Doxycycline in Human Oviduct Mucosa and Plasma 24–30 Hours After an Oral Dose of 100 Mg

Abstract

Oviductal and peripheral plasma concentrations 24 hours after the last dose of Doxycycline (DC) were studied in 17 patients. The concentration of DC in healthy oviduct mucosa was 1.3 micrograms/g (range 0.6-1.7) and 1.1 micrograms/ml (range 0.6-1.7) in plasma. One patient with severe chronic salpingitis in acute exacerbation showed 3.6 micrograms/g in tissue and 2.4 micrograms/ml in plasma in proliferative phase.

Preoperative treatment with doxycycline reduces aortic wall expression and activation of matrix metalloproteinases in patients with abdominal aortic aneurysms

“…Also, stimulation by phorbol ester reflects one of the dominant molecular pathways acting to induce MMP-9 transcription in monocytic cells (ie, activation of protein kinase C and increased promoter activity regulated by activator protein-1 complexes). 71 Thus, the demonstration that doxycycline suppresses phorbol-stimulated expression of MMP-9 in THP-1 cells, particularly at concentrations typically achieved in tissue during systemic antibiotic treatment (4 to 20 µmol/L), 46,56,57 supports the likelihood that this is one of the mechanisms by which doxycycline regulates MMP-9 expression in human AAAs in vivo. Experiments in progress will help determine whether the molecular mechanisms of this effect reside in a suppression of MMP-9 transcription or mRNA stability, whether this mode of regulation involves alterations in specific signal transduction pathways or transcriptional elements, and how doxycycline influences other metalloproteinases and inhibitors normally expressed by mononuclear phagocytes.…”

“…THP-1 cells were preconditioned with doxycycline for 24 hours at 0, 2.5, 5, 10, and 12 µmol/L, a range of concentrations corresponding to tissue drug levels measured during therapy. 46,56,57 In the continuing presence of doxycycline, the cells were then exposed to 100 nmol/L PMA, an agent known to stimulate MMP-9 production in mononuclear phagocytes. [58][59][60] At all concentrations of doxycycline tested, THP-1 cells exhibited a normal morphologic appearance after PMA stimulation, with differentiation to an adherent phenotype and cell spreading on the substratum (data not shown).…”