Doxorubicin-loaded pH-sensitive polymeric blends for synergistic cancer treatment

Abstract

An intelligentized system that can transport chemotherapeutics to targets in tumors is an attractive strategy to reverse drug resistance in tumor cells. In this work, a doxorubicin (DOX) delivery system composed of PEGylated resveratrol (PEG-GLY-RES) and poly(histidine)-PEG (PEG-PHIS) was prepared, characterized and evaluated for synergistic cancer therapy. RES was chemically linked with PEG as a component and DOX was loaded into the polymeric blends. This system had an average size of 80 nm, and showed the desired pH-dependent drug release properties, with much faster drug release at pH 5.5, mimicking the tumoral microenvironment. The MTT assay showed the drug loaded blends had higher cytotoxicity than free DOX in all doses. Confocal laser scanning microscopy observation and flow cytometry analyses indicated that the blends were efficiently internalized into HeLa cells, released DOX into the cytoplasm, and entered the nuclei. Endocytosis inhibition results proved that the blends entered the HeLa cells mainly through clathrin-mediated endocytosis and macropinocytosis. It has been shown that such co-delivery of RES and DOX is promising for improvement of anti-cancer efficacy.Scheme 1 Drug loaded polymeric blends as emerging controlled drug release systems.31168 | RSC Adv., 2016, 6, 31167-31176This journal is

Combination Loading of Doxorubicin and Resveratrol in Polymeric Micelles for Increased Loading Efficiency and Efficacy

“…RSV can act as a chemosensitizer, providing a synergistic effect against cancer cells in vitro and in vivo. − The cardioprotective properties of RSV have been largely attributed to reduced oxidative stress, the inhibition of apoptosis, and modulated autophagy process . Research has been moving toward the combined loading of DOX and RSV into nanoparticles for controlled delivery to mitigate toxic side effects as well as cancer cell resistance to DOX, but few drug delivery systems have been reported. , Combination drug delivery using DOX and RSV shows an encouraging new path toward more efficient cancer treatment by combining polyphenols with anticancer drugs which have the potential to increase loading in these systems through hydrogen bonding and π-stacking interactions.…”

“…6 Research has been moving toward the combined loading of DOX and RSV into nanoparticles for controlled delivery to mitigate toxic side effects as well as cancer cell resistance to DOX, but few drug delivery systems have been reported. 20,21 Combination drug delivery using DOX and RSV shows an encouraging new path toward more efficient cancer treatment by combining polyphenols with anticancer drugs which have the potential to increase loading in these systems through hydrogen bonding and π-stacking interactions.…”

Gold nanoflowers modified ITO glass as SERS substrate for carbon tetrachloride-induced acute liver injury in vitro detection

“…[26][27][28] Raman spectroscopy is widely used to provide chemical and physical information based on vibrations in molecules. [33][34][35][36] In our previous work, we establish an ultrasensitive GST activity detection method, which can detect the SERS signals in PBS buffer. [33][34][35][36] In our previous work, we establish an ultrasensitive GST activity detection method, which can detect the SERS signals in PBS buffer.…”