Draft Genome Sequence of Coxiella burnetii Strain Cb196, an Agent of Endocarditis in Saudi Arabia

D'Amato, Felicetta; Robert, Caroline; Azhar, Esam I.; Fournier, Pierre‐Edouard; Raoult, Didier

doi:10.1128/genomea.01180-14

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…In both papers, MLVA genotyping revealed further discrimination of the MST types in two or three other subtypes [14,15]. The observation of region-specific genotypes is reported in the literature [16,[18][19][20], and the presence of novel genotypes in Brazil and Argentina reinforces this cartographic feature of the genetic diversity based on MST typing of Q fever.…”

Section: Discussionmentioning

confidence: 75%

New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina

et al. 2019

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Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution. Despite the vast information about the circulating genotypes in Europe and North America, there is a lack of data regarding C. burnetii strains in South America. Here, we show the presence of novel multispacer sequence typing (MST) genotypes of C. burnetii in two clusters detected in Brazil and Argentina that seem to be distant in parenthood. Argentinian strains isolated from a tick belongs to a new phylogenetic branch of C. burnetii, and the Brazilians strains may be related to MST 20 and 61. Multilocus variable number tandem repeats analysis (MLVA) typing provided a deeper resolution that may be related to host clusters of bovines, caprine, ovine, and ticks. Our results corroborate with the reports of geotypes of C. burnetii. Thus, we highlight the need for more genotyping studies to understand the genetic diversity of C. burnetii in South America and to confirm the hypothesis of host-related genotypes. We also emphasize the importance of virulence studies for a better understanding of Q fever in the region, which may help in surveillance and disease prevention programs.

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Section: Discussionmentioning

confidence: 75%

New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina

et al. 2019

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“…Since 2003, six new whole genomes of C. burnetii have become available in GenBank (142)(143)(144). Sixteen incomplete genomes can also be found (27,47,(145)(146)(147)(148)(149)(150). In 2009, Beare et al performed a comparative genomic analysis of four strains and found a total of 125 pseudogenes, of which 65 were highly conserved, and 8 IS families (142).…”

Section: Comparative Genomics and Pangenomic Analysismentioning

confidence: 99%

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

et al. 2017

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Coxiella burnetii is the agent of Q fever, or "query fever," a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic. C. burnetii is classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture. C. burnetii infects a wide range of animals, from arthropods to humans. The genetic determinants of virulence are now better known, thanks to the achievement of determining the genome sequences of several strains of this species and comparative genomic analyses. Q fever can be found worldwide, but the epidemiological features of this disease vary according to the geographic area considered, including situations where it is endemic or hyperendemic, and the occurrence of large epidemic outbreaks. In recent years, a major breakthrough in the understanding of the natural history of human infection with C. burnetii was the breaking of the old dichotomy between "acute" and "chronic" Q fever. The clinical presentation of C. burnetii infection depends on both the virulence of the infecting C. burnetii strain and specific risks factors in the infected patient. Moreover, no persistent infection can exist without a focus of infection. This paradigm change should allow better diagnosis and management of primary infection and long-term complications in patients with C. burnetii infection.

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“…In Asia, limited studies on the C. burnetii genotype have been performed. The MST51 genotype was reported in Saudi Arabia in an endocarditis patient [ 29 ]. In Asia, as elsewhere in the world, more genotypic studies are needed to determine the geographical distribution of C. burnetii genotypes among humans and livestock infections.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

et al. 2022

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Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian domestic ruminants. Understanding the C. burnetii genotypic profiles is critical in determining and preventing Q fever outbreaks.

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Draft Genome Sequence of Coxiella burnetii Strain Cb196, an Agent of Endocarditis in Saudi Arabia

Cited by 5 publications

References 11 publications

New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina

New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

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