Draft Genome Sequence of Erythromycin-Resistant Streptococcus gallolyticus subsp. gallolyticus NTS 31106099 Isolated from a Patient with Infective Endocarditis and Colorectal Cancer

Abstract

Streptococcus gallolyticus subsp. gallolyticus is known for its close association with infective endocarditis and colorectal cancer in humans. Here, we report the draft genome sequence of highly erythromycin-resistant strain NTS 31106099 isolated from a patient with infective endocarditis and colorectal cancer.

Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus

“…Recently, we reported the draft genome of multiresistant Sgg isolate NTS31106099 which was recovered in 2011 at Nantes University Hospital, France from positive blood culture of a patient with IE and CRC. 11 During routine AST, NTS31106099 displayed susceptibility to ampicillin (MIC " 0.125 mg/L) and gentamicin (MIC " 4 mg/L) as well as resistance to tetracyclines (.256 mg/L), aminoglycosides [kanamycin (.256 mg/L), streptomycin (.256 mg/L)] and macrolides [erythromycin (.256 mg/L), clindamycin (.256 mg/L)].…”

“…WGS is explained elsewhere. 11,12 Comparative analysis software and visualization is described at the end of the first Supplementary data file.…”

The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity of Streptococcus bovis/Streptococcus equinus Complex Members

“…The pangenome is increasing at a high rate entailing numerous unique genes for each new strain added (Hinse et al, 2011b ). Nearly all SBSEC genomes reveal horizontal gene transfer relating to general carbohydrate metabolism, capsular polysaccharides, antimicrobial resistance or tannase as the key discriminator of SGG (Rusniok et al, 2010 ; Hinse et al, 2011b ; Lin et al, 2011b ; Jans et al, 2013 ; Papadimitriou et al, 2014 ; Kambarev and Caté, 2015 ; Grimm et al, 2017a ; Kambarev et al, 2017 ). Natural competence operons and pseudopilus identified in SII and SGG likely contribute to the success of horizontal gene transfer and thus to niche adaptation (Rusniok et al, 2010 ; Lin et al, 2011b ; Morrison et al, 2013 ; Jans et al, 2016 ).…”

Draft Genome Sequences of Two Highly Erythromycin-Resistant Streptococcus gallolyticus subsp. gallolyticus Isolates Containing a Novel Tn 916 -Like Element, Tn 6331

“…Recently, we reported the draft genome sequence of highly erythromycin-resistant clinical isolate S. gallolyticus subsp. gallolyticus NTS31106099 ( 16 ). It was found to contain previously unknown putative Tn 916 -like conjugative transposon, Tn 6263 , which harbors an aminoglycoside/macrolide resistance cluster [ aph(3′)-III → ant(6)-Ia → ermB ].…”

“…Cultures and genomic DNA were prepared as described elsewhere ( 16 ). Genome fragmentation was performed with Bioruptor Standard (Diagenode).…”