Draft Genome Sequence of
            <i>Halomonas</i>
            sp. HG01, a Polyhydroxyalkanoate-Accumulating Strain Isolated from Peru

Cardinali-Rezende, Juliana; Nahat, Rafael A.T.P.S.; Moreno, César Wilber Guzmán; Farfán, Carmen Rosa Carreño; Silva, Luiziana Ferreira da; Taciro, Marilda Keico; Gomez, José Gregório Cabrera

doi:10.1128/genomea.01598-15

Cited by 6 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relative abundances of the bacteria at the genus level are shown in Figure S3 of the Supplementary Materials. As in previous studies, the dominance of Firmicutes and Bacteroidetes in digesters is frequently observed [ 34 ], as they are hydrolytic bacteria [ 33 , 35 , 36 ] responsible for the breakdown of polymeric substrates, such as proteins, lipids, and polysaccharides. Proteobacteria, known as acidogenic bacteria, was one of the dominant phyla in this study, in line with previous studies in AD [ 36 , 37 ].…”

Section: Resultsmentioning

confidence: 75%

Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio

Corbellini

Chen

Bellucci

et al. 2021

Archaea

View full text Add to dashboard Cite

The effect of the amount of hydrogen supplied for the in situ biological biogas upgrading was investigated by monitoring the process and evolution of the microbial community. Two parallel reactors, operated at 37°C for 211 days, were continuously fed with sewage sludge at a constant organic loading rate of 1.5 gCOD∙(L∙d)-1 and hydrogen (H2). The molar ratio of H2/CO2 was progressively increased from 0.5 : 1 to 7 : 1 to convert carbon dioxide (CO2) into biomethane via hydrogenotrophic methanogenesis. Changes in the biogas composition become statistically different above the stoichiometric H2/CO2 ratio (4 : 1). At a H2/CO2 ratio of 7 : 1, the methane content in the biogas reached 90%, without adversely affecting degradation of the organic matter. The possibility of selecting, adapting, and enriching the original biomass with target-oriented microorganisms able to biologically convert CO2 into methane was verified: high throughput sequencing of 16S rRNA gene revealed that hydrogenotrophic methanogens, belonging to Methanolinea and Methanobacterium genera, were dominant. Based on the outcomes of this study, further optimization and engineering of this process is feasible and needed as a means to boost energy recovery from sludge treatment.

show abstract

Section: Resultsmentioning

confidence: 75%

Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio

Corbellini

Chen

Bellucci

et al. 2021

Archaea

View full text Add to dashboard Cite

show abstract

“…Halomonas sp. HG03 was submitted to evolutionary engineering so a glycerol-consuming strain could be obtained (Moreno, 2015;Cardinali-Rezende et al, 2016). An efficient glycerol-consuming strain was also employed for PHB production experiments: Ralstonia eutropha PHB -4 LFM035-9 pBBR1MCS-2::phaPCJ, which was conveniently named as R. eutropha L359PCJ in this study.…”

Section: Microrganismsmentioning

confidence: 99%

Technical-Economical Approach for PHB Production by Ralstonia Eutropha Strain Using Concentrated Vinasse as Carbon Source and Other Biotechnological Applications

Silverio

Piccoli

Reis

et al. 2021

Preprint

View full text Add to dashboard Cite

The Brazilian ethanol industry is one of the most important in the global market, however these important industrial activities have been generating significant amounts of vinasse and its management has become costly for distilleries. In this study, the aim was to evaluate concentrated and in natura vinasse as basal culture media for biotechnological processes. Different bacteria and processes were assessed: L-threonine production by E. coli THR14, with glucose as carbon source; PHB production by halophilic strain Halomonas sp. HG03, with sucrose as carbon source; and PHB biosynthesis by R. eutropha L359PCJ, which used glycerol from vinasse as carbon source. Strains were evaluated firstly in shake flasks cultivations using vinasse-based media. E. coli THR14 had no statistical difference for biomass and L-threonine concentrations among control and vinasse-based treatments (up to 50% v v-1 of in natura vinasse). Halomonas sp. HG03 and R. eutropha L359PCJ were cultivated in mineral media diluted by in natura (50% and 75% v v-1) and concentrated (50% and 75% v v-1) vinasses. Higher vinasse concentrations resulted in higher cellular growth rather than PHB accumulation for both bacteria. In vinasse-based treatments, Halomonas sp. HG03 had PHB content between 19.6 – 75.2% and R. eutropha L359PCJ, 48.4 – 68.5%. 50% (v v-1) of concentrated vinasse was the most attractive condition for PHB production by both bacteria. Further experiments in CSTR bioreactors used this nutritional condition and R. eutropha L359PCJ had PHB content of 66.3%, concentrations of residual cell dry weight (rCDW) = 9.4 g L-1 and PHB = 18.6 g L-1, with YX/S = 0.16 g gGLYCEROL-1, YP/S = 0.32 g gGLYCEROL-1 and 0.25 gPHB Lh-1. Halomonas sp. HG03 had PHB content of 45.7%, rCDW = 9.8 g L-1, PHB = 8.3 g L-1 and YX/S = 0.18 g gSUCROSE-1, YP/S = 0.16 g gSUCROSE-1 and 0.12 gPHB Lh-1. Finally, cost reductions of PHB production by R. eutropha L359PCJ with concentrated vinasse-based medium were evaluated in silico by using SuperPro Designer. As a partial source of glycerol and other nutrients for PHB production by R. eutropha L359PCJ, vinasse reduced overall production costs by 13%. Simulated processes that used concentrated vinasse-based media combined with improvements of PHB productivity and higher cellular densities had production costs between US$ 3.9 – 7.5/kgPHB and 2.6 – 7.3 years of payback time.

show abstract

“…Dentro del grupo de bacterias halófilas y halotolerantes se encuentran especies del género Halomonas (Le Borgne et al, 2008), bacterias Gram negativas, heterotróficas, de rápido crecimiento y tolerantes a los ambientes salinos (Kim et al, 2013). Según varias investigaciones realizadas se aislaron especies de Halomonas en plantas marinas (Bibi, 2016), lagos salinos (Singh et al, 2018;Ming et al, 2020;Mamani et al, 2019), llanura costera (Koh et al, 2017), sedimento del Océano Índico, (Qiu et al, 2021), suelo salino (Li et al, 2022;Gan et al, 2018), piscina de minerales (Romano et al, 2019), desiertos (Hintersatz et al, 2023;Patel, & Thaker, 2015), reservorios de petróleo (Mnif et al, 2009), efluentes provenientes del procesamiento del olivo (Liebgott et al, 2008), efluentes provenientes del procesamiento de licor (Yang et al, 2010), minas de sal (Cardinali-Rezende et al, 2016), criaderos de Palaemon serratus "camarones" (Xue et al, 2018), y de Litopenaeus vannamei (Kasan et al, 2017). L. vannamei, conocido como "camarón blanco", es nativo de la costa oriental del Océano Pacifico, México (ciudad de Sonora) hasta Perú (ciudad de Tumbes), se encuentran en hábitats marinos tropicales.…”

Section: Introductionunclassified

Diversidad de especies nativas de Halomonas con capacidad nitrificante aisladas de criaderos de Litopenaeus vannamei

Pérez Tejeda,

Rojas Suárez,

Prado Chávarri

et al. 2023

Manglar

View full text Add to dashboard Cite

The objective of the research was to identify the species of the genus Halomonas, with nitrifying capacity, isolated from sediments of Litopenaeus vannamei farms from shrimp farms in Tumbes-Peru. From the sediment of 3 prawn farms at harvest time, a representative sample was obtained, to which a pre-enrichment was carried out in Marine Broth for 48 hours, then it was cultured in Marine Agar, until the observation of colonies characteristic of the genus Halomonas. The cultures obtained were identified phenotypically at the species level, to then evaluate their nitrifying capacity according to the Griess-llosvay methodology. As results, the isolation of 5 bacterial cultures was obtained, two identified as H. sulfidaeris and 3 as H. salina; All cultures showed the ability to reduce nitrate to nitrite by eliminating ammonium from the medium. It is concluded that in the sediment of Litopenaeus vannamei breeding sites located in Tumbes-Peru, the species H. sulfidaeris and H. salina are present, with nitrifying capacity, which allow the elimination of ammonium, which is developed as an effective option for the saline ammonium wastewater treatment.

show abstract

Draft Genome Sequence of Halomonas sp. HG01, a Polyhydroxyalkanoate-Accumulating Strain Isolated from Peru

Cited by 6 publications

References 2 publications

Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio

Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio

Technical-Economical Approach for PHB Production by Ralstonia Eutropha Strain Using Concentrated Vinasse as Carbon Source and Other Biotechnological Applications

Diversidad de especies nativas de Halomonas con capacidad nitrificante aisladas de criaderos de Litopenaeus vannamei

Contact Info

Product

Resources

About