Doxycycline suppresses Chlamydia pneumoniae induced interferon-gamma responses in peripheral blood mononuclear cells in children with allergic asthma

Smith‐Norowitz, Tamar A.; Weaver, Diana; Norowitz, Yitzchok M.; Hammerschlag, Margaret R.; Joks, Rauno; Durkin, Helen G.; Kohlhoff, Stephan

doi:10.1016/j.jiac.2018.02.004

Cited by 8 publications

(18 citation statements)

References 29 publications

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“…Total serum IgE levels were determined in serum using the UniCap Total IgE fluoroenzyme immunoassay (Pharmacia and Upjohn Diagnostics, Freiburg, Germany) as previously described [14]. Tests were performed in the Clinical Diagnostic Laboratory at SUNY Downstate Medical Center (Brooklyn, NY).…”

Section: Immunoglobulin Determination: Total Serum Igementioning

confidence: 99%

“…PBMC were separated from blood on a Ficoll-Paque (GE Healthcare, Sweden) gradient (density 1.077) and put into cell culture a previously described [14], at 37˚C in cRPMI medium in a humidified 5% CO 2 atmosphere for up to 10 days. Cell viability was determined at 0, 48 and 240 hrs (>98%, 95%, and 90%, respectively), in the absence of any infection with C. pneumoniae.…”

Section: Cell Culturesmentioning

confidence: 99%

“…Following a 2 hr incubation to allow adherence, PBMC cultures were infected with C. pneumoniae (by adding purified EB for 1hr), or mock-infected (MI) and/or stimulated in the presence or absence of azithromycin (0.1 or 1.0 ug/mL) (Sigma) for either 48 hrs (IL-4) or up to 10 days (IgE) at 37˚C in cRPMI in a humidified 5% CO 2 atmosphere, as previously described [14]. All antibiotics were serially diluted (1:1, 1:2, 1:4, 1:10) [13] to determine optimal dose and kinetics [13], for suppression (for the purpose of cytokine production).…”

Section: In Vitro Infection With C Pneumoniae and Treatment With Antmentioning

confidence: 99%

“…For the in vitro quantitative determination of human cytokine or IgE content in cell culture supernatants, solid-phase sandwich ELISA assays were performed using either cytokine (IL-4: IL-4 Human ELISA kit, Thermo Fisher Scientific, Waltham, MA) or IgE ELISA test kits (Bio Quant, San Diego, CA), according to the manufacturer's recommended procedure, as previously described [14]. Cell culture supernatants were collected at either 48 hr p.i.…”

Section: Cytokine (Il-4) or Ige Determination: Elisamentioning

confidence: 99%

“…Smith-Norowitz et al recently reported that doxycycline suppressed C. pneumoniae induced interferon-gamma responses in PBMC in asthmatic children [14]. However, IL-4 levels did not significantly decrease after addition of ciprofloxacin (0.1 μg/ml) or azithromycin (1.0 μg/ml); C. pneumoniae infection and/or antibiotic treatment had no effect on IgE production in vitro [14].…”

Section: Introductionmentioning

confidence: 99%

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Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses

et al. 2020

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Background Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. Objective We previously demonstrated that doxycycline suppresses C. pneumoniae-induced production of IgE and IL-4 responses in peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Whereas macrolides have anti-chlamydial activity, their effect on in vitro antiinflammatory (IgE) and IL-4 responses to C. pneumoniae have not been studied. Methods PBMC from IgE-adult atopic subjects (N = 5) were infected +/-C. pneumoniae BAL69, +/azithromycin (0.1, 1.0 ug/mL) for 10 days. IL-4 and IgE levels were determined in supernatants by ELISA. IL-4 and IgE were detected in supernatants of PBMC (day 10). Results When azithromycin (0.1, 1.0 ug/ml) was added, IL-4 levels decreased. At low dose, IgE levels increased and at high dose, IgE levels decreased. When PBMC were infected with C. pneumoniae, both IL-4 and IgE levels decreased. Addition of azithromycin (0.1, 1.0 ug/mL) decreased IL-4 levels and had no effect on IgE levels. Conclusions These findings indicate that azithromycin decreases IL-4 responses but has a bimodal effect on IgE responses in PBMC from atopic patients in vitro.

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Section: Immunoglobulin Determination: Total Serum Igementioning

confidence: 99%

Section: Cell Culturesmentioning

confidence: 99%

Section: In Vitro Infection With C Pneumoniae and Treatment With Antmentioning

confidence: 99%

Section: Cytokine (Il-4) or Ige Determination: Elisamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses

et al. 2020

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Immunomodulation by Tetracyclines in the Critically Ill: An Emerging Treatment Option?

Sauer

Putensen

Bode

2022

Annual Update in Intensive Care and Emergency Medicine

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Fatal attractions that trigger inflammation and drive atherosclerotic disease

Sharma,

Mossman,

Austin

2024

Eur J Clin Investigation

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BackgroundAtherosclerosis is the salient, underlying cause of cardiovascular diseases, such as arrhythmia, coronary artery disease, cardiomyopathy, pulmonary embolism and myocardial infarction. In recent years, atherosclerosis pathophysiology has evolved from a lipid‐based to an inflammation‐centric ideology.MethodsThis narrative review is comprised of review and original articles that were found through the PubMed search engine. The following search terms or amalgamation of terms were used: “cardiovascular disease,” “atherosclerosis,” “inflammation,” “GRP78,” “Hsp60,” “oxidative low‐density lipoproteins,” “aldehyde dehydrogenase,” “β2‐glycoprotein,” “lipoprotein lipase A,” “human cytomegalovirus.” “SARS‐CoV‐2,” “chlamydia pneumonia,” “autophagy,” “thrombosis” and “therapeutics.”ResultsEmerging evidence supports the concept that atherosclerosis is associated with the interaction between cell surface expression of stress response chaperones, including GRP78 and Hsp60, and their respective autoantibodies. Moreover, various other autoantigens and their autoantibodies have displayed a compelling connection with the development of atherosclerosis, including oxidative low‐density lipoproteins, aldehyde dehydrogenase, β2‐glycoprotein and lipoprotein lipase A. Atherosclerosis progression is also concurrent with viral and bacterial activators of various diseases. This narrative review will focus on the contributions of human cytomegalovirus as well as SARS‐CoV‐2 and chlamydia pneumonia in atherosclerosis development. Notably, the interaction of an autoantigen with their respective autoantibodies or the presence of a foreign antigen can enhance inflammation development, which leads to atherosclerotic lesion progression.ConclusionWe will highlight and discuss the complex role of the interaction between autoantigens and autoantibodies, and the presence of foreign antigens in the development of atherosclerotic lesions in relationship to pro‐inflammatory responses.

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Doxycycline suppresses Chlamydia pneumoniae induced interferon-gamma responses in peripheral blood mononuclear cells in children with allergic asthma

Cited by 8 publications

References 29 publications

Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses

Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses

Immunomodulation by Tetracyclines in the Critically Ill: An Emerging Treatment Option?

Fatal attractions that trigger inflammation and drive atherosclerotic disease

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