Draft Genome Sequence of Escherichia coli Strain Tj, Isolated from the Varzob River in Tajikistan

Abstract

The 4.6-Mbp draft genome sequence of Escherichia coli strain Tj, isolated from the Varzob River in Tajikistan, is presented. This strain possesses four prophage elements related to Shigella phage SfV, E. coli O157:H7-specific phage ϕV10, lambdoid phage HK225, and coliphage Ayreon. It contains a gene encoding a hemolysin E toxin.

Draft Genome Sequence of Agrobacterium radiobacter Strain MD22b, Isolated from a Grape Plant in Tajikistan

“…DNA was extracted from cells cultivated in LB for 24 h at 30°C with shaking using the GenElute bacterial genomic DNA kit (Sigma-Aldrich). Sequencing of the 16S rRNA gene using the primer combination 5′- AGRGTTTGATYHTGGCTCAG -3′ (27f_mod) and 5′- TASGGHTACCTTGTTACGACTT -3′ (1492r_mod) ( 4 ) as previously described ( 5 ) yielded a 1,275-nucleotide sequence identical to that of A. radiobacter strain ATCC 23308 (GenBank accession number MT534521.1 ) using BLASTn v. 2.13.0 searches against the GenBank v. 252 nonredundant nucleotide database ( https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome ). For genome sequencing by Eurofins Genomics, a NEBNext Ultra II DNA preparation kit was used, and Illumina NovaSeq 6000 S2 paired-end genomic sequencing was performed with a read length of 2 × 151 bp, resulting in 10,125,972 reads with a Phred score of ≥28 and a total of 1,518,897,000 sequenced bases.…”