Draft Genome Sequence of Fructophilic Lactobacillus florum

Kim, Eun Bae; Tyler, Charlotte; Kopit, Lauren M.; Marco, Maria L.

doi:10.1128/genomea.00025-12

Cited by 11 publications

(7 citation statements)

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“…florum 2F (Kim et al . ) using the blastp algorithm provided by the National Center for Biotechnology Information (NCBI) (http://blast.ncbi.nlm.nih.gov). Mannitol 2‐dehydrogenase from Leuconostoc pseudomesenteroides ATCC 12291 (accession number ) (Hahn et al .…”

Section: Methodsmentioning

confidence: 99%

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Polyol production during heterofermentative growth of the plant isolate Lactobacillus florum 2F

et al. 2016

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Aims: This study examined the fermentative growth and polyol production of Lactobacillus florum and other plant-associated lactic acid bacteria (LAB). Methods and Results: Sugar consumption and end-product production were measured for Lact. florum 2F in the presence of fructose, glucose and both sugars combined. The genome of Lact. florum was examined for genes required for mannitol and erythritol biosynthesis. The capacity for other plantassociated LAB to synthesize polyols was also assessed. Conclusions: Lactobacillus florum exhibited higher growth rates and cell yields in the presence of both fructose and glucose. Lactobacillus florum 2F produced lactate, acetate and ethanol as well as erythritol and mannitol. Lactobacillus florum 2F synthesized mannitol during growth on fructose and erythritol during growth on glucose. Gene and protein homology searches identified a mannitol dehydrogenase in the Lact. florum 2F genome but not the genes responsible for erythritol biosynthesis. Lastly, we found that numerous other heterofermentative LAB species synthesize erythritol and/or mannitol. Significance and Impact of the Study: Lactobacillus florum is a recently identified, plant-associated, fructophilic LAB species. Our results show that Lact. florum growth rates and heterofermentation end-products differ depending on the sugar substrates present and growth yields can be improved when combinations of sugars are provided. Lactobacillus florum 2F produces erythritol and mannitol, two polyols that are relevant to foods and potentially also in plant environments. The capacity for polyol biosynthesis appears to be common among plant-associated, LAB species.

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Section: Methodsmentioning

confidence: 99%

“…florum 2F, a strain for which the genome sequence is known (Kim et al . ). Fermentative growth of Lact.…”

Section: Introductionmentioning

confidence: 97%

Polyol production during heterofermentative growth of the plant isolate Lactobacillus florum 2F

et al. 2016

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“…For example, L. florum is a FLAB with small genome sizes (e.g. L. florum strain 2F genome is 1.26 Mbp; Kim et al ., ). This species was first isolated from peony ( Paeonia suffruticosa ) and bietou ( Chrysanthemoides monilifera ) flowers and thus far has only been isolated from flowers, fruits and wine (Endo et al ., ; ; Kim et al ., ).…”

Section: Specialist Plant‐associated Labmentioning

confidence: 97%

Abundance, diversity and plant‐specific adaptations of plant‐associated lactic acid bacteria

Leveau

Marco

2019

Environ Microbiol Rep

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Summary Lactic acid bacteria (LAB) are essential for many fruit, vegetable and grain food and beverage fermentations. However, the numbers, diversity and plant‐specific adaptions of LAB found on plant tissues prior to the start of those fermentations are not well understood. When measured, these bacteria have been recovered from the aerial surfaces of plants in a range from <10 CFU g−1 to over 108.5 CFU g−1 of plant tissue and in lower quantities from the soil and rhizosphere. Plant‐associated LAB include well‐known generalist taxa such as Lactobacillus plantarum and Leuconostoc mesenteroides, which are essential for numerous food and beverage fermentations. Other plant‐associated LAB encompass specialist taxa such as Lactobacillus florum and Fructobacillus, many of which were discovered relatively recently and their significance on plants and in foods is not yet recognized. LAB recovered from plants possess the capacity to consume plant sugars, detoxify phenolic compounds and tolerate the numerous biotic and abiotic stresses common to plant surfaces. Although most generalist and some specialist LAB grow rapidly in food and beverages fermentations and can cause spoilage of fresh and fermented fruits and vegetables, the importance of living plants as habitats for these bacteria and LAB contributions to plant microbiomes remain to be shown.

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“…The low synthesis of ethanol is due to the absence of acetaldehyde dehydrogenase and a weak activity of alcohol dehydrogenase (7). The second group includes Lactobacillus florum and a few biotypes of Lactobacillus brevis (2,4,5,27). Facultative FLAB grow well on fructose and are able to grow on glucose at a delayed rate if electron acceptors are absent.…”

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confidence: 99%

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

Filannino

Cagno

Addante

et al. 2016

Appl Environ Microbiol

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Fructophilic lactic acid bacteria (FLAB) are strongly associated with the gastrointestinal tracts (GITs) of Apis mellifera L. worker bees due to the consumption of fructose as a major carbohydrate. Seventy-seven presumptive lactic acid bacteria (LAB) were isolated from GITs of healthy A. mellifera L. adults, which were collected from 5 different geographical locations of the Apulia region of Italy. Almost all of the isolates showed fructophilic tendencies: these isolates were identified as Lactobacillus kunkeei (69%) or Fructobacillus fructosus (31%). A high-throughput phenotypic microarray targeting 190 carbon sources was used to determine that 83 compounds were differentially consumed. Phenotyping grouped the strains into two clusters, reflecting growth performance. The utilization of phenolic acids, such as p-coumaric, caffeic, syringic, or gallic acids, as electron acceptors was investigated in fructose-based medium. Almost all FLAB strains showed tolerance to high phenolic acid concentrations. p-Coumaric acid and caffeic acid were consumed by all FLAB strains through reductases or decarboxylases. Syringic and gallic acids were partially metabolized. The data collected suggest that FLAB require external electron acceptors to regenerate NADH. The use of phenolic acids as external electron acceptors by the 4 FLAB showing the highest phenolic acid reductase activity was investigated in glucose-based medium supplemented with p-coumaric acid. Metabolic responses observed through a phenotypic microarray suggested that FLAB may use p-coumaric acid as an external electron acceptor, enhancing glucose dissimilation but less efficiently than other external acceptors such as fructose or pyruvic acid. Fructophilic lactic acid bacteria (FLAB), described only recently (1-9), belong to a special group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. These bacteria have been isolated from specific ecological fructose-rich niches such as flowers, fruits, and fermented food based-fruits and, only recently, from the gastrointestinal tracts (GITs) of several insects (e.g., bumblebees, honeybees, tropical fruit flies, and Camponotus ants), which have a fructose-based diet (4, 10, 11). Among these insects, social honeybees are those of greater interest because of their economic and ecological importance for honeybee products (e.g., propolis, royal jelly, honey, and pollen) and especially for crop pollination. Despite a global increase in the population of domesticated bees according to the FAO data (12), honeybees are facing growing adversity. Localized declines in bee populations have occurred in many European countries (13-15). To understand and to prevent the decrease, several studies have been addressed to investigate the symbiotic and pathogenic microbial interactions (16-19). The observations made as of to date indicate the honeybees' GITs harbor a core microbiota dissimilar to those of other animals, including humans (16)(17)(18)(19)(20)(21)(22)(23)(24). FLAB are strongly as...

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Draft Genome Sequence of Fructophilic Lactobacillus florum

Abstract: Herein we report the first genome sequence for Lactobacillus florum. L. florum 2F was isolated from Valencia orange leaves and is fructophilic, like other strains of this species. The draft genome of L. florum 2F contains 1,261,842 bp with a G+C content of 41.5% in 46 contigs (≥500 bp).

Cited by 11 publications

References 9 publications

Polyol production during heterofermentative growth of the plant isolate Lactobacillus florum 2F

Polyol production during heterofermentative growth of the plant isolate Lactobacillus florum 2F

Abundance, diversity and plant‐specific adaptations of plant‐associated lactic acid bacteria

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

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