Draft Genome Sequence of Exiguobacterium sp. Strain N5, Isolated from a Recreational Freshwater Kettle Lake in Ontario

Abstract

Exiguobacterium spp. are facultative anaerobic, Gram-positive, non-spore-forming bacilli, reported to tolerate extreme environments. Here, we report the draft genome sequence of Exiguobacterium sp. strain N5, isolated from a recreational freshwater lake.

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“…Bacterial colonies of differing morphologies were subcultured on TSA. Genomic DNA was extracted from a pure colony using the DNeasy blood and tissue kit (Qiagen, Hilden, Germany) and sequenced using the Illumina DNA library prep tagmentation kit and IDT for Illumina DNA/RNA unique dual (UD) indexes as previously described ( 5 ).…”

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“…Water samples were kept at 4° C and analyzed within 48 hours of collection. Samples were processed as described by Bryan et al, 2023 (23). Briefly, 1 mL of samples was serially diluted in 9 mL of lambda buffer (modified saline-magnesium buffer without gelatin) and plated onto tryptic soy agar (TSA).…”

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“…Briefly, DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). DNA libraries were prepared using the Illumina DNA Prep Tagmentation Kit and Integrated DNA Technologies for Illumina DNA/RNA unique dual indexes ( 5 , 6 ). Paired-end (2 × 150 bp) sequencing was performed on the Illumina MiniSeq system.…”