Draft Genome Sequence of
            <i>Arthrobacter</i>
            sp. Strain SPG23, a Hydrocarbon-Degrading and Plant Growth-Promoting Soil Bacterium

Gkorezis, Panagiotis; Bottos, Eric; Hamme, Jonathan D. Van; Thijs, Sofie; Rineau, François; Franzetti, Andrea; Balseiro-Romero, María; Weyens, Nele; Vangronsveld, Jaco

doi:10.1128/genomea.01517-15

Cited by 11 publications

(8 citation statements)

References 21 publications

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“…Both SPG23 and GK2 were isolated from poplar rhizospheric soil, and PF1 is an endophyte isolated from poplar tissues. Genes for plant growth-promoting characteristics were present spread throughout the genome, corroborating results from phenotypic tests: PF1 [20] and GK2 (Panagiotis Gkorezis, personal communication, February 2017) have the capacities for 1-aminocyclopropane-1-carboxylate deaminase activity, auxin biosynthesis, siderophore production, and inorganic phosphorous solubilization; and SPG23, for nitrogen fixation, and also for siderophore production and inorganic phosphorous solubilization and uptake [21]. These strains presented alkane-degrading genes spread across the genome (principally genes for the alkane hydroxylase system), as well as other genes involved in the degradation of aromatic hydrocarbons (benzene or naphthalene).…”

Section: Bacterial Strainssupporting

confidence: 67%

“…HZ01, which degraded up to 90% diesel in 10 days of incubation in minimal salt medium with 2% (w/v) of evaporated diesel oil. PF1, SPG23 and GK2 strains were subjected to whole genome sequencing and genes which are known to be involved in the degradation of alkanes, benzene and naphthalene and biofilm formation were present [20,21], thus confirming their ability for diesel-degradation.…”

Section: Discussionmentioning

confidence: 93%

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Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

Balseiro-Romero

Gkorezis

Kidd

et al. 2017

International Journal of Phytoremediation

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Bioremediation of polluted soils is a promising technique with low environmental impact, which uses soil organisms to degrade soil contaminants. In this study, 19 bacterial strains isolated from a diesel-contaminated soil were screened for their diesel-degrading potential, biosurfactant (BS) production, and biofilm formation abilities, all desirable characteristics when selecting strains for re-inoculation into hydrocarbon-contaminated soils. Diesel-degradation rates were determined in vitro in minimal medium with diesel as the sole carbon source. The capacity to degrade diesel range organics (DROs) of strains SPG23 (Arthobacter sp.) and PF1 (Acinetobacter oleivorans) reached 17-26% of total DROs after 10 days, and 90% for strain GK2 (Acinetobacter calcoaceticus). The amount and rate of alkane degradation decreased significantly with increasing carbon number for strains SPG23 and PF1. Strain GK2, which produced BSs and biofilms, exhibited a greater extent, and faster rate of alkane degradation compared to SPG23 and PF1. Based on the outcomes of degradation experiments, in addition to BS production, biofilm formation capacities, and previous genome characterizations, strain GK2 is a promising candidate for microbial-assisted phytoremediation of diesel-contaminated soils. These results are of particular interest to select suitable strains for bioremediation, not only presenting high diesel-degradation rates, but also other characteristics which could improve rhizosphere colonization.

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Section: Bacterial Strainssupporting

confidence: 67%

Section: Discussionmentioning

confidence: 93%

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

Balseiro-Romero

Gkorezis

Kidd

et al. 2017

International Journal of Phytoremediation

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“…Arthrobacter has also been found to degrade a wide variety of compounds, including aromatic molecules, organochloride, pesticides, etc. 20 . In addition, several species of this genus are desiccation tolerant 21 , 22 .…”

Section: Introductionmentioning

confidence: 99%

The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils

Fernández-González

Martínez‐Hidalgo

Cobo‐Díaz

et al. 2017

Sci Rep

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After a forest wildfire, the microbial communities have a transient alteration in their composition. The role of the soil microbial community in the recovery of an ecosystem following such an event remains poorly understood. Thus, it is necessary to understand the plant-microbe interactions that occur in burned soils. By high-throughput sequencing, we identified the main bacterial taxa of burnt holm-oak rhizosphere, then we obtained an isolate collection of the most abundant genus and its growth promoting activities were characterised. 16S rRNA amplicon sequencing showed that the genus Arthrobacter comprised more than 21% of the total community. 55 Arthrobacter strains were isolated and characterized using RAPDs and sequencing of the almost complete 16S rRNA gene. Our results indicate that isolated Arthrobacter strains present a very high genetic diversity, and they could play an important ecological role in interaction with the host plant by enhancing aerial growth. Most of the selected strains exhibited a great ability to degrade organic polymers in vitro as well as possibly presenting a direct mechanism for plant growth promotion. All the above data suggests that Arthrobacter can be considered as an excellent PGP rhizobacterium that may play an important role in the recovery of burned holm-oak forests.

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“…FB24 from soil in Seymour, Indiana (53) and Arthrobacter sp. SPG23 from contaminated soil at the Ford Motor Company site in Genk, Belgium (54), clustered together in the lineage 1. Strains of Arthrobacter sp.…”

Section: Resultsmentioning

confidence: 99%

Comparative genomic analysis reveals a monophyletic cold adapted Arthrobacter cluster from polar and alpine regions

Shen

Liu

et al. 2019

Preprint

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22Decrease in the frequency of arginine and increase in lysine are the trends that have 23 been identified in the genomes of cold adapted bacteria. However, some cold adapted 24 taxa show only limited or no detectable changes in the frequencies of amino acid 25 composition. Here, we examined Arthrobacter spp. genomes from a wide range of 26 environments on whether the genomic adaptations can be conclusively identified 27 across genomes of taxa from polar and alpine regions. Phylogenetic analysis with a 28 concatenated alignment of 119 orthologous proteins revealed a monophyletic 29 clustering of seven polar and alpine isolated strains. Significant changes in amino acid 30 composition related to cold adaptation were exclusive to seven of the twenty-nine 31 strains from polar and alpine regions. Analysis of significant indicator genes and cold 32 shock genes also revealed that clear differences could only be detected in the same 33 seven strains. These unique characteristics may result from a vast exchange of 34 genome content at the node leading to the monophyletic cold adapted Arthrobacter 35 cluster predicted by the birth-and-death model. We then experimentally validated that 36 strains with significant changes in amino acid composition have a better capacity to 37 grow at low temperature than the mesophilic strains. 38 Importance 39 Acquisition of novel traits through horizontal gene transfer at the early divergence of 40 the monophyletic cluster may accelerate their adaptation to low temperature. Our 41 study reached a clear relationship between adaptation to cold and genomic features 42 and would advanced in understanding the ambiguous results produced by the previous 43 studies on genomic adaption to cold temperature. 44 regions 46 47 65 validated by analysis the structural, kinetic and microcalorimetric of cold adapted 66 enzymes, e.g. aminopeptidase, β-lactamases, and dienelactone hydrolase (GaDlh) 67 (12-14).68 69 Comparative genomic analyses to examine differences in amino acid composition 70 toward cold adaptation came through the study of two methanogenic Archaea, 71 Methanogenium frigidum and Methanococcoides burtonii from Ace Lake, Antarctica 72 (15). Proteins from these cold-adapted Archaea were characterized by a higher 73 proportion of non-charged polar amino acids, such as glutamine and threonine, and a 74 lower proportion of hydrophobic amino acids, particularly leucine (15). The amino 75 acid shifts toward increased enzyme flexibility, which confers catalytic efficiency and 76 contribute to cold adaptation can be identified in genomes of Psychromonas 77 ingrahamii 37, Exiguobacterium sibiricum 255-15, Psychrobacter arcticus 273-4, 78 Shewanella spp. and Glaciecola spp. (16-19). However, these changes were limited in 79 Colwellia psychrerythraea 34H, Planococcus halocryophilus Or1, Rhodococcus sp. 80 JG3, Arthrobacter spp., Actinotalea sp. KRMCY2, Polaromonas sp. Eur3 1.2.1, 81 Paenisporosarcina sp. Eur1 9.01.10, Methylobacterium sp. AL-11 and Kocuria sp. 82 KROCY2 (13, 20-24). Even, no changes ...

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Draft Genome Sequence of Arthrobacter sp. Strain SPG23, a Hydrocarbon-Degrading and Plant Growth-Promoting Soil Bacterium

Cited by 11 publications

References 21 publications

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils

Comparative genomic analysis reveals a monophyletic cold adapted Arthrobacter cluster from polar and alpine regions

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