Draft Genome Sequence of Halomonas sp. KHS3, a Polyaromatic Hydrocarbon-Chemotactic Strain

Gasperotti, Ana Florencia; Studdert, Claudia A.; Revale, Santiago; Seitz, María Karina Herrera

doi:10.1128/genomea.00020-15

Cited by 13 publications

(17 citation statements)

References 7 publications

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“…The Vibrionales OTU identified were assigned to the genus Pseudoalteromonas or to the family Pseudoalteromonadaceae , and were detected only in the Apapa sediment. Other known PAH/hydrocarbon degraders were Alcanivorox ( Luan et al, 2014 ; Campos et al, 2015 ) and Halomonas ( Gasperotti et al, 2015 ; Gutierrez et al, 2015 ). In the Eledu sediment, there was only one Oceanospirillales OTU ( Oleibacter ) and in the Ofin site there were no OTU identified as Oceanospirillales .…”

Section: Resultsmentioning

confidence: 99%

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

et al. 2016

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Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa’s largest estuarine ecosystems, and is impacted by hydrocarbon pollutants and other industrial and municipal wastes. The goal of this study was to elucidate microbial community structure in Lagos lagoon sediments to identify groups that may be adversely affected by pollution, and those that may serve as degraders of environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Sediment samples were collected from sites that ranged in types and levels of anthropogenic impacts. The sediments were characterized for a range of physicochemical properties, and microbial community structure was determined by Illumina sequencing of the 16S rRNA genes. Microbial diversity (species richness and evenness) in the Apapa and Eledu sediments was reduced compared to that of the Ofin site, and communities of both of the former two were dominated by a single operational taxonomic unit (OTU) assigned to the family Helicobacteraceae (Epsilonproteobacteria). In the Ofin community, Epsilonproteobacteria were minor constituents, while the major groups were Cyanobacteria, Bacteroidetes, and Firmicutes, which were all minor in the Apapa and Eledu sediments. Sediment oxygen demand (SOD), a broad indicator of contamination, was identified by multivariate analyses as strongly correlated with variation in alpha diversity. Environmental variables that explained beta diversity patterns included SOD, as well as levels of naphthalene, acenaphthylene, cobalt, cadmium, total organic matter, or nitrate. Of 582 OTU identified, abundance of 167 was significantly correlated (false discovery rate q≤ 0.05) to environmental variables. The largest group of OTU correlated with PAH levels were PAH/hydrocarbon-degrading genera of the Oceanospirillales order (Gammaproteobacteria), which were most abundant in the hydrocarbon-contaminated Apapa sediment. Similar Oceanospirillales taxa are responsive to marine oil spills and thus may present a unifying theme in marine microbiology as bacteria adapted for degradation of high hydrocarbon loads, and may represent a potential means for intrinsic remediation in the case of the Lagos lagoon sediments.

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Section: Resultsmentioning

confidence: 99%

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

et al. 2016

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“…Though extensively studied in model organisms such as Escherichia coli and Pseudomonas spp. [19], little is known about these chemotactic bacterial systems in halophilic bacteria such as those of the genus Halomonas [25][26][27], which have, however, been studied in relation to Salicornia root colonization [63]. We assayed the motility of bacteria belonging to the genus Halomonas that were isolated from the halophyte rhizosphere.…”

Section: Discussionmentioning

confidence: 99%

“…Ling et al [23] have reported that organic acids in watermelon root exudates recruit Paenibacillus polymyxa SQR-21, while Rudrappa et al have described how malic acid in plant roots chemically attracts Bacillus subtilis FB17 [24]. However, little is known about the responses of halophilic bacteria to chemical and natural compounds or their chemotaxis machinery, with only a few recent studies describing the chemotaxis-mediated responses of H. anticariensis FP35 T to environmental pollutants [25] and analysing the genome of Halomonas strains to search for chemosensory genes and the chemosensory pathways within the genus Halomonas [26,27].…”

Section: Introductionmentioning

confidence: 99%

Effects of Halophyte Root Exudates and Their Components on Chemotaxis, Biofilm Formation and Colonization of the Halophilic Bacterium Halomonas Anticariensis FP35T

Sampedro

Pérez-Mendoza

Toral³

et al. 2020

Microorganisms

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Increase in soil salinity poses an enormous problem for agriculture and highlights the need for sustainable crop production solutions. Plant growth-promoting bacteria can be used to boost the growth of halophytes in saline soils. Salicornia is considered to be a promising salt-accumulating halophyte for capturing large amounts of carbon from the atmosphere. In addition, colonization and chemotaxis could play an important role in Salicornia-microbe interactions. In this study, the role of chemotaxis in the colonization of the halophilic siredophore-producing bacteria, Halomonas anticariensis FP35T, on Salicornia hispanica plants was investigated. The chemotactic response of FP35T to Salicornia root exudates showed optimum dependence at a salt concentration of 5 % NaCl (w/v). Oleanolic acid, the predominant compound in the exudates detected by HPLC and identified by UPLC-HRMS Q-TOF, acts as a chemoattractant. In vitro experiments demonstrated the enhanced positive effects of wild-type H. anticariensis strain FP35T on root length, shoot length, germination and the vigour index of S. hispanica. Furthermore, these positive effects partially depend on an active chemotaxis system, as the chemotaxis mutant H. anticariensis FP35 ΔcheA showed reduced plant growth promotion for all the parameters tested. Overall, our results suggest that chemotaxis responses to root exudates play an important role in interactions between Salicornia and halophilic bacteria, enhance their colonization and boost plant growth promotion. Preliminary results also indicate that root exudates have a positive impact on H. anticariensis FP35T biofilm formation under saline conditions, an effect which totally depends on the presence of the cheA gene.

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“…Further, the Oceanospirillales Alcanivorax borkumensis is a ubiquitous n-alkane degrading marine bacterium (Schneiker et al, 2006). Further, several Halomonadaceae are capable of growth on hydrocarbons (for example Gasperotti et al, 2015). However, whether the Halomonadaceae discussed here is capable of oxidizing CH 4 is unknown.…”

Section: Euphotic Zone Bacterioplankton Structure and Diversitymentioning

confidence: 95%

Methane and microbial dynamics in the Gulf of Mexico water column

et al. 2015

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In contrast to other oligotrophic water bodies the Gulf of Mexico (GOM) hosts an abundance of hydrocarbon seeps, which likely influences the microbial assemblages it hosts particularly regarding the availability of labile carbon in the aphotic GOM. The aphotic zone receives direct injection of seep methane (CH 4 ), but CH 4 from an unknown source has been reported at supersaturated concentrations relative to the atmosphere in the GOM photic zone. Here we used iTag sequencing of 16S rRNA genes to characterize GOM microbial communities and to relate changes in microbial community structure to the properties inherent to their oceanic province-seafloor to the photic zone, seep and non-seep. Along this trajectory water column communities were distinct in the euphotic zone compared to the mesopelagic and deep-sea. In the euphotic zone the relative abundance of a cyanobacterial species (Prochlorococcus) was significantly correlated with both CH 4 and chlorophyll a concentrations and was abundant in some deep-chlorophyll maximum (DCM) samples. The relative abundance of microorganisms related to known hydrocarbon degraders were also significantly correlated with CH 4 in the euphotic zone, but no canonical methanotrophs were observed. In the mesopelagic to the seafloor canonical methanotrophs were identified, but only a Marine Group II Euryarchaeota was significantly correlated with CH 4 . Overall, depth and the associated environmental conditions were the primary drivers in structuring microbial communities over the GOM water column. Further, CH 4 concentrations and relative microbial abundances covaried significantly from the seafloor to the photic zone in the GOM. The lack of a significant relationship between canonical methanotrophs and CH 4 in the aphotic zone, even when sampling at seep sites, may suggest methane-oxidation by unknown microorganisms. Similarly their absence in the CH 4 maximum and DCM suggested that CH 4 is either oxidized by unrecognized methanotrophs or escapes the CH 4 biofilter and fluxes to the atmosphere.

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Draft Genome Sequence of Halomonas sp. KHS3, a Polyaromatic Hydrocarbon-Chemotactic Strain

Cited by 13 publications

References 7 publications

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

Effects of Halophyte Root Exudates and Their Components on Chemotaxis, Biofilm Formation and Colonization of the Halophilic Bacterium Halomonas Anticariensis FP35T

Methane and microbial dynamics in the Gulf of Mexico water column

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