Draft Genome Sequence of Aryl Hydrocarbon Receptor Activator Strains Lactobacillus reuteri R2lc and 2010

Özçam, Mustafa; Pijkeren, Jan-Peter van

doi:10.1128/mra.00067-19

Cited by 3 publications

(2 citation statements)

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“…Limosilactobacillus reuteri, until recently known as Lactobacillus reuteri (Zheng et al, 2020) is a gut symbiont species that inhabits the gastrointestinal tract of various vertebrates, including rodents, birds and primates (Duar et al, 2017). This, combined with the available genome editing tools (Oh and Van Pijkeren, 2014; Zhang et al, 2018), make L. reuteri an ideal model organism to study microbe-microbe, microbe-phage and microbehost interactions (Lin et al, 2018;Oh et al, 2019;Özçam et al, 2019;Walter et al, 2011). Previously, we identified two genetically distinct PKS clusters (pks and fun) in L. reuteri R2lc that activated the Aryl Hydrocarbon Receptor (Özçam and van Pijkeren, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…This, combined with the available genome editing tools (Oh and Van Pijkeren, 2014; Van Pijkeren et al, 2012; Zhang et al, 2018), make L. reuteri an ideal model organism to study microbe-microbe, microbe-phage and microbe-host interactions (Lin et al, 2018; Oh et al, 2019; Özçam et al, 2019; Walter et al, 2011). Previously, we identified two genetically distinct PKS clusters ( pks and fun ) in L. reuteri R2lc that activated the Aryl Hydrocarbon Receptor (Özçam and van Pijkeren, 2019).…”

Section: Introductionmentioning

confidence: 99%

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A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell wall acetylation

Özçam

Tocmo

et al. 2021

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The mammalian microbiome encodes numerous secondary metabolite biosynthetic gene clusters, yet their role in microbe-microbe interactions is unclear. Here, we characterized two polyketide synthase gene clusters (fun and pks) in the gut symbiont Limosilactobacillus reuteri. The pks, but not the fun cluster, encodes antimicrobial activity. Forty-one out of 51 L. reuteri strains tested are sensitive to Pks products, which was independent of strains host origin. The sensitivity to Pks was also established in intraspecies competition experiments in gnotobiotic mice. Comparative genome analyses between Pks-resistant and sensitive strains identified an acyltransferase gene (act) that is unique to Pks-resistant strains. Subsequent peptidoglycan analysis of the wild-type and the act mutant strains showed that Act acetylates peptidoglycan. The pks mutants lost their competitive advantage and act mutants lost their Pks resistance in vivo. Thus, our findings provide mechanistic insights into how closely related gut symbionts can compete and co-exist in the gastrointestinal tract.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell wall acetylation

Özçam

Tocmo

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell-wall acetylation

Özçam

Tocmo

et al. 2022

Cell Host & Microbe

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Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

et al. 2021

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Background Intestinal Peyer’s patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation. Results The B cells of PPs were separated by size to circumvent activation-dependent cell identification biases due to dynamic expression of markers, which resulted in two phenotypically, transcriptionally, and spatially distinct subsets: small IgD+/GL7−/S1PR1+/Bcl6, CCR6-expressing pre-germinal center (GC)-like B cells with innate-like functions located subepithelially, and large GL7+/S1PR1−/Ki67+/Bcl6, CD69-expressing B cells with strong metabolic activity found in the GC. Peroral L. reuteri administration expanded both B cell subsets and enhanced the innate-like properties of pre-GC-like B cells while retaining them in the sub-epithelial compartment by increased sphingosine-1-phosphate/S1PR1 signaling. Furthermore, L. reuteri promoted GC-like B cell differentiation, which involved expansion of the GC area and autocrine TGFβ-1 activation. Consequently, PD-1-T follicular helper cell-dependent IgA induction and production was increased by L. reuteri, which shifted the intestinal microbiome and protected against dextran-sulfate-sodium induced colitis and dysbiosis. Conclusions The Peyer’s patches sense, enhance and transmit probiotic signals by increasing the numbers and effector functions of distinct B cell subsets, resulting in increased IgA production, altered intestinal microbiota, and protection against inflammation.

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Draft Genome Sequence of Aryl Hydrocarbon Receptor Activator Strains Lactobacillus reuteri R2lc and 2010

Abstract: Lactobacillus reuteri R2lc and 2010 are pigmented rat intestinal isolates. L. reuteri R2lc has been studied in different animal disease models, including colitis and acute liver injury.

Cited by 3 publications

References 15 publications

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell wall acetylation

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell wall acetylation

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell-wall acetylation

Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

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