Draft Genome Sequence of Entomopathogenic Brevibacillus laterosporus Strain Lak 1210, an Alkaliphilic Chitin Degrader

Prasanna, Lakshmi; Moharana, Tushar Ranjan; Nizamuddin, Sheikh; Arravapalli, Veerabhadra Rao; Kumaraswamy, Thangaraj; Eijsink, Vincent G. H.; Nalam, Madhusudhana Rao

doi:10.1128/genomea.01251-17

Cited by 4 publications

(1 citation statement)

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“…laterosporus strains have been sequenced and their genomes deposited in Genbank. These include LMG 15441 (under accession number AFRV00000000, [34]), GI-9 (under accession numbers CAGD01000001 to CAGD01000061, [35]), B9 (under accession numbers CP011074-CP011076, [36]), Lak 1210 (under accession number NDIP00000000, [37]), OSY-I 1 (under accession number NOLX00000000, [38]), DSM25 (accession number ARFS00000000), ZQ2, UBA5783, DSM25, PE36 (accession number NZ_AXBT00000000.1) and Uniss_18.…”

Section: Introductionmentioning

confidence: 99%

Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials

et al. 2019

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Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative ( Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive ( Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37°C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S . aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.

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