Draft Genome Sequence of Bacillus paralicheniformis F47, Isolated from an Algerian Salty Lake

Daas, Mohamed Seghir; Rosana, Albert Remus R.; Acedo, Jeella Z.; Douzane, Malika; Natèche, Farida; Kebbouche-Gana, Salima; Vederas, John C.

doi:10.1128/genomea.00190-18

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…In a previous study, the isolation of the bacteria B. paralicheniformis bac48 and bac84 from the Red Sea environment was reported by Othoum et al (2018) [26]. Dass et al (2018) isolated B. paralicheniformis F47 from a salty lake in Algeria [27]. Here, our MLST analysis showed that our three strains were closely related to B. paralicheniformis species.…”

Section: Isolation Of Cellulase-producing Microorganismssupporting

confidence: 73%

Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea

et al. 2021

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Background Cellulolytic microorganisms are considered a key player in the degradation of plant biomass in various environments. These microorganisms can be isolated from various environments, such as soils, the insect gut, the mammalian rumen and oceans. The Red Sea exhibits a unique environment in terms of presenting a high seawater temperature, high salinity, low nutrient levels and high biodiversity. However, there is little information regarding cellulase genes in the Red Sea environment. This study aimed to examine whether the Red Sea can be a resource for the bioprospecting of microbial cellulases by isolating cellulase-producing microorganisms from the Red Sea environment and characterizing cellulase genes. Results Three bacterial strains were successfully isolated from the plankton fraction and the surface of seagrass. The isolated strains were identified as Bacillus paralicheniformis and showed strong cellulase activity. These results suggested that these three isolates secreted active cellulases. By whole genome sequencing, we found 10 cellulase genes from the three isolates. We compared the expression of these cellulase genes under cellulase-inducing and non-inducing conditions and found that most of the cellulase genes were generally upregulated during cellulolysis in the isolates. Our operon structure analysis also showed that cellulase genes form operons with genes involved in various kinds of cellular reactions, such as protein metabolism, which suggests the existence of crosstalk between cellulolysis and other metabolic pathways in the bacterial isolates. These results suggest that multiple cellulases are playing important roles in cellulolysis. Conclusions Our study reports the isolation and characterization of cellulase-producing bacteria from the Red Sea. Our whole-genome sequencing classified our three isolates as Bacillus paralicheniformis, and we revealed the presence of ten cellulase orthologues in each of three isolates’ genomes. Our comparative expression analysis also identified that most of the cellulase genes were upregulated under the inducing conditions in general. Although cellulases have been roughly classified into three enzyme groups of beta-glucosidase, endo-β-1,4-glucanase and exoglucanase, these findings suggest the importance to consider microbial cellulolysis as a more complex reaction with various kinds of cellulase enzymes.

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Section: Isolation Of Cellulase-producing Microorganismssupporting

confidence: 73%

Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea

et al. 2021

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“…The B. paralicheniformis urease gene cluster may not be involved in human disease, and rather in nitrogen utilization, considering its habitat. The majorities of Bacillus spp., including B. paralicheniformis, have been isolated from soil and the rhizosphere [19,20]. However, we cannot speculate on the results were this species to be used for human or animal food production, because no report of its safety aspects has yet been published.…”

Section: Discussionmentioning

confidence: 99%

Urease Characteristics and Phylogenetic Status of Bacillus paralicheniformis

Jeong¹,

Lee²,

Lee³

et al. 2018

Journal of Microbiology and Biotechnology

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In 2015, Bacillus paralicheniformis was separated from B. licheniformis on the basis of phylogenomic and phylogenetic studies, and urease activity was reported as a phenotypic property that differentiates between the two species. Subsequently, we have found that the urease activity of B. paralicheniformis is strain-specific, and does not reliably discriminate between species, as strains having the same urease gene cluster were identified in B. licheniformis and B. sonorensis, the closest relatives of B. paralicheniformis. We developed a multilocus sequence typing scheme using eight housekeeping genes, adk, ccpA, glpF, gmk, ilvD, pur, spo0A, and tpi to clearly identify B. paralicheniformis from closely related Bacillus species and to find a molecular marker for the rapid identification of B. paralicheniformis. The scheme differentiated 33 B. paralicheniformis strains from 90 strains formerly identified as B. licheniformis. Among the eight housekeeping genes, spo0A possesses appropriate polymorphic sites for the design of a B. paralichenofomis-specific PCR primer set. The primer set designed in this study perfectly separated B. paralicheniformis from B. licheniformis and B. sonorensis.

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“… Bacillus paralicheniformis , a Gram-positive bacillus cultured in medium with up to 10% NaCl, was described as a new species in 2015 ( 1 ). It controls plant pathogens and produces bioactive secondary metabolites ( 2 ). We present the draft genome sequence of strain TXO7B-1SG6, isolated from hypersaline sediment from Lake Texcoco, Mexico (19°28′42″N, 98°58′26'″W), in June 2019 and identified as Bacillus paralicheniformis .…”

Section: Announcementmentioning

confidence: 99%

Bacillus paralicheniformis isolated from a hypersaline sediment of Lake Texcoco, draft genome sequence of strain TXO7B-1SG6

Can-Ubando

Sánchez-Reyes

Manzanares-Leal

et al. 2022

Microbiol Resour Announc

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Draft Genome Sequence of Bacillus paralicheniformis F47, Isolated from an Algerian Salty Lake

Cited by 7 publications

References 18 publications

Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea

Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea

Urease Characteristics and Phylogenetic Status of Bacillus paralicheniformis

Bacillus paralicheniformis isolated from a hypersaline sediment of Lake Texcoco, draft genome sequence of strain TXO7B-1SG6

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