Doxycycline Inducible Melanogenic Vaccinia Virus as Theranostic Anti-Cancer Agent

Kirscher, Lorenz; Deán‐Ben, Xosé Luís; Scadeng, Miriam; Zaremba, Angelika; Zhang, Qian; Kober, Christina; Fehm, Thomas Felix; Razansky, Daniel; Ntziachristos, Vasilis; Stritzker, Jochen; Szalay, Aladar A.

doi:10.7150/thno.12533

Cited by 19 publications

(17 citation statements)

References 33 publications

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“…Our approach can effectively enhance the FOV and hence the region where fish motion can be tracked in three dimensions. Optimal light delivery can further facilitate more accurate identification and quantification of bio‐markers in tumors visualized with volumetric OA tomography . Homogenous light delivery would also aid functional studies which rely upon spectral unmixing in multispectral OA tomography.…”

Section: Discussionmentioning

confidence: 99%

Uniform light delivery in volumetric optoacoustic tomography

Larney

Rebling

Chen

et al. 2019

Journal of Biophotonics

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Accurate image reconstruction in volumetric optoacoustic tomography implies the efficient generation and collection of ultrasound signals around the imaged object. Non‐uniform delivery of the excitation light is a common problem in optoacoustic imaging often leading to a diminished field of view, limited dynamic range and penetration, as well as impaired quantification abilities. Presented here is an optimized illumination concept for volumetric tomography that utilizes additive manufacturing via 3D printing in combination with custom‐made optical fiber illumination. The custom‐designed sample chamber ensures convenient access to the imaged object along with accurate positioning of the sample and a matrix array ultrasound transducer used for collection of the volumetric image data. Ray tracing is employed to optimize the positioning of the individual fibers in the chamber. Homogeneity of the generated light excitation field was confirmed in tissue‐mimicking agar spheres. Applicability of the system to image entire mouse organs ex vivo has been showcased. The new approach showed a clear advantage over conventional, single‐sided illumination strategies by eliminating the need to correct for illumination variances and resulting in enhancement of the effective field of view, greater penetration depth and significant improvements in the overall image quality.

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Section: Discussionmentioning

confidence: 99%

Uniform light delivery in volumetric optoacoustic tomography

Larney

Rebling

Chen

et al. 2019

Journal of Biophotonics

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“…In addition, although it has been possible to monitor the growth of Tyr -expressing tumours over periods of several weeks [241], extensive melanin production is generally associated with increased cytotoxicity [242]. A viable alternative is the tetracycline-regulated inducible system, which further enables versatile control of gene expression [243, 244]. Another promising enzymatic product that can generate optoacoustic signals is the bacterial pigment Violacein [245], which results from the multi-enzyme-based oxidation pathway of L-tryptophan (Fig.…”

Section: Dynamic Contrast Enhancement Approachesmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

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Visualization of dynamic functional and molecular events in an unperturbed in vivo environment is essential for understanding the complex biology of living organisms and of disease state and progression. To this end, optoacoustic (photoacoustic) sensing and imaging have demonstrated the exclusive capacity to maintain excellent optical contrast and high resolution in deep-tissue observations, far beyond the penetration limits of modern microscopy. Yet, the time domain is paramount for the observation and study of complex biological interactions that may be invisible in single snapshots of living systems. This review focuses on the recent advances in optoacoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches that enable new types of multiscale dynamic observations not attainable with other bio-imaging modalities. A wealth of investigated new research topics and clinical applications is further discussed, including imaging of large-scale brain activity patterns, volumetric visualization of moving organs and contrast agent kinetics, molecular imaging using targeted and genetically expressed labels, as well as three-dimensional handheld diagnostics of human subjects.

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“…Indeed, the delivery of low molecular weight therapeutics such as porphyrins (capable of generating singlet oxygen upon illumination) facilitates the application of such nanoscale assemblies for photodynamic therapy for the treatment of cancers or pathogens . Likewise, it is possible to deliver drugs to specific tissues (e.g., liver, colon including tissues that are typically challenging to deliver drugs to (e.g., across the blood‐brain barrier highlighting their prospects for use as theranostic agents (i.e., agents capable of diagnosis of a condition and its treatment) …”

Section: Poly(amino Acid) Assembliesmentioning

confidence: 99%