Draft Genome Sequence of Catellicoccus marimammalium, a Novel Species Commonly Found in Gull Feces

Weigand, Michael R.; Ryu, Hodon; Bozcek, Laura; Konstantinidis, Konstantinos T.; Domingo, Jorge W. Santo

doi:10.1128/genomea.00019-12

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…Bacteria from the genus Catellicoccus have also been found in the gastrointestinal tract of zebra finches ( Taeniopygia guttata ) 41 ; barn swallows ( Hirundo rustica ) 42 ; red knots ( Calidris canutus ) and ruddy turnstones ( Arenaria interpres ) 43 ; black-tailed godwits ( Limosa limosa ), black-winged stilts ( Himantopus himantopus ) and common redshanks ( Tringa totanus ) 44 ; black-headed gulls ( Chroicocephalus ridibundus ) 45 and waterfowl 46 . The genome of Catellicoccus marimammalium revealed that this bacterium encodes various functions such as nutrient transport and bile acid hydrolysis suggesting a symbiotic lifestyle of this species 46 . Possible beneficial effects for the host such as immune modulation and gut maturation have also been proposed for bacteria of this genus inhabiting the avian gut 41 .…”

Section: Discussionmentioning

confidence: 99%

Gut microbiome is affected by inter-sexual and inter-seasonal variation in diet for thick-billed murres (Uria lomvia)

Góngora

Elliott

Whyte

2021

Sci Rep

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The role of the gut microbiome is increasingly being recognized by health scientists and veterinarians, yet its role in wild animals remains understudied. Variations in the gut microbiome could be the result of differential diets among individuals, such as variation between sexes, across seasons, or across reproductive stages. We evaluated the hypothesis that diet alters the avian gut microbiome using stable isotope analysis (SIA) and 16S rRNA gene sequencing. We present the first description of the thick-billed murre (Uria lomvia) fecal microbiome. The murre microbiome was dominated by bacteria from the genus Catellicoccus, ubiquitous in the guts of many seabirds. Microbiome variation was explained by murre diet in terms of proportion of littoral carbon, trophic position, and sulfur isotopes, especially for the classes Actinobacteria, Bacilli, Bacteroidia, Clostridia, Alphaproteobacteria, and Gammaproteobacteria. We also observed differences in the abundance of bacterial genera such as Catellicoccus and Cetobacterium between sexes and reproductive stages. These results are in accordance with behavioural observations of changes in diet between sexes and across the reproductive season. We concluded that the observed variation in the gut microbiome may be caused by individual prey specialization and may also be reinforced by sexual and reproductive stage differences in diet.

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Section: Discussionmentioning

confidence: 99%

Gut microbiome is affected by inter-sexual and inter-seasonal variation in diet for thick-billed murres (Uria lomvia)

Góngora

Elliott

Whyte

2021

Sci Rep

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“…Enterococcus spp. typically have four to six rrnA operons, whereas C. marimammalium have only one, so these are minimum estimates of the relative fold enrichment (41,42). Comparing the quantity of enterococci cultured from our four gull fecal samples, which averaged 2.3 ϫ 10 4 CFU g Ϫ1 , we can estimate that there would be over 3 orders of magnitude greater density (ϳ1.1 ϫ 10 8 cells g Ϫ1 ) of C. marimammalium also present.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Gull Fecal Microbial Community Reveals the Dominance of Catellicoccus marimammalium in Relation to Culturable Enterococci

Koskey

Fisher

Traudt

et al. 2014

Appl Environ Microbiol

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Gulls are prevalent in beach environments and can be a major source of fecal contamination. Gulls have been shown to harbor a high abundance of fecal indicator bacteria (FIB), such as Escherichia coli and enterococci, which can be readily detected as part of routine beach monitoring. Despite the ubiquitous presence of gull fecal material in beach environments, the associated microbial community is relatively poorly characterized. We generated comprehensive microbial community profiles of gull fecal samples using Roche 454 and Illumina MiSeq platforms to investigate the composition and variability of the gull fecal microbial community and to measure the proportion of FIB. Enterococcaceae and Enterobacteriaceae were the two most abundant families in our gull samples. Sequence comparisons between short-read data and nearly full-length 16S rRNA gene clones generated from the same samples revealed Catellicoccus marimammalium as the most numerous taxon among all samples. The identification of bacteria from gull fecal pellets cultured on membrane-Enterococcus indoxyl-␤-D-glucoside (mEI) plates showed that the dominant sequences recovered in our sequence libraries did not represent organisms culturable on mEI. Based on 16S rRNA gene sequencing of gull fecal isolates cultured on mEI plates, 98.8% were identified as Enterococcus spp., 1.2% were identified as Streptococcus spp., and none were identified as C. marimammalium. Illumina deep sequencing indicated that gull fecal samples harbor significantly higher proportions of C. marimammalium 16S rRNA gene sequences (>50-fold) relative to typical mEI culturable Enterococcus spp. C. marimammalium therefore can be confidently utilized as a genetic marker to identify gull fecal pollution in the beach environment.

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“…interact with the host at some level. Interestingly, genome analysis of C. marimammalium has suggested that this bacterium is transitioning from a commensal into an obligate symbiont (40). Genome sequences of additional isolates are needed to determine if other Catellicoccus-like species have similar reduced metabolic pathways that are compatible with a symbiotic lifestyle.…”

Section: Resultsmentioning

confidence: 99%

Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay

Ryu

Grond

Verheijen

et al. 2014

Appl Environ Microbiol

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dUsing 16S rRNA gene sequencing analysis, we examined the bacterial diversity and the presence of opportunistic bacterial pathogens (i.e., Campylobacter and Helicobacter) in red knot (Calidris canutus; n ‫؍‬ 40), ruddy turnstone (Arenaria interpres; n ‫؍‬ 35), and semipalmated sandpiper (Calidris pusilla; n ‫؍‬ 22) fecal samples collected during a migratory stopover in Delaware Bay. Additionally, we studied the occurrence of Campylobacter spp., enterococci, and waterfowl fecal source markers using quantitative PCR (qPCR) assays. Of 3,889 16S rRNA clone sequences analyzed, the bacterial community was mostly composed of Bacilli (63.5%), Fusobacteria (12.7%), Epsilonproteobacteria (6.5%), and Clostridia (5.8%). When epsilonproteobacterium-specific 23S rRNA gene clone libraries (i.e., 1,414 sequences) were analyzed, the sequences were identified as Campylobacter (82.3%) or Helicobacter (17.7%) spp. Specifically, 38.4%, 10.1%, and 26.0% of clone sequences were identified as C. lari (>99% sequence identity) in ruddy turnstone, red knot, and semipalmated sandpiper clone libraries, respectively. Other pathogenic species of Campylobacter, such as C. jejuni and C. coli, were not detected in excreta of any of the three bird species. Most Helicobacter-like sequences identified were closely related to H. pametensis (>99% sequence identity) and H. anseris (92% sequence identity). qPCR results showed that the occurrence and abundance of Campylobacter spp. was relatively high compared to those of fecal indicator bacteria, such as Enterococcus spp., E. faecalis, and Catellicoccus marimammalium. Overall, the results provide insights into the complexity of the shorebird gut microbial community and suggest that these migratory birds are important reservoirs of pathogenic Campylobacter species.

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Draft Genome Sequence of Catellicoccus marimammalium, a Novel Species Commonly Found in Gull Feces

Abstract: Catellicoccus marimammalium is a relatively uncharacterized Gram-positive facultative anaerobe with potential utility as an indicator of waterfowl fecal contamination. Here, we report an annotated draft genome sequence that suggests that this organism may be a symbiotic gut microbe.

Cited by 9 publications

References 11 publications

Gut microbiome is affected by inter-sexual and inter-seasonal variation in diet for thick-billed murres (Uria lomvia)

Gut microbiome is affected by inter-sexual and inter-seasonal variation in diet for thick-billed murres (Uria lomvia)

Analysis of the Gull Fecal Microbial Community Reveals the Dominance of Catellicoccus marimammalium in Relation to Culturable Enterococci

Intestinal Microbiota and Species Diversity of Campylobacter and Helicobacter spp. in Migrating Shorebirds in Delaware Bay

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