Draft Genome Sequence of an Escherichia coli Sequence Type 155 Strain Isolated from Sewage in Kerala, India

Salim, Amrita; Babu, Pradeesh; Mohan, Keerthi; Moorthy, Manju; Raj, Devika; Thirumeni, Swathy Kallampillil; Suresh, Sagarbabu; Madhavan, Ajith; Nair, Bipin; Chattopadhyay, Sujay; Pal, Sanjay

doi:10.1128/mra.01707-18

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…This finding demonstrates that the co-colonization of antimicrobial-resistant E. coli from a shared source is also possible. There is documentary evidence that E. coli ST-155 represents an important zoonotic strain responsible for the transmission of ESBL-EC to humans [33,34]. It is also important to note that ST-155 has been detected in E. coli isolates from water samples [34] and this is in agreement with findings from our study where this ST was detected in isolates from environmental samples.…”

Section: Discussionsupporting

confidence: 91%

Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria

et al. 2020

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Background Globally, chicken is known to be a reservoir for the spread of antimicrobial resistance genes to humans. In Nigeria, antimicrobial drugs are readily accessible for use in poultry production, either for preventive or therapeutic purposes. Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) are transmissible to humans because of their zoonotic potentials. People working very closely with chickens either on farms or markets are at greater risk. The aim of this study was to investigate the prevalence and zoonotic transmission of ESBL-EC among poultry-workers, chickens, and poultry environments in Abuja, Nigeria. Methods We conducted a cross-sectional study among workers, chickens and poultry environment in selected farms/chicken markets in Abuja. Stool, faecal, and environmental samples were collected from apparently healthy workers, chickens, and farm/market environments from December 2018 to April 2019. Data were collected electronically using an open data kit (ODK) installed on a Smartphone. Antimicrobial resistance was determined using broth micro-dilution methods against a panel of 14 antimicrobial agents. We carried out the phenotypic and genotypic characterization of the isolates. Data were analyzed by computing frequencies, proportions and spearman’s correlation (ρ). Results Of 429 samples, 26.8% ( n = 115) were positive for Escherichia coli (E. coli) . Of the 115 E. coli isolates, 32.2% ( n = 37) were confirmed ESBL producers by phenotypic characterization. Prevalence of ESBL-EC was highest among both poultry-workers (37.8%; n = 14) and chickens (37.8%; n = 14) followed by the environment (24.3%; n = 9). Both human and chicken isolates showed similar patterns of multidrug resistance to tested antimicrobials with a positive correlation (ρ = 0.91). Among ESBL producers, we observed the dissemination of bla CTX-M (10.8%; n = 4) genes. The coexistence of bla CTX-M-15 and bla TEM-1 genes was observed in 8.1% ( n = 3) of the isolates, out of which (66.7%; n = 2) were chicken isolates from the farm, while a single human isolate was from the chicken market. Conclusions ESBL-EC isolates were prevalent amongst apparently healthy individuals, chickens and the poultry farm/market environment in Abuja. It is important to educate healthcare workers that people in proximity with poultry are a high-risk group for faecal carriage of ESBL-EC, hence pose a higher risk to the general population for the spread of antimicrobial resistance.

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Section: Discussionsupporting

confidence: 91%

Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria

et al. 2020

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“…In the present study, ST155, ST162, ST359, and ST354 were predominantly detected in E. coli isolates having mutations in QRDR and/or PMQR genes. These clones were previously reported to be associated with human and animal infections [ 46 , 47 , 48 ]. In addition, E. coli ST38 and ST155 are considered high-risk clones that disperse antibiotic resistance on a global scale.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines

et al. 2021

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Antimicrobial resistance to quinolones, which constitutes a threat to public health, has been increasing worldwide. In this study, we investigated the prevalence of quinolone-resistant determinants in Escherichia coli not susceptible to quinolones and isolated from food-producing animals and food derived from them, in the Philippines. A total of 791 E. coli strains were isolated in 56.4% of 601 beef, chicken, pork, egg, and milk samples, as well as environmental, cloacal, and rectal swab-collected samples from supermarkets, open markets, abattoirs, and poultry, swine, and buffalo farms. Using the disc diffusion method, it was determined that 78.6% and 55.4% of the isolates were resistant to at least one antimicrobial and multiple drugs, respectively. In 141 isolates not susceptible to quinolones, 115 (81.6%) harbored quinolone-resistant determinants and had mutations predominantly in the quinolone-resistance determining regions (QRDRs) of gyrA and parC. Plasmid-mediated, quinolone resistance (PMQR) and Qnr family (qnrA1, qnrB4, and qnrS1) genes were detected in all isolates. Forty-eight sequence types were identified in isolates harboring mutations in QRDR and/or PMQR genes by multilocus sequence typing analysis. Moreover, 26 isolates harboring mutations in QRDR and/or PMQR genes belonged mostly to phylogroup B1 and Enteroaggregative E. coli. In conclusion, a high prevalence of E. coli was found in food-producing animals and products derived from them, which could potentially spread high-risk clones harboring quinolone-resistance determinants.

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“…Among the 35 stations, 16 stations harbored blaCTX-Mtypes of ESBL and higher number of ESBL were detected near the barrage region. reported in sewage and drinking water of Kerala and Maharastra in India (Salim et al 2019;Rayasam et al 2019).…”

Section: Genotyping Of E Coli By Eric-pcrmentioning

confidence: 99%

Predominance of genetically diverse ESBL Escherichia coli identified in resistance mapping of Vembanad Lake, the largest fresh-cum-brackishwater lake of India

Vaiyapuri

Sebastian

George

et al. 2021

Environ Sci Pollut Res

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Antimicrobial resistance (AMR) burden in Escherichia coli along the 90 km stretch of Vembanad Lake, Kerala, India was assessed. Seventy-seven percent of water samples drawn from 35 different stations of the Lake harbored E. coli. Antibiotic susceptibility test performed on 116 E. coli isolates revealed 81% were resistant to ≥ one antibiotic with 39 AMR profiles, 30% multidrug resistant, 32% extended spectrum  lactamase (ESBL) producers as per CLSI.The probability of isolating cefotaxime resistant E. coli was the highest 0.7 (P ≤ 0.05) in the Lake. Genetically diverse ESBL types blaTEM-116,blaCTX-M -152, blaCTX-M -27,blaCTX-M -55, blaCTX-M-205, and blaSHV-27 were identified. Molecular typing (ERIC PCR, MLST and PBRT) confirmed the diversity among E. coli between and within the stations. ST11439 and Single and Double Loci Variants of ST443, ST4533 were identified in Multi locus sequence typing (MLST) analysis. Inc plasmids (B/O, F, W, I1, FIIA, HI1, P-1, K/B and N) identified in the Lake evidences the transmission potential. Low multiple antibiotic resistance index (average < 0.2) indicating lower risk to the human population albeit, an emerging concern of ESBL resistance in the Lake. The occurrence of genetically variant ESBL E. coli in Vembanad Lake signals health hazards and necessitates pragmatizing strategic control measures.

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Draft Genome Sequence of an Escherichia coli Sequence Type 155 Strain Isolated from Sewage in Kerala, India

Cited by 9 publications

References 15 publications

Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria

Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria

Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines

Predominance of genetically diverse ESBL Escherichia coli identified in resistance mapping of Vembanad Lake, the largest fresh-cum-brackishwater lake of India

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