Draft Genome Sequence of Chromobacterium vaccinii, a Potential Biocontrol Agent against Mosquito (Aedes aegypti) Larvae

Vöing, Kristin; Harrison, Alisha; Soby, Scott

doi:10.1128/genomea.00477-15

Cited by 29 publications

(23 citation statements)

References 5 publications

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“…This search and subsequent analysis using IMG-ABC tools took less than an hour, and has led to the identification of putative DAPG biosynthesis clusters in four betaproteobacteria: a bacterium identified as Pseudogulbenkiania ferrooxidans EGD-HP2 (19) and three members of Chromobacterium genus, Chromobacterium vaccinii MWU205 (20), C. vaccinii MWU328 (20) and Chromobacterium piscinae ND17 (21). The IMG tools for analysis of biogeography and habitat specificity of these isolates indicate that all of them were collected from freshwater environments across the globe (Northeastern India, Northeastern United States of America and Malaysia).…”

Section: New Features and Updatesmentioning

confidence: 99%

“…This finding is remarkable considering that so far DAPG production in bacteria has been described mostly in plant-associated bacteria and in a bacterial symbiont of the red-backed salamander (22). Interestingly, C. vaccinii strains, in which DAPG clusters were found, were studied because of their biocontrol activity against mosquito larvae (20). Since DAPG is known to elicit a lethal hyperactive immune response in Drosophila melanogaster larvae (23) and has known toxicity against other organisms (24), this finding raises the possibility that DAPG may be responsible for larvicidal activity of the C. vaccinii strains.…”

Section: New Features and Updatesmentioning

confidence: 99%

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IMG-ABC: new features for bacterial secondary metabolism analysis and targeted biosynthetic gene cluster discovery in thousands of microbial genomes

et al. 2016

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Secondary metabolites produced by microbes have diverse biological functions, which makes them a great potential source of biotechnologically relevant compounds with antimicrobial, anti-cancer and other activities. The proteins needed to synthesize these natural products are often encoded by clusters of co-located genes called biosynthetic gene clusters (BCs). In order to advance the exploration of microbial secondary metabolism, we developed the largest publically available database of experimentally verified and predicted BCs, the Integrated Microbial Genomes Atlas of Biosynthetic gene Clusters (IMG-ABC) (https://img.jgi.doe.gov/abc/). Here, we describe an update of IMG-ABC, which includes ClusterScout, a tool for targeted identification of custom biosynthetic gene clusters across 40 000 isolate microbial genomes, and a new search capability to query more than 700 000 BCs from isolate genomes for clusters with similar Pfam composition. Additional features enable fast exploration and analysis of BCs through two new interactive visualization features, a BC function heatmap and a BC similarity network graph. These new tools and features add to the value of IMG-ABC's vast body of BC data, facilitating their in-depth analysis and accelerating secondary metabolite discovery.

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Section: New Features and Updatesmentioning

confidence: 99%

Section: New Features and Updatesmentioning

confidence: 99%

IMG-ABC: new features for bacterial secondary metabolism analysis and targeted biosynthetic gene cluster discovery in thousands of microbial genomes

et al. 2016

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“…MWU3525 contained 17 probable chitinase genes, including nine probable chitinase A genes, and seven endochitinases. In contrast to C. vaccinii ( 8 , 9 ), none of the isolates contained putative hydrolase transmembrane family proteins.…”

Section: Genome Announcementmentioning

confidence: 69%

Draft Genome Sequences of Three Chromobacterium subtsugae Isolates from Wild and Cultivated Cranberry Bogs in Southeastern Massachusetts

2015

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“… Panama QARX00000000 This study Chromobacterium sp . W10 QARW00000000 This study C. aquaticum CC-SEYA-1 NZ_MQZY00000000.1 69 C. haemolyticum MDA0585 NZ_JONK00000000.1 70 C. piscinae ND17 NZ_JTGE00000000.1 71 C. pseudoviolaceum LMG 3953 NZ_MQZX00000000.1 72 C. subtsugae MWU2920 NZ_LCWP00000000.1 73 C. vaccinii IIBBL 21-1 NZ_CP017707.1 74 C. violaceum ATCC 12472 NC_005085.1 75 …”

Section: Methodsmentioning

confidence: 98%

Chromobacterium spp. mediate their anti-Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin

Saraiva

Huitt-Roehl

Tripathi

et al. 2018

Sci Rep

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The Chromobacterium sp. Panama bacterium has in vivo and in vitro anti-Plasmodium properties. To assess the nature of the Chromobacterium-produced anti-Plasmodium factors, chemical partition was conducted by bioassay-guided fractionation where different fractions were assayed for activity against asexual stages of P. falciparum. The isolated compounds were further partitioned by reversed-phase FPLC followed by size-exclusion chromatography; high resolution UPLC and ESI/MS data were then collected and revealed that the most active fraction contained a cyclic depsipeptide, which was identified as romidepsin. A pure sample of this FDA-approved HDAC inhibitor allowed us to independently verify this finding, and establish that romidepsin also has potent effect against mosquito stages of the parasite’s life cycle. Genomic comparisons between C. sp. Panama and multiple species within the Chromobacterium genus further demonstrated a correlation between presence of the gene cluster responsible for romidepsin production and effective antiplasmodial activity. A romidepsin-null Chromobacterium spp. mutant loses its anti-Plasmodium properties by losing the ability to inhibit P. falciparum HDAC activity, and romidepsin is active against resistant parasites to commonly deployed antimalarials. This independent mode of action substantiates exploring a chromobacteria-based approach for malaria transmission-blocking.

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Draft Genome Sequence of Chromobacterium vaccinii, a Potential Biocontrol Agent against Mosquito (Aedes aegypti) Larvae

Cited by 29 publications

References 5 publications

IMG-ABC: new features for bacterial secondary metabolism analysis and targeted biosynthetic gene cluster discovery in thousands of microbial genomes

IMG-ABC: new features for bacterial secondary metabolism analysis and targeted biosynthetic gene cluster discovery in thousands of microbial genomes

Draft Genome Sequences of Three Chromobacterium subtsugae Isolates from Wild and Cultivated Cranberry Bogs in Southeastern Massachusetts

Chromobacterium spp. mediate their anti-Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin

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