Draft genome sequence of Hahella sp. CR1 and its ability in producing cellulases for saccharifying agricultural biomass

Tan, Melvin Chun Yun; Zakaria, Muhammad Ramziuddin; Liew, Kok Jun; Chong, Chun Shiong

doi:10.1007/s00203-023-03617-6

Cited by 2 publications

(2 citation statements)

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“…As mentioned, similarities co-embodied in individual strains using nitrogen sources are commonly acknowledged for their positive effects on cellulase production, such as tryptone, peptone, and yeast extract [3,18,59,63], indicating a non-stringent demand on nitrogen sources. This study further emphasized the significance of moderate salinity in influencing factorial interactions that contributed to production, which caused less attention unless in studies with a focus on halotolerance [43,52]. The preference of such salinity (approximately 2% NaCl) could be partially attributed to the saline-abundant conditions in composting due to both animal excrement and marine waste.…”

Section: Path Of Steepest Ascent Designmentioning

confidence: 85%

“…The BLAST results declared that V7S33_05150 could be regarded as an endoglucanase (EC 3.2.1.4) with 100% identity, while V7S33_13785 belonged to β-glucosidase (EC 3.2.1.21) in strain Z2.6 with 99.57% identities. Notably, V7S33_05150 was detected with a carbohydrate-binding site, which may enhance carbohydrate catalytic abilities [14,52], and V7S33_13965 also contained a signal peptide, suggesting a possible extracellular role in hydrolyzing (1->4)beta-D-glucosidic linkages. These genes all exhibited similarities above 99.5% against sequences in other B. velezensis strains.…”

Section: Genome Features and Mining Of Potential Cellulasesmentioning

confidence: 99%

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Introduction of Cellulolytic Bacterium Bacillus velezensis Z2.6 and Its Cellulase Production Optimization

Cai,

Wang,

You

et al. 2024

Microorganisms

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Enzyme-production microorganisms typically occupy a dominant position in composting, where cellulolytic microorganisms actively engage in the breakdown of lignocellulose. Exploring strains with high yields of cellulose-degrading enzymes holds substantial significance for the industrial production of related enzymes and the advancement of clean bioenergy. This study was inclined to screen cellulolytic bacteria, conduct genome analysis, mine cellulase-related genes, and optimize cellulase production. The potential carboxymethylcellulose-hydrolyzing bacterial strain Z2.6 was isolated from the maturation phase of pig manure-based compost with algae residuals as the feedstock and identified as Bacillus velezensis. In the draft genome of strain Z2.6, 31 related cellulolytic genes were annotated by the CAZy database, and further validation by cloning documented the existence of an endo-1,4-β-D-glucanase (EC 3.2.1.4) belonging to the GH5 family and a β-glucosidase (EC 3.2.1.21) belonging to the GH1 family, which are predominant types of cellulases. Through the exploration of ten factors in fermentation medium with Plackett–Burman and Box–Behnken design methodologies, maximum cellulase activity was predicted to reach 2.98 U/mL theoretically. The optimal conditions achieving this response were determined as 1.09% CMC-Na, 2.30% salinity, and 1.23% tryptone. Validation under these specified conditions yielded a cellulose activity of 3.02 U/mL, demonstrating a 3.43-fold degree of optimization. In conclusion, this comprehensive study underscored the significant capabilities of strain Z2.6 in lignocellulolytic saccharification and its potentialities for future in-depth exploration in biomass conversion.

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