DPPH Radical Scavenging Activity and Polyphenols in the Pods of 3 Common Beans

Ci, Zhaohong; Jiang, Chengyu; Tsukamoto, Chigen; Kojima, Masayasu

doi:10.12691/jfnr-5-12-4

Cited by 5 publications

(7 citation statements)

References 17 publications

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“…SRB (purple) showed a higher polyphenol content and DPPH radical scavenging activity than those of SRB (black) (p<0.05). A positive correlation between the polyphenol content and DPPH radical scavenging activity for common beans has been reported by Ci et al [14]. Polyphenols from the coat and cotyledon of SRB (purple) and SRB (black) were analyzed.…”

Section: Discussionmentioning

confidence: 53%

Suppressive Effect of Polyphenols from the Seed Coat of Scarlet Runner Beans on Blood Glucose Levels

Ci¹,

Jiang²,

Kojima³

2018

JFNR

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Scarlet runner beans (SRB) are a valuable source of many nutrients, including proteins, starch, dietary fiber, and oligosaccharides, and are used in various foods in Japan. In this study, we analyzed their polyphenol and procyanidin contents, DPPH radical scavenging activity, and reducing power. SRB (purple) had a higher polyphenol content and DPPH radical scavenging activity than those of SRB (black). The reducing power of SRB (black) (5.1 mg/g) was greater than that of SRB (purple) (4.1 mg/g). Both SRB (purple) and SRB (black) had greater levels of polymeric polyphenols (total Fra.II and Fra.III) than monomeric polyphenols (Fra.I), and inhibited the activity of α-glucosidase in a dose-dependent manner. SRB (black) showed higher α-glucosidase inhibitory activity (IC 50 , 26.4 µg/mL) than that of SRB (purple) (IC 50 , 39.7 µg/mL), and a mixed pattern of inhibition (non-competitive and uncompetitive). The α-glucosidase inhibitory activity was greater for polyphenols from the seed coat (>91%) than from the cotyledon (<0.1%) for SRB (purple) and SRB (black). Both 250 mg/kg and 750 mg/kg polyphenols from the coat of SRB (purple) effectively suppressed the elevation of blood glucose levels after the oral administration of starch in mice. These results suggest that the seed coat of SRB has useful properties and, in particular, has potential applications for the treatment of diabetes.

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Section: Discussionmentioning

confidence: 53%

Suppressive Effect of Polyphenols from the Seed Coat of Scarlet Runner Beans on Blood Glucose Levels

Ci¹,

Jiang²,

Kojima³

2018

JFNR

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“…We found positive relationship between polyphenol content and DPPH radical scavenging activity in peel, core and pulp of five pear cultivars, and the correlation coefficient was 0.98. Many studies have demonstrated that polyphenols possessed strong antioxidant activity [18,19,20].…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Antioxidant and α-Glucosidase Inhibitory Activity of Polyphenols in Peel and Core from Five Different Pear Varieties

Ci¹,

Kojima²

2018

JFNR

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This study was performed to compare polyphenol content, DPPH radical scavenging activity and αglucosidase inhibitory activity in peel, core and pulp of five different pear cultivars. Oligomeric polyphenols were the main polyphenols in core, which exhibited greater polyphenols, DPPH radical scavenging activity, and αglucosidase inhibitory activity, compared to peel and pulp. For the peel, Japanese pears including Yasato, 20-Seki and Shinko pear contained relatively more polyphenol content and α-glucosidase inhibitory activity, compared to European pears including Starkrimson and Purekosu pear. Monomeric polyphenols were the main polyphenols in the peel of European pears, in contrast, oligomeric polyphenols were the main polyphenols in those of Japanese pears. The peel of Yasato pear had high ratios of non-monomeric polyphenols, and showed highest DPPH radical scavenging activity and α-glucosidase inhibitory activity amongst five pear cultivars. Thus DPPH radical scavenging activity and α-glucosidase inhibitory activity may be related with non-monomeric polyphenols in pears. Moreover, as comparing with peel, pulp had lower polyphenol content, DPPH radical scavenging activity and α-glucosidase inhibitory activity. All of the observations suggested that peel and core of pear contained abundant polyphenols and possess potential the values of reusing.

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“…Thus, we speculate that the DPPH radical scavenging activity of these polyphenols are mainly related to non-monomeric polyphenols. Notably, we previously reported that polymeric polyphenols may possess greater antioxidant activity [16].…”

Section: Polyphenol Content Dpph Radical Scavenging Activity and Pomentioning

confidence: 99%

Suppressive Effect of Polyphenols from Immature Pear Fruits on Blood Glucose Levels

Ci¹,

Jiang²,

Cui³

et al. 2018

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The agricultural practice of pear thinning involves the removal of immature pear fruits, which are usually treated as waste but can contain high levels of beneficial polyphenols. To assess whether these fruits provide commercial utility, we aimed to examine the inhibitory effects of polyphenols from the immature pear fruits of the Hosui and Kosui cultivars on α-glucosidase in vitro and blood glucose levels in mice after the oral administration of starch. Polyphenols were extracted from the immature pear fruits by 80% ethanol and 70% acetone, and the mixed extraction was purified by HP-20 column. The polyphenol contents of the Hosui and Kosui immature pear fruits were 28.5 and 20.9 mg/g D.W., respectively, and both were characterized by high percentages of oligomeric and polymeric polyphenols. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of the polyphenols from Hosui and Kosui fruits were 115.8 and 76.3 μmol/g D.W., respectively, indicating a significant difference between the two cultivars. Moreover, compared with the polyphenols from immature Kosui fruits, which had an IC 50 value of 66.4 μg/mL, those from Hosui fruits, with an IC 50 value of 21.3 μg/mL, resulted in more potent inhibition of α-glucosidase activity. Polyphenols were fractionated with a Sephadex LH-20 column into fraction I (Fra.I; monomeric), fraction II (Fra.II; oligomeric), and fraction III (Fra.III; polymeric). Compared with Fra.I, Fra.II and Fra.III from immature Hosui and Kosui fruits showed higher inhibition of α-glucosidase activity, with the highest inhibitory activity in Fra.III. Thus, α-glucosidase inhibitory activity appears to be related to the degree of polyphenol polymerization. Moreover, polyphenols from immature Hosui fruits effectively suppressed increases in blood glucose levels after the oral administration of starch in mice. These results suggest that the immature pear fruits of the Hosui and Kosui cultivars have useful properties and, in particular, potential applications for the treatment of diabetes.

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DPPH Radical Scavenging Activity and Polyphenols in the Pods of 3 Common Beans

Cited by 5 publications

References 17 publications

Suppressive Effect of Polyphenols from the Seed Coat of Scarlet Runner Beans on Blood Glucose Levels

Suppressive Effect of Polyphenols from the Seed Coat of Scarlet Runner Beans on Blood Glucose Levels

Antioxidant and α-Glucosidase Inhibitory Activity of Polyphenols in Peel and Core from Five Different Pear Varieties

Suppressive Effect of Polyphenols from Immature Pear Fruits on Blood Glucose Levels

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