050 strain).…”

Section: Introductionmentioning

confidence: 99%

The whole-genome sequence of the novel yeast species Metschnikowia persimmonesis isolated from medicinal plant Diospyros kaki Thunb

Rahmat

Park

Kang

2021

G3 Genes|Genomes|Genetics

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The new yeast Metschnikowia persimmonesis KCTC 12991BP (KIOM G15050 strain) exhibits strong antimicrobial activity against some pathogens. This activity may be related to the medicinal profile of secondary metabolites that could be found in the genome of this species. Therefore, to explore its future possibility of producing some beneficial activities, including medicinal ability, we report high quality whole-genome assembly of M. persimmonesis produced by PacBio RSII sequencer. The final draft assembly consisted of 16 scaffolds with GC content of 45.90% and comprised a fairly complete set (82.8%) of BUSCO result using Saccharomycetales lineage data set. The total length of the genome was 16.473 Mb, with a scaffold N50 of 1.982 Mb. Annotation of the M. persimmonesis genome revealed presence of 7,029 genes and 6,939 functionally annotated proteins. Based on the analysis of phylogenetic relationship and the average nucleotide identities (ANI), M. persimmonesis was proved to a novel species within the Metschnikowia genus. This finding is expected to significantly contribute to the discovery of high-value natural products from M. persimmonesis as well as for genome biology and evolution comparative analysis within Metschnikowia species.

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Genomics of Antarctic Fungi: A New Frontier

Hilário

Batista

Franco

2019

Fungi of Antarctica

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Draft Genome Sequence of Metschnikowia australis Strain UFMG-CM-Y6158, an Extremophile Marine Yeast Endemic to Antarctica

Abstract: Here we report the draft genome sequence of Metschnikowia australis strain UFMG-CM-Y6158, a yeast endemic to Antarctica. We isolated the strain from the marine seaweed Acrosiphonia arcta (Chlorophyta). The genome is 14.3 Mb long and contains 4,442 predicted protein-coding genes.

Cited by 8 publications

References 14 publications

Genome-Wide Editing Provides Insights into Role of Unsaturated Fatty Acids in Low Temperature Growth of the Psychrophilic Yeast Metschnikowia australis W7-5

Genome-Wide Editing Provides Insights into Role of Unsaturated Fatty Acids in Low Temperature Growth of the Psychrophilic Yeast Metschnikowia australis W7-5

The whole-genome sequence of the novel yeast species Metschnikowia persimmonesis isolated from medicinal plant Diospyros kaki Thunb

Genomics of Antarctic Fungi: A New Frontier

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