Draft genome and description of Consotaella salsifontis gen. nov. sp. nov., a halophilic, free-living, nitrogen-fixing alphaproteobacterium isolated from an ancient terrestrial saline spring

Abstract

A free-living, nitrogen-fixing, mesophilic and facultative aerobe, designated strain USBA 369, was isolated from a terrestrial saline spring of the Colombian Andes. The non-sporulating rods (1.5×0.8 µm) with rounded ends stained Gram-negative and were motile by means of lophotrichous flagella. The strain grew optimally at 30 °C, at pH 6.9-7.5 and with 1.5 % (w/v) NaCl. The major fatty acids detected were C18 : 1ω7c and C19 : 0 cyclo ω8c, and the respiratory lipoquinone ubiquinone 10 (Q-10) was present. The genome consisted of 4.65 Mb with a DNA G+C content of 64.3 mol%. A total of 4371 genes were predicted and, of those, 4300 were protein coding genes and 71 were RNA genes. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain USBA 369 formed a different lineage within the class Alphaproteobacteria, order Rhizobiales, and DNA homology studies with the most closely related genera, Aurantimonas, Aureimonas and Rhizobium (95 % 16S rRNA gene sequence similarity), showed values of <15 %. The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain USBA 369 and the closely related genera. On the basis of the phenotypic, chemotaxonomic and phylogenomic evidence, strain USBA 369 is considered to represent a novel genus and a novel species for which the name Consotaella salsifontis gen. nov., sp. nov. is proposed. The type strain is USBA 369 (=KCTC 22549=CMPUJ U369).

Rational Design of Aptamer-Tagged tRNAs

“…It is well known that a large subgroup of α-Proteobacteria has a non-canonical tRNA His lacking the G-1:C73 pair and has a non-canonical HisRS species that recognizes the non-canonical G1:U72 pair and A73 in addition to the GUG anticodon [ 17 , 57 , 58 , 59 , 60 ]. The two α-proteobacterial species are Afifella pfennigii DSM 17,143 ( Ap ) [ 17 ] and Consotaella salsifontis USBA 369 ( Cs ) [ 61 ], because the well-studied α-proteobacterial Caulobacter crescentus HisRS was not active enough in E. coli [ 60 ]. Two kinds of allo-tRNA chassis, S072 and S005, were engineered to have a typical α-proteobacterial tRNA His anticodon arm as their V-arms, to develop H072-αψHis-3 and αH005-αψHis-3/4/5 variants ( Figure 7 A).…”

Antarcticirhabdus aurantiaca gen. nov., sp. nov., isolated from Antarctic gravel soil

“…The major named fatty acids are usually C 18 : 1 ω7 c and C 19 : 0 cyclo ω 8 c [2]. Members of the family Aurantimonadaceae have been isolated from various environments such as offshore surface seawater [3], deep-sea sediment [4], water-cooling system [5], surface-sterilized root of a halophyte [6], terrestrial saline spring [7], deep-sea hydrothermal vent plume [8] and poplar tree bark [9]. This study focuses on the description of a novel genus and species with the type strain R10 T was isolated from Antarctic gravel soil.…”

Salifodinibacter halophilus gen. nov., sp. nov., a halophilic gammaproteobacterium in the family Salinisphaeraceae isolated from a salt mine in the Colombian Andes

“…The morphology and motility of strains USBA 874 and USBA 960 T were determined by phase contrast microscopy (Eclipse 50i, Nikon) and by electron microscopy (transmission electron microscopy and scanning electron microscopy) for strain USBA 960 T . Negative staining, preparation of thin sections for electron microscopy and electron microscope examination were performed as described by Diaz-Cárdenas et al [20]. Cells of strains USBA 874 and USBA 960 T occurred singly or in pairs and were straight, short bacilli measuring 0.9-1.5 µm long and 0.3-0.6 µm wide with rounded ends and stained Gram-negative ( Fig.…”

“…Negative staining, preparation of thin sections for electron microscopy and electron microscope examination were performed as described by Diaz-Cárdenas et al . [20]. Cells of strains USBA 874 and USBA 960 T occurred singly or in pairs and were straight, short bacilli measuring 0.9–1.5 µm long and 0.3–0.6 µm wide with rounded ends and stained Gram-negative ().…”