Draft genome sequence of Enterococcus faecium E86, a strain producing broad-spectrum antimicrobial peptides: Description of a novel bacteriocin immunity protein and a novel sequence type

Farias, Felipe Miceli de; Francisco, Márcia Silva; Santos, Ilana Nascimento de Sousa; Marques-Bastos, Selda Loase Salustiano; Miguel, Marco Antônio Lemos; Albano, Rodolpho Mattos; Bastos, Maria do Carmo de Freire

doi:10.1016/j.jgar.2019.04.004

Cited by 5 publications

(6 citation statements)

References 7 publications

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“…petauri LZys1 was involved in coding bacteriocin immunity protein ( GIG89_RS02705 ， GIG89_RS03460 ). Besides, Miceli [48] found a novel bacteriocin immunity protein (EntiTW21) and a novel multilocus sequence type (ST1500), which may be employed in the food industry as a biopreservative against L. monocytogenes .…”

Section: Discussionmentioning

confidence: 99%

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Ouyang

Zhang

Wang

et al. 2021

Journal of Medical Microbiology

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Introduction. Lactococcus petauri LZys1 ( L. petauri LZys1) is a type of lactic acid bacteria (LAB), which was initially isolated from healthy human gut. Hypothesis/Gap Statement. It was previously anticipated that L. petauri LZys1 has potential characteristics of probiotic properties. The genetic structure and the regulation functions of L. petauri LZys1 need to be better revealed. Aim. The aim of this study was to detect the probiotic properties L. petauri LZys1 and to reveal the genome information related to its genetic adaptation and probiotic profiles. Methodology. Multiple in vitro experiments were carried out to evaluate its lactic acid-producing ability, resistance to pathogenic bacterial strains, auto-aggregation and co-aggregation ability, and so on. Additionally, complete genome sequencing, gene annotation, and probiotic associated gene analysis were performed. Results. The complete genome of L. petauri LZys1 comprised of 1 985 765 bp, with a DNA G+C content of 38.07 %, containing 50 tRNA, seven rRNA, and four sRNA. A total of 1931 genes were classified into six functional categories by Kyoto Encyclopaedia of Genes and Genomes (KEGG) database. The neighbour-joining phylogeny tree based on the whole genome of L. petauri LZys1 and other probiotics demonstrated that L. petauri LZys1 has a significant similarity to Lactococcus garvieae . The functional genes were detected to expound the molecular mechanism and biochemical processes of its potential probiotic properties, such as atpB gene. Conclusion. All the results described in this study, together with relevant information previously reported, made L. prtauri LZys1 a very interesting potential strain to be considered as a prominent candidate for probiotic use.

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Section: Discussionmentioning

confidence: 99%

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Ouyang

Zhang

Wang

et al. 2021

Journal of Medical Microbiology

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“…Cytolysin has also been greatly associated with clinical E. faecalis, although not exclusively, being associated with a hemolytic phenotype, and a higher virulence in animal models [45]. Different studies also confirmed a higher occurrence of Bac32 and Bac43 among clinical VREfm/VSEfm than among nonclinical E. faecium isolates, with Bac43 being exclusively found in clinical VREfm [55,63,80]. A study performing a detailed location of plasmid and bacteriocin genes in clonally diverse outbreak VREfm from different countries and clinical VSEfm from Spain described a high prevalence of EntA, but Bac32, Bac43, EntB, and EntP rates were variable and detected at lower rates [56].…”

Section: Diversity Of Enterocins In Clinical Enterococcimentioning

confidence: 96%

“…EntP has been identified either on the chromosome or plasmids of different E. faecium strains isolated from foodstuffs or clinical isolates (Table S1) [61][62][63]. It has a wide antimicrobial spectrum that includes foodborne pathogens, such as L. monocytogenes, Clostridium botulinum, Clostridium perfringens, and Staphylococcus aureus, along with spoilage bacteria Staphylococcus carnosus, Clostridium sporogenes, E. faecalis, and Propionibacterium species, and even VREfm strains (Table S1) [61,63]. A broad antimicrobial spectrum alongside other characteristics, such as thermal resistance, activity in a wide range of pH values, and sensitivity to proteolytic enzymes [61], make this enterocin a potential food preservative.…”

Section: Class Iia-the Pediocin-like Bacteriocinsmentioning

confidence: 99%

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

et al. 2021

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Enterococcus spp. are one of the most frequent producers of bacteriocins (enterocins), which provides them with an advantage to compete in their natural environment, which is the gut of humans and many animals. The enterocins’ activity against microorganisms from different phylogenetic groups has raised interest in Enterococcus spp. in different contexts throughout the last decades, especially in the food industry. Nevertheless, some species can also cause opportunistic life-threatening infections and are frequently multidrug-resistant (MDR). Vancomycin-resistant Enterococcus (VRE), in particular, are an ongoing global challenge given the lack of therapeutic options. In this scenario, bacteriocins can offer a potential solution to this persistent threat, either alone or in combination with other antimicrobials. There are a handful of studies that demonstrate the advantages and applications of bacteriocins, especially against VRE. The purpose of this review is to present a current standpoint about the dual role of Enterococcus spp., from important producers to targets needed to be controlled, and the crucial role that enterocins may have in the expansion of enterococcal populations. Classification and distribution of enterocins, the current knowledge about the bacteriocinome of clinical enterococci, and the challenges of bacteriocin use in the fight against VRE infections are particularly detailed.

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“…EntP has been identified in both chromosomes and plasmids across various E. faecium strains isolated from food sources and clinical settings [98][99][100]. While its antimicrobial spectrum is wide, encompassing foodborne pathogens and spoilage bacteria, its presence in clinical isolates, albeit at low rates, suggests a potential role in enterococcal virulence or colonization within human hosts [30,31].…”

Section: Bacteriocinsmentioning

confidence: 99%

“…While its antimicrobial spectrum is wide, encompassing foodborne pathogens and spoilage bacteria, its presence in clinical isolates, albeit at low rates, suggests a potential role in enterococcal virulence or colonization within human hosts [30,31]. Studies have shown a higher occurrence of EntP and other bacteriocins among clinical vancomycin-resistant E. faecium (VREfm)/VSEfm strains compared to non-clinical isolates, indicating their relevance in clinical settings [95,98]. In addition, Farias et al tested the antimicrobial activity of EntP against 14 vanA-positive E. faecium and E. faecalis strains as a result of all the strains tested being susceptible to the bacteriocin, therefore highlighting their potential as therapeutic agents against antibiotic-resistant pathogens [101].…”

Section: Bacteriocinsmentioning

confidence: 99%

Virulence Determinants of Enterococcus Faecium

Reissier,

Penven,

Michaux

et al. 2024

Infectious Diseases

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Enterococcus faecium, a member of the human gut microbiota, has emerged as a notable opportunistic pathogen, contributing to a diverse range of hospital-acquired infections. Its capacity to thrive in various anatomical sites and initiate infections is attributed to an elaborate suite of virulence determinants. Prominent among these are cell surface components and pili structures, which facilitate initial adhesion and subsequent biofilm formation. Additionally, temperature-regulated gene expression augments virulence by enhancing adherence and biofilm formation. E. faecium also employs sophisticated mechanisms to modulate host immune responses, including hindering leukocyte killing through membrane structures like lipoteichoic acids and capsular polysaccharides. Bacteriocins confer a competitive edge by inhibiting competing bacteria, while global regulators orchestrate biofilm formation and stress responses. The stringent response further enhances adaptation to stress conditions. Understanding these virulence factors is paramount for unraveling the intricacies of E. faecium infections and devising effective therapeutic strategies.

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Draft genome sequence of Enterococcus faecium E86, a strain producing broad-spectrum antimicrobial peptides: Description of a novel bacteriocin immunity protein and a novel sequence type

Cited by 5 publications

References 7 publications

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

Virulence Determinants of Enterococcus Faecium

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