Draft Genome Sequence and Annotation of the Synthetic Textile Dye-Decolorizing Strain Bacillus amyloliquefaciens AD20, Isolated from a Dye Waste Pond

Abstract

A limited number of Bacillus amyloliquefaciens genome sequences have been generated and are available in the public domain from soil, fermented foods, and plants. Here, we report the whole-genome sequence of B. amyloliquefaciens AD20, isolated from a dye pond with azo dye-decolorization capabilities.

Draft Genome Sequence and Annotation of Priestia aryabhattai Strain BD1 Isolated from a Dye Sediment

“…A dye waste sediment sample was collected behind a textile company in Lagos, Nigeria, to explore for azo dye-degrading bacteria. To isolate indigenous bacteria, 10-fold serial dilutions of up to 10 −6 of the sediment sample were prepared and grown on tryptic soy agar plates and incubated at 37°C for 18 h. Pure cultures of unique isolates that were screened from the total culturable bacteria were individually tested on minimal salts medium infused with Cibacron dyes—reactive blue 4% red (RBFR) and brilliant yellow 6% green (CYPGS) ( 5 ). The Cibacron dyes were provided by the textile company.…”

“…Genomic DNA was isolated from cells of P . aryabhattai grown overnight at 37°C in tryptic soy broth (TSB) using the Zymo ZR Quick-DNA fungal/bacterial miniprep ( 5 ). The Illumina NextSeq 500 sequencing (2- by 150-bp long paired-end) was done at Quadram Institute Bioscience, Norwich, United Kingdom.…”