Draft Genome Sequence of Corynebacterium coyleae UMB8490, Isolated from the Female Urinary Tract

Khan, Aamir Ali; Miller-Ensminger, Taylor; Voukadinova, Adelina; Wolfe, Alan J.; Putonti, Catherine

doi:10.1128/mra.00393-20

Cited by 2 publications

References 12 publications

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Cataloging the Phylogenetic Diversity of Human Bladder Bacterial Isolates

Wolfe

Khemmani

et al. 2023

Preprint

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Although the human bladder is reported to harbor unique microbiota, our understanding of how these microbial communities interact with their human hosts is limited, mostly owing to the lack of isolates to test mechanistic hypotheses. Niche-specific bacterial collections and associated reference genome databases have been instrumental in expanding knowledge of the microbiota of other anatomical sites, e.g., the gut and oral cavity. To facilitate genomic, functional, and experimental analyses of the human bladder microbiota, here we present a bladder-specific bacterial reference collection comprised of 1134 genomes. These genomes were culled from bacterial isolates obtained by a metaculturomic method from bladder urine collected by transurethral catheterization. This bladder-specific bacterial reference collection includes 196 different species, including representatives of major aerobes and facultative anaerobes, as well as some anaerobes. It captures 72.2 % of the genera found when we reexamined previously published 16S rRNA gene sequencing of 392 adult female bladder urine samples. Comparative genomic analysis found that the taxonomies and functions of the bladder microbiota shared more similarities with the vaginal microbiota than the gut microbiota. Whole-genome phylogenetic and functional analyses of 186 bladderE. coliisolates and 387 gutE. coliisolates supports the hypothesis that phylogroup distribution and functions ofE. colistrains differ dramatically between these two very different niches. This bladder-specific bacterial reference collection is a unique resource that will enable hypothesis-driven bladder microbiota research and comparison to isolates from other anatomical sites.

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Cataloging the Phylogenetic Diversity of Human Bladder Bacterial Isolates

Wolfe

Khemmani

et al. 2023

Preprint

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Cataloging the phylogenetic diversity of human bladder bacterial isolates

Du,

Khemmani,

Halverson

et al. 2024

Genome Biol

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Background Although the human bladder is reported to harbor unique microbiota, our understanding of how these microbial communities interact with their human hosts is limited, mostly owing to the lack of isolates to test mechanistic hypotheses. Niche-specific bacterial collections and associated reference genome databases have been instrumental in expanding knowledge of the microbiota of other anatomical sites, such as the gut and oral cavity. Results To facilitate genomic, functional, and experimental analyses of the human bladder microbiota, we present a bladder-specific bacterial isolate reference collection comprising 1134 genomes, primarily from adult females. These genomes were culled from bacterial isolates obtained by a metaculturomic method from bladder urine collected by transurethral catheterization. This bladder-specific bacterial isolate reference collection includes 196 different species, including representatives of major aerobes and facultative anaerobes, as well as some anaerobes. It captures 72.2% of the genera found when re-examining previously published 16S rRNA gene sequencing of 392 adult female bladder urine samples. Comparative genomic analysis finds that the taxonomies and functions of the bladder microbiota share more similarities with the vaginal microbiota than the gut microbiota. Whole-genome phylogenetic and functional analyses of 186 bladder Escherichia coli isolates and 387 gut Escherichia coli isolates support the hypothesis that phylogroup distribution and functions of Escherichia coli strains differ dramatically between these two very different niches. Conclusions This bladder-specific bacterial isolate reference collection is a unique resource that will enable bladder microbiota research and comparison to isolates from other anatomical sites.

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Draft Genome Sequence of Corynebacterium coyleae UMB8490, Isolated from the Female Urinary Tract

Abstract: Here, we present the draft genome sequence for Corynebacterium coyleae UMB8490, isolated from the catheterized urine of a female with overactive bladder symptoms. The size of the genome is 2,483,223 bp assembled in 62 contigs with an observed GC content of 61.42%.

Cited by 2 publications

References 12 publications

Cataloging the Phylogenetic Diversity of Human Bladder Bacterial Isolates

Cataloging the Phylogenetic Diversity of Human Bladder Bacterial Isolates

Cataloging the phylogenetic diversity of human bladder bacterial isolates

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