Draft Genome Sequence of Bacillus stratosphericus LAMA 585, Isolated from the Atlantic Deep Sea

Lima, André Oliveira de Souza; Cabral, Alencar; Andreote, Fernando Dini; Cavalett, Angélica; Pessatti, Marcos Luiz; Dini‐Andreote, Francisco; Silva, Marcus Adonai Castro da

doi:10.1128/genomea.00204-13

Cited by 10 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, B. altitudinis and B. stratosphericus were first isolated in the stratosphere at 41 km above the earth’s surface and reported by (81). In contrast, Lima et al, 2013 (82) reported another B. stratosphericus strain LAMA 585 isolated in the Atlantic deep sea at 5,500 m depth. All these species of Bacillus are widely distributed on the earth, but instead of B. safensis, strains of B. altitudinis and B. stratosphericus were found most frequently in marine environments (80).…”

Section: Discussionmentioning

confidence: 94%

Uncovering the diversity of heterotrophic culturable bacteria within the gut of bay scallopArgopecten purpuratus(Lamarck, 1819) from two geographically distant natural banks

Serrano,

Tarazona-Janampa,

Olaechea

et al. 2023

Preprint

View full text Add to dashboard Cite

We study the bacterial diversity within the gut of the peruvian bay scallopArgopecten purpuratus(Lamarck, 1819) (Mollusca: Pectinidae) collected from two shellfish growing areas in Independence Bay (IB) and Sechura Bay (SB) in Peru. This work aimed to identify isolates of culturable gut-associated microbiota ofA. purpuratusthrough repetitive sequence-based PCR (rep-PCR) and 16S rRNA gene sequences comparison. A total of 20 healthy adult scallops were collected from each location twice, in spring and autumn, 2015 and 2016, respectively.Fecal pellets from the distal intestinal tract of each individual were extracted, and after pooling, samples were cultivated on general and selective media following standard culture conditions. In order to prevent duplicate sequencing of the ribosomal 16S rRNA gene sequences of the isolates, a pre-screened step by (rep-PCR) amplification utilizing BOXA1R primer was performed, then isolates showing different banding profiles were selected for further sequencing.Taxonomic distribution based on the 16S rRNA gene comparison of selected strains placed isolates from IB and SB into 29 and 27 different genera, representing 50 and 42 species, respectively. The major proportions of bacterial species found in IB corresponded to the division Firmicutes (46%), whereas the Proteobacteria and Firmicutes were almost equally distributed in (SB) with 38 and 43% respectively. Remarkable, (IB) showed a high number (28%) and diversity of species within the division Actinobacteria compared to (SB); while the speciesSalinimicrobiumsp. of the division Flavobacteria was isolated only in (SB).Common species in both areas are mainly related to the genusBacillus,while strains of speciesVibrio alginolyticusandV. parahaemoliticuswere common representatives of the Proteobacteria. The intestinal tract ofA. purpuratusmay represent an uninvestigated niche for analyzing the structure of new complex microbial communities. This study showed that the diversity of culturable microbiota within the gut of two populations ofA. purpuratusis significantly different at each location.

show abstract

Section: Discussionmentioning

confidence: 94%

Uncovering the diversity of heterotrophic culturable bacteria within the gut of bay scallopArgopecten purpuratus(Lamarck, 1819) from two geographically distant natural banks

Serrano,

Tarazona-Janampa,

Olaechea

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Bacillus stratosphericus is a Gram-positive endospore forming bacterium that belongs to phylum Firmicutes . It occupies quite distinct habitats, originally isolated from stratospheric air samples collected above 24 km of altitude and recently from the Mid-Atlantic-Ridge seafloor (5,500 m depth ( 1 , 2 ). In this study, Bacillus stratosphericus strain 5Co was isolated from medicinally important fruticose lichen Usnea florida that grows luxuriously in the central Florida region of the United States.…”

Section: Genome Announcementmentioning

confidence: 99%