Draft genome sequence of antimicrobial producing Paenibacillus alvei strain MP1 reveals putative novel antimicrobials

Pajor, Magdalena; Sogin, Jonathan; Worobo, Randy W.; Szweda, Piotr

doi:10.1186/s13104-020-05124-z

Cited by 7 publications

(5 citation statements)

References 31 publications

(33 reference statements)

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“…One of these strains, namely Paenibacillus alvei MP1, was found to be an efficient producer of proteinaceous agent that exhibited promising activity against a broad spectrum of pathogenic bacteria [40]. Moreover, several genes responsible for antimicrobial activity have been identified in the genome of P. alvei MP1 [41]. Some other research groups have also reported the isolation of antimicrobials producing bacteria from honey.…”

Section: Discussionmentioning

confidence: 99%

Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials

et al. 2021

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The principal objective of the study was the isolation and identification of bacteria that are present in mature bee bread (BB) and dried (ready for selling and consumption) bee pollen (BP). Obtained isolates were screened for their potential to inhibit select human pathogenic bacteria and their ability to produce enzymes of particular industrial importance. Four and five samples of BP and BB, respectively, were used for the study. In total, 81 strains of bacteria were isolated, and 34 (42%) of them exhibited antagonistic interactions with at least one reference strain of pathogenic bacteria, namely Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis 12228, Pseudomonas aeruginosa ATCC 27857, and Escherichia coli ATCC 25922. The sequencing of the 16S rRNA gene revealed that all strains producing antimicrobials belong to the genus Bacillus spp., and among them, five species were identified: B. pumilus (n = 17), B. altitudinis (n = 9), B. licheniformis (n = 4), B. subtilis (n = 2), and B. safensis (n = 1). Furthermore, 69, 54, 39, and 29 of the strains exhibited lipolytic, proteolytic, cellulolytic, and esterolytic activity, respectively. Alpha amylase and beta galactosidase activity were rarely observed, and none of the strains produced laccase. The outcomes of the study revealed that BP and BB can be considered potential sources of bacteria producing antimicrobial agents and/or enzymes of particular industrial importance. Of course, additional research is required to verify this hypothesis, but the results of preliminary studies are promising.

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Section: Discussionmentioning

confidence: 99%

Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials

et al. 2021

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“…Characterizing the secondary by-products of the P. alvei strain recovered in the current study would help determine potential mechanism(s) of E. coli O157:H7 reduction due to these compounds. For example, Pajor et al (2020a) observed antimicrobial activity of P. alvei MP1 against E. coli O157:H7, and the heat-stable proteinaceous compounds identified in their study continue to be investigated (Pajor et al 2020b). Minimal reduction in E. coli O157:H7 was observed in filtered soil rinse subjected to P. polymyxa ATCC 21830, which has previously been documented as antagonistic through antibiotic production mechanisms (Naghmouchi et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil

Baker

Schneider

2021

J of Applied Microbiology

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Aims: This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined. Methods and Results: Escherichia coli O157:H7 counts (log CFU per ml or g À1 ) were determined by spread plating: (i) artificial soil amended with soil rinse (filter-sterilized and unfiltered) at 30°C; (ii) unfiltered soil rinse (50 ml) treated with cycloheximide (200 lg ml À1 ), vancomycin (40 lg ml À1 ), heat (80°C, 15 min) and no treatment (control) for 7 days at 30°C and (iii) filtered soil rinse with selected soil bacterial isolates over 7 days. There was a significant difference (P = 0Á027) in E. coli O157:H7 counts after 35 days between artificial soils amended with filtered (4Á45 AE 0Á29) and non-filtered (1Á83 AE 0Á33) soil rinse. There were significant differences (P < 0Á05) in E. coli O157:H7 counts after 3 days of incubation between soil rinse treatments (heat (7Á04 AE 0Á03), cycloheximide (6Á94 AE 0Á05), vancomycin (4Á26 AE 0Á98) and control (5Á00 AE 0Á93)). Lastly, a significant difference (P < 0Á05) in E. coli O157:H7 counts was observed after 3 days of incubation at 30°C in filtered soil rinse when incubated with Paenibacillus alvei versus other soil bacterial isolates evaluated. Conclusions: Soil microbiota isolated from Florida sandy soil influenced E. coli O157:H7 survival. Specifically, P. alvei reduced E. coli O157:H7 by over 3 log CFU per ml after 3 days of incubation at 30°C in filtered soil rinse. Significance and Impact of the Study: This research identified soil bacterial isolates that may reduce E. coli O157:H7 in the soil environment and be used in future biocontrol applications.

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“…For example, other Paenibacillus spp. that produce antimicrobials [47][48][49] or degrade complex carbohydrates [50,51] have been isolated from environmental or plant sources.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (Canis lupus)

McCabe,

Bryant,

Klews

et al. 2023

Applied Microbiology

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The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria. A bacterium identified as a novel Paenibacillus sp. strain via small ribosomal RNA (16S) gene sequencing was isolated from the GI tract of a gray wolf (Canis lupus). The bacterium was typed as Gram-variable, both catalase/oxidase-positive and positive via starch hydrolysis and lipase assays. The bacterium inhibited the growth of Staphylococcus aureus, Escherichia coli and Micrococcus luteus. The draft whole genome sequence (WGS) assembly was 7,034,206 bp in length, encoding 6543 genes, and is similar in size and coding capacity to other closely related Paenibacillus spp. The isolate’s genome encodes several germination and sporulation gene products along with antimicrobials such as a bacteriocin system and chitinase. Enzyme genes such as alpha amylase, cellulase, lipases and pectin lyase are also present in the genome. An incomplete lysogenic bacteriophage genome was also present in the isolate’s genome. Phenotypic characteristics combined with a WGS genotype analysis indicate that this bacterium, designated Paenibacillus sp. ClWae2A, could be a potential candidate probiotic for domestic dogs.

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Draft genome sequence of antimicrobial producing Paenibacillus alvei strain MP1 reveals putative novel antimicrobials

Cited by 7 publications

References 31 publications

Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials

Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials

Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil

Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (Canis lupus)

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