Draft Genome Sequence of “
            <i>Candidatus</i>
            Phytoplasma pruni” (X-Disease Group, Subgroup 16SrIII-B) Strain ChTDIII from Argentina

Fernández, Franco Daniel; Zübert, Christina; Huettel, Bruno; Kube, Michael; Conci, Luis Rogelio

doi:10.1128/mra.00792-20

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…The first complete genome of phytoplasma (onion yellows phytoplasma mild strain (OY-M) was published in 2004 [ 6 ] (data deposited into GenBank in 2003). So far, 47 phytoplasma genomes (35 draft and 12 complete) have been sequenced involving 13 groups and 29 subgroups ( Figure 2 and Table 1 , [ 6 , 7 , 14 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ]). The size of the complete ge-nomes ranges from 576 to 960 Kb.…”

Section: Phytoplasma Nomenclature: Delineation Of Candidatu...mentioning

confidence: 99%

Phytoplasma Taxonomy: Nomenclature, Classification, and Identification

Wei

Zhao

2022

Biology

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Phytoplasmas are pleomorphic, wall-less intracellular bacteria that can cause devastating diseases in a wide variety of plant species. Rapid diagnosis and precise identification of phytoplasmas responsible for emerging plant diseases are crucial to preventing further spread of the diseases and reducing economic losses. Phytoplasma taxonomy (identification, nomenclature, and classification) has lagged in comparison to culturable bacteria, largely due to lack of axenic phytoplasma culture and consequent inaccessibility of phenotypic characteristics. However, the rapid expansion of molecular techniques and the advent of high throughput genome sequencing have tremendously enhanced the nucleotide sequence-based phytoplasma taxonomy. In this article, the key events and milestones that shaped the current phytoplasma taxonomy are highlighted. In addition, the distinctions and relatedness of two parallel systems of ‘Candidatus phytoplasma’ species/nomenclature system and group/subgroup classification system are clarified. Both systems are indispensable as they serve different purposes. Furthermore, some hot button issues in phytoplasma nomenclature are also discussed, especially those pertinent to the implementation of newly revised guidelines for ‘Candidatus Phytoplasma’ species description. To conclude, the challenges and future perspectives of phytoplasma taxonomy are briefly outlined.

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Section: Phytoplasma Nomenclature: Delineation Of Candidatu...mentioning

confidence: 99%

Phytoplasma Taxonomy: Nomenclature, Classification, and Identification

Wei

Zhao

2022

Biology

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“…P. meliae’ draft genome; Table S4: List of single-copy genes ID; Table S5: Genome-to-genome metrics. References [ 40 , 41 , 42 , 43 , 44 ] are cited in the supplementary materials.…”

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confidence: 99%

Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization

Fernández,

Yan,

Kuo

et al. 2024

Microorganisms

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‘Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing disease, which has become a major phytosanitary problem for chinaberry forestry production in Argentina. Despite its economic impact, no genome information of this phytoplasma has been published, which has hindered its characterization at the genomic level. In this study, we used a metagenomics approach to analyze the draft genome of the ‘Ca. P. meliae’ strain ChTYXIII. The draft assembly consisted of twenty-one contigs with a total length of 751.949 bp, and annotation revealed 669 CDSs, 34 tRNAs, and 1 set of rRNA operons. The metabolic pathways analysis showed that ChTYXIII contains the complete core genes for glycolysis and a functional Sec system for protein translocation. Our phylogenomic analysis based on 133 single-copy genes and genome-to-genome metrics supports the classification as unique ‘Ca. P. species’ within the MPV clade. We also identified 31 putative effectors, including a homolog to SAP11 and others that have only been described in this pathogen. Our ortholog analysis revealed 37 PMU core genes in the genome of ‘Ca. P. meliae’ ChTYXIII, leading to the identification of 2 intact PMUs. Our work provides important genomic information for ‘Ca. P. meliae’ and others phytoplasmas for the 16SrXIII (MPV) group.

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Draft genome sequence of Cicuta witches' broom phytoplasma, subgroup 16SrIII-J: a subgroup with phytopathological relevance in South America

Fernández,

Guzmán,

Conci

2024

Trop. plant pathol.

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Draft Genome Sequence of “ Candidatus Phytoplasma pruni” (X-Disease Group, Subgroup 16SrIII-B) Strain ChTDIII from Argentina

Abstract: Herein, we report the draft genome sequence of “Candidatus Phytoplasma pruni” strain ChTDIII (subgroup 16SrIII-B). The final assembly consists of 790,517 nucleotides organized in 67 contigs (minimal size, 1 kb), with a G+C content of 29.4% and encoding 672 proteins.

Cited by 3 publications

References 16 publications

Phytoplasma Taxonomy: Nomenclature, Classification, and Identification

Phytoplasma Taxonomy: Nomenclature, Classification, and Identification

Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization

Draft genome sequence of Cicuta witches' broom phytoplasma, subgroup 16SrIII-J: a subgroup with phytopathological relevance in South America

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