Draft Genome Sequence of 16SrIII-J Phytoplasma, a Plant Pathogenic Bacterium with a Broad Spectrum of Hosts

Zamorano, Alan; Fiore, Nicola

doi:10.1128/genomea.00602-16

Cited by 15 publications

(11 citation statements)

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“…So far, only one draft genome has been described for the subgroup 16SrIII-J (Zamorano and Fiore, 2016…”

Section: Detection and Characterization Of Effector Protein Sap54 Hommentioning

confidence: 99%

“…During the annotation process of the draft genome of the periwinkle associated 16SrIII-J phytoplasma, Zamorano and Fiore (2016) detected 11 CDSs containing the SEC translocase complex signal peptide, but the authors stated that they did not find any evidence of SAP11, SAP54, PHYL, or TENGU like genes. As these genes and their products appear to be fundamentals in the pathogenic process of phytoplasma infection, we embarked in an exhaustive exploration of their sequence dataset in order to assess the presence of signatures associated with these important genes.…”

Section: Identification Of Sap54 Homologue In 16sriii-j Phytoplasma Gmentioning

confidence: 99%

“…While five genomes of phytoplasmas have been reported based on traditional Sanger technologies (Oshima et al 2004;Bai et al 2006;Tran-Nguyen et al 2008, Kube et al 2008, Andersen et al 2013, since the implementation of next generation sequencing (NGS) methods, a rapid increase of available genome data has scaled. Since NGS, one additional complete genome has been depicted (Orlovskis et al 2017), and other 16 have been partially (draft) sequenced (Chung et al 2013;Mitrović et al 2014;Saccardo et al 2012, Fischer et al 2016Pacifico et al 2015;Zamorano and Fiore 2016;Sparks et al 2018). Analyzes of these genomes have shown, despite their small size, the phytoplasma genome contains a substantial number of genes in multiple copies (Bai et al 2006;Davis et al 2003;Jomantiene and Davis 2006;Oshima et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Within this group, phytoplasmas belonging to subgroup 16SrIII-J were associated to diverse diseases in Argentina, Chile and Brazil (Fernández et al 2017;Fugita et al 2017;Montano et al 2011 Pérez-López et al 2016;Quiroga et al 2017;Fernández et al 2018). Despite the growing importance of this particular subgroup of phytoplasmas, only one draft genome has been obtained so far (Zamorano and Fiore 2016). Moreover, there are no reports describing effector proteins in phytoplasmas from subgroup 16SrIII-J present in Argentina.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of effector protein SAP54 inBellis Virescence Phytoplasma(16SrIII-J)

Fernández

Debat

Conci

2018

Preprint

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Phytoplasmas are wall-less bacteria, with a parasitic life style responsible for numerous plant diseases worldwide. The genomic landscape of phytoplasmas has been scarcely studied. Nevertheless, since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasmas genomes are available in public databases. In South America, phytoplasmas from 16SrIII group (X-disease) are the most widely distributed, and only a draft genome from a phytoplasmas 16SrIII-J infected periwinkle from Chile has been generated (Phytoplasma Vc33). Here, in grafting experiments we characterized the phenotypic signatures of an Argentinian daisy derived isolate of a 16SrIII-J phytoplasma (Bellis Virescence Phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein of Bellis Virescence Phytoplasma. Structural and phylogenetic analyses suggested that the identified SAP54 is highly conserved, and that its co-divergence among phytoplasma is not directly consistent with the evolutionary trajectories derived from rRNA analyses. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual influence in pathogenesis.

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“…So far, only one draft genome has been described for the subgroup 16SrIII-J (Zamorano and Fiore, 2016…”

Section: Detection and Characterization Of Effector Protein Sap54 Hommentioning

confidence: 99%

Section: Identification Of Sap54 Homologue In 16sriii-j Phytoplasma Gmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterization of effector protein SAP54 inBellis Virescence Phytoplasma(16SrIII-J)

Fernández

Debat

Conci

2018

Preprint

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“…For immunodominant proteins, we designed specific primers based on the genomic information available in public database. So far, only one draft genome has been described for subgroup 16SrIII-J [26] which belongs to Vc33 phytoplasma isolate from periwinkle. Annotation pipeline led us to identify imp and idpA gene sequences [27].…”

mentioning

confidence: 99%

‘Candidatus Phytoplasma platensis’, a novel taxon associated with daisy (Bellis perennis) virescence and related diseases in South America

Fernández

Galdeano

2019

Preprint

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Bellis perennis virescence (BellVir) phytoplasma affects ornamental daisies in Argentina. It has been previously classified within the X-disease group, subgroup III-J, which is one of the most important and widely distributed in South America, affecting diverse plant hosts. In this study, we compared 16S rRNA, ribosomal proteins rpIV and rps3, secA and immunodominant proteins imp and idpA genes of BellVir phytoplasma with previously described ‘Candidatus Phytoplasma’ species. The 16S rRNA gene of strain BellVir shared less than 97.5% with all previously described ‘Ca. Phytoplasma’ taxa except for ‘Ca. Phytoplasma pruni’. According to the recommended rules for the description of novel taxa within ‘Ca. Phytoplasma’, it should be considered as ‘Ca. P. pruni’-related strain. However, multilocus analysis showed further molecular diversity that distinguished BellVir phytoplasma from ‘Ca. Phytoplasma pruni’. Besides, BellVir phytoplasma and 16SrIII-J related strains have a geographical distribution restricted to South America, where ‘Ca. P.pruni’ has not been detected. Two insect vectors have been reported to transmit 16SrIII-J phytoplasmas, which have not been found to transmit ‘Ca. Phytoplasma pruni’. Having a wide host range, they have not been detected in Prunus persica. Therefore, based on multilocus sequence analyses, specific vector transmission and geographical distribution, we propose the recognition of the novel phytoplasma species ‘Ca. Phytoplasma platensis’, within the X-disease clade, with Bellis perennis virescence phytoplasma as the reference strain.

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Phytoplasmas: An Update

Bertaccini

Lee

2018

Phytoplasmas: Plant Pathogenic Bacteria - I

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Draft Genome Sequence of 16SrIII-J Phytoplasma, a Plant Pathogenic Bacterium with a Broad Spectrum of Hosts

Cited by 15 publications

References 11 publications

Molecular characterization of effector protein SAP54 inBellis Virescence Phytoplasma(16SrIII-J)

Molecular characterization of effector protein SAP54 inBellis Virescence Phytoplasma(16SrIII-J)

‘Candidatus Phytoplasma platensis’, a novel taxon associated with daisy (Bellis perennis) virescence and related diseases in South America

Phytoplasmas: An Update

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