Doxorubicin-Loaded Iron Oxide Nanoparticles Induce Oxidative Stress and Cell Cycle Arrest in Breast Cancer Cells

Abstract

Cancer is one of the most common diseases nowadays and derives from the uncontrollable growth of a single cell. Magnetic nanoparticles (NpMag) offer various possibilities for use in the biomedical area, including drug delivery mediated by magnetic fields. In the current study, we evaluated the in vitro effects of iron-oxide magnetic nanoparticles conjugated with the antitumor drug doxorubicin (Dox) on human breast cancer cells. Our results revealed that magnetic nanoparticles with Dox (NpMag+Dox) induce cellular redox imbalance in MCF-7 cells. We also demonstrate that iron-oxide nanoparticles functionalized with Dox induce oxidative stress evidenced by DNA damage, lipid peroxidation, cell membrane disruption, and loss of mitochondria potential. As a result, NpMag+Dox drives MCF-7 cells to stop the cell cycle and decrease cell migration. The association of NpMg+Dox induced a better delivery of Dox to MCF cells, mainly in the presence of a magnetic field, increasing the death of MCF cells which might reduce the toxicity for healthy cells providing a better efficacy for the treatment. Thus, iron-oxide nanoparticles and doxorubicin conjugated may be candidate for anticancer therapy.

pH-Responsible Doxorubicin-Loaded Fe3O4@CaCO3 Nanocomposites for Cancer Treatment

“…The DOX loading on nanoparticles’ surface serves as a prospective system that reduces side effects and avoids drug resistance problems [ 41 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ]. Therefore, it is essential to develop new pH-stimuli-responsive DOX-released multifunctional constructions based on MNCs [ 66 , 67 , 68 ]. Moreover, for further in vivo studies, the excellent capacity of DOX is required.…”

The Therapeutic Effects of MUC1-C shRNA@Fe3O4 Magnetic Nanoparticles in Alternating Magnetic Fields on Triple-Negative Breast Cancer

“…The anti-tumor effects of MNPs under magnetic fields have been reported in previous studies. For example, Hernandes et al designed doxorubicin-loaded magnetic nanoparticles, and they found the nanoparticles could inhibit the growth as well as migration of MCF-7 cells; 37 Meenakshi et al designed polypropylene sulfide-coated MNPs with good biocompatible and therapeutic efficacy for cancer treatment. 38 In the current work, we analyzed the therapeutic effects of the MUC1-C shRNA@Fe 3 O 4 MNPs on TNBC under the effect of AMF.…”

Advancing MRI with magnetic nanoparticles: a comprehensive review of translational research and clinical trials

“…Within these platforms, the inherent imaging attributes of magnetic NPs are coupled with various therapeutic strategies, thereby synergistically contributing to the mitigation of the pathological condition. This is the case in a vast array of diseases where clinical and preclinical studies are underway and magnetic NPs show a great deal of promise: brain cancer, especially gliomas, [157][158][159] breast cancer, 160,161 prostate cancer, 162,163 ovarian cancer, 164,165 cervical cancer, [166][167][168] bladder cancer, [169][170][171][172] lung cancer, 173 liver cancer 174 and even skin cancer. [175][176][177] The subject of theranostic platforms and other advances of magnetic NPs will be discussed in the following section of this paper.…”