Draft Genome Sequence of an Acinetobacter Genomic Species 3 Strain Harboring a blaNDM-1 Gene

Chen, Yong; Cui, Yu‐Jun; Pu, Fei; Jiang, Guoqin; Zhao, Xiangna; Yuan, Yapeng; Zhao, Wei; Li, Dongfang; Liu, Hui; Li, Yin; Liang, Tiantian; Xu, Li; Wang, Yan; Song, Qing; Yang, Jiyong; Liang, Long; Yang, Ruifu; Han, Li; Song, Yajun

doi:10.1128/jb.06202-11

Cited by 15 publications

(13 citation statements)

References 17 publications

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“…In contrast to most other countries where NDM has been mainly identified in Enterobacteriaceae , in China to date NDM has only been reported in Acinetobacter [9], [11], [12], [24] and Enterococcus faecium [13]. However, other types of MBLs, such as IMP, and other classes of acquired carbapenemases, such a KPC, have been reported in Enterobacteriaceae in China[25]–[29], but isolates of NDM-1-producing E.coli have not previously been identified in China.…”

Section: Discussionmentioning

confidence: 66%

“…Although nosocomial transmission of NDM-1 has occurred in many countries[26], [32], [33], medical tourism plays a significant role in the spread of NDM-1[34], and traveling to the Indian subcontinent is a significant risk factor of infection with an NDM-1-producing bacterium[35]. Patients in China who were found to have bla NDM-1 -carrying bacteria had no history of traveling to the Indian subcontinent or another country[9], [11], [13], [24]. In this study, the patient carrying the bla NDM-1 -positive E. coli strain had no foreign travel history.…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, NDM-1-producing strains have been seen only in Acinetobacter spp. [9]–[12] and Enterococcus faecium[13] in China. There is no evidence of the emergence of NDM-1-producing Enterobacteriaceae in China at this point, although it was found in the stool samples of a 1-year-old infant and his mother in Hong Kong who once traveled to and were hospitalized in Hunan Province, China[14].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

Liu

Wang

et al. 2013

PLoS ONE

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New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla NDM-1 in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla NDM-1, bla CTX-M-57, and bla TEM-1 were identified in the isolate. bla NDM-1 was transferable to E. coli EC600 and DH5α in both plasmid conjugation experiments and plasmid transformation tests. BJ01 was identified as a new sequence type, ST224, by multilocus sequence typing. Analysis of genetic environment shows complex transposon-like structures surrounding the bla NDM-1 gene. Genetic analysis revealed that the region flanking bla NDM-1 was very similar to previously identified NDM-positive Acinetobacter spp. isolated in China. The findings of this study raise attention to the emergence and spread of NDM-1-carrying Enterobacteriaceae in China.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

Liu

Wang

et al. 2013

PLoS ONE

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“…However, the situation appears more complex, as in pNDM-BJ01 from Acinetobacter lwoffii (5), ISAba125 upstream of bla NDM-1 appears to be part of an ISAba125-mediated composite transposon (Tn125), flanked by 3-bp direct repeats (DRs), that has been inserted downstream of aphA6 in the context found in Tn5393d from Alcaligenes faecalis (6). In the closely related plasmids pNDM-BJ02 from A. lwoffii (5) and pAB_D499 from A. pittii (2), only a small fragment of ISAba125 remains downstream of bla NDM-1 , possibly due to recombination between two copies of a repeated short sequence. Tn125 flanked by different DRs has also been found on the A. baumannii chromosome (15,16).…”

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confidence: 99%

Genetic Contexts of bla _NDM-1

Partridge

Iredell

2012

Antimicrob Agents Chemother

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“…Our isolate was related to only one other A. pittii strain, TG6411, but with a lower score of 221. A total of 13 A. pittii draft genomes have been described in the BioProject (http://www.ncbi.nlm.nih.gov/bioproject/); however, draft ge-nomes of only three isolates were published, including one draft genome of an NDM-1-producing A. pittii strain from China (13).…”

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A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia

et al. 2015

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An IMP-4-producing Acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by rpoB and gyrB sequencing and multiplex-PCR-based analysis revealed that the isolate was A. pittii. Whole-genome sequencing of this A. pittii isolate determined the presence of bla OXA-96 , bla CARB-2 , and a novel bla OXA-421 gene. The position of this novel bla OXA-421 gene was similar to that of bla OXA-51 in A. baumannii, downstream of the phosphinothricin N-acetyltransferase gene and upstream of fxsA in the chromosome. This A. pittii isolate was found to belong to sequence type 119 (ST119). Here, we report the first isolation of IMP-4-producing A. pittii ST119 with a novel bla OXA-421 gene from a patient in Australia and characterize its draft genome. CASE REPORTA 67-year-old diabetic woman suffered a fall leading to a displaced distal spiral tibial plateau fracture. In the weeks prior to the fall, she had received multiple antimicrobials (clindamycin, lincomycin, cephalexin, ciprofloxacin, and ceftazidime) for an infected hematoma of the breast and a series of lower respiratory tract infections. The patient underwent definitive repair of the fracture but postoperatively developed osteomyelitis. Debridement of the leg wound was performed. Acinetobacter species and vancomycin-resistant Enterococcus strains were isolated from the tissue removed. This Acinetobacter species (CR12-42) was carbapenem resistant. Despite ongoing antibiotic treatment, the patient's leg required amputation in March 2013, after continuous inflammation, infections for more than 5 months, and an episode of severe Clostridium difficile infection resulting in colectomy. The leg infection was resolved by the amputation.The initial identification of this Acinetobacter species was done by Vitek 2. Antimicrobial susceptibility testing by Vitek 2 (bioMérieux) showed resistance to carbapenems, ceftazidime, ceftriaxone, cefepime, gentamicin, tobramycin, trimethoprimsulfamethoxazole, ticarcillin-clavulanic acid, and ciprofloxacin according to the EUCAST standard (1). The isolate was referred to our laboratory at the University of Queensland Centre for Clinical Research. The Acinetobacter isolate was identified to the species level by a gyrB multiplex PCR, which revealed that CR12-42 was Acinetobacter pittii (2). Partial rpoB sequencing (3) confirmed that CR12-42 was A. pittii.Phenotypic characterization to determine the class of carbapenemase was performed as previously described (4-6). The A. pittii isolate showed a metallo-␤-lactamase phenotype by producing a larger inhibition zone around carbapenem disks with EDTA than around carbapenem disks alone (Ͼ5-mm breakpoint increase in the size of the inhibition zone). The isolate also produced a positive result in the modified Hodge and Carba NP tests for carbapenemase production. MICs were determined with Etest (bioMérieux). The isolate was resistant to all of the carbapenems tested, i.e., ceftazidime, cefotaxime, cefepime, cefo...

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Draft Genome Sequence of an Acinetobacter Genomic Species 3 Strain Harboring a blaNDM-1 Gene

Cited by 15 publications

References 17 publications

Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

Genetic Contexts of bla _NDM-1

A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia

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Draft Genome Sequence of an Acinetobacter Genomic Species 3 Strain Harboring a blaNDM-1 Gene

Cited by 15 publications

References 17 publications

Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

Genetic Contexts of bla NDM-1

A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia

Contact Info

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Genetic Contexts of bla _NDM-1