Draft Genome Sequence of Bacillus thuringiensis var.
            <i>thuringiensis</i>
            Strain T01-328, a Brazilian Isolate That Produces a Soluble Pesticide Protein, Cry1Ia

Varani, Alessandro M.; Lemos, Manoel Victor Franco; Fernandes, Camila Cesário; Lemos, Eliana Gertrudes Macedo; Alves, Eliane C. C.; Desidério, Janete Apparecida

doi:10.1128/genomea.00817-13

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…And the following PCR detection experiment using primers specific for each of the genes confirmed our speculation ( Figure 5 ). On the other hand, the Cry1Ia protein was a secreted delta-endotoxin produced by several Bt strains during vegetative growth and could not form a classic crystal like other Cry proteins ( Dammak et al, 2011 ; Varani et al, 2013 ). Thus, it was not surprising that Cry1Ia and Vip3A proteins were not detected during the spore-release period of X022.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Genomics and Proteomics in the New Isolated Bacillus thuringiensis X022 Revealed the Metabolic Regulation Mechanism of Carbon Flux Following Cu2+ Treatment

et al. 2016

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Bacillus thuringiensis (Bt) X022 is a novel strain isolated from soil in China, and showed strong insecticidal activity against several Lepidopteran pests. In this work, we performed whole genome sequencing of this Bt strain using the next-generation sequencing technology, and further conducted a comparative analysis with the proteomics data of the specific spore-release period based on LC-MS/MS approach. The Bt X022 genome consisted of one circular chromosomal DNA and seven plasmids, which were further functionally annotated using the RAST server. Comparative analysis of insecticidal substances showed that X022 contained genes coding for three Cry proteins (Cry1Ac, Cry1Ia and Cry2Ab) and a vegetative insecticidal protein (Vip3A). However, three insecticidal crystal proteins (ICPs) (Cry1Ca, Cry1Ac and Cry1Da) were detected by proteomics in the spore-release period. Moreover, a putative biosynthetic gene cluster and the metabolic pathway for poly-β-hydroxybutyrate in Bt X022 were deduced based on the comparative analysis of genomic and proteomic data, which revealed the metabolic regulation mechanism of carbon flux correlated with increased production of ICPs caused by Cu2+. Hence, these results provided a deeper understanding of the genetic background and protein expression profile of Bt X022. This study established a foundation for directed genetic modification and further application of this new isolated Bt strain.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Genomics and Proteomics in the New Isolated Bacillus thuringiensis X022 Revealed the Metabolic Regulation Mechanism of Carbon Flux Following Cu2+ Treatment

et al. 2016

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“…Masson et al [77] observed that the encoded Cry1I protein did not accumulate in crystals in Bt strain HD-133 [78,79]. The Cry1I protein therefore could not be found in the bacterial cell after the T5 sporulation stage [80,81].…”

Section: No Known Crystallization Factorsmentioning

confidence: 99%

In Vivo Crystallization of Three-Domain Cry Toxins

Adalat

Saleem

Crickmore

et al. 2017

Toxins

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Bacillus thuringiensis (Bt) is the most successful, environmentally-friendly, and intensively studied microbial insecticide. The major characteristic of Bt is the production of proteinaceous crystals containing toxins with specific activity against many pests including dipteran, lepidopteran, and coleopteran insects, as well as nematodes, protozoa, flukes, and mites. These crystals allow large quantities of the protein toxins to remain stable in the environment until ingested by a susceptible host. It has been previously established that 135 kDa Cry proteins have a crystallization domain at their C-terminal end. In the absence of this domain, Cry proteins often need helper proteins or other factors for crystallization. In this review, we classify the Cry proteins based on their requirements for crystallization.

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