Doxorubicin-Conjugated Iron Oxide Nanoparticles Synthesized by Laser Pyrolysis: In Vitro Study on Human Breast Cancer Cells

Lungu, Iulia Ioana; Nistorescu, Simona; Badea, Mădălina Andreea; Petre, Andreea-Mihaela; Udrea, Ana-Maria; Banici, Ana-Maria; Fleaca, C.; Andronescu, Ecaterina; Dinischiotu, Anca; Dumitrache, Florian; Staicu, Angela; Balaș, Mihaela

doi:10.3390/polym12122799

Cited by 18 publications

(7 citation statements)

References 45 publications

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“…Several constructions were studied for DOX loading. Among them are gold [45], carbonate [40], iron oxide nanoparticles [21,39,41,42,[47][48][49][50][51][52][53][54][55][56][57][58][59], metal-organic frameworks [60], and much more laborious and complex constructions [61][62][63][64]. However, MNPs in the published works demonstrate only a low noncovalent drug loading capacity (µg/mg, DOX/MNP): 11 [55,56], 22 [57], 50 [42,51,58], 91 [54], 110 [41], 120 [65,66], 131 [50], 300 [53].…”

Section: Introductionmentioning

confidence: 99%

High Drug Capacity Doxorubicin-Loaded Iron Oxide Nanocomposites for Cancer Therapy

Kovrigina

Chubarov

2022

Magnetochemistry

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Magnetic nanoparticles (MNPs) have great potential in the drug delivery area. Iron oxide (Fe3O4) MNPs have demonstrated a promising effect due to their ferrimagnetic properties, large surface area, stability, low cost, easy synthesis, and functionalization. Some coating procedures are required to improve stability, biocompatibility, and decrease toxicity for medical applications. Herein, the co-precipitation synthesis of iron oxide MNPs coated with four types of primary surfactants, polyethylene glycol 2000 (PEG 2000), oleic acid (OA), Tween 20 (Tw20), and Tween 80 (Tw80), were investigated. Dynamic light scattering (DLS), ζ-potential, and transmission electron microscopy (TEM) techniques were used for morphology, size, charge, and stability analysis. Methylene blue reactive oxygen species (ROS) detection assay and the toxicity experiment on the lung adenocarcinoma A549 cell line were conducted. Two loading conditions for anticancer drug doxorubicin (DOX) on MNPs were proposed. The first one provides high loading efficiency (~90%) with up to 870 μg/mg (DOX/MNPs) drug capacity. The second is perspective for extremely high capacity 1757 μg/mg with drug wasting (DOX loading efficiency ~24%). For the most perspective MNP_OA and MNP_OA_DOX in cell media, pH 7.4, 5, and 3, the stability experiments are also presented. MNP_OA_DOX shows DOX pH-dependent release in the acidic pH and effective inhibition of A549 cancer cell growth. The IC50 values were calculated as 1.13 ± 0.02 mM in terms of doxorubicin and 0.4 ± 0.03 µg/mL in terms of the amount of the nanoparticles. Considering this, the MNP_OA_DOX nano theranostics agent is a highly potential candidate for cancer treatment.

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Section: Introductionmentioning

confidence: 99%

High Drug Capacity Doxorubicin-Loaded Iron Oxide Nanocomposites for Cancer Therapy

Kovrigina

Chubarov

2022

Magnetochemistry

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“…The presence of these bands confirmed that the DOX successfully conjugated with AZnFe-NPs. 50 The bands at 3435 and 1601 cm −1 for the plain SA/CMC mixture (which was used as a placebo in this study) were attributed to stretching vibrations of –COOH and those of O–H and C=O, respectively. In the spectrum of SACMC-DOX, the C=O stretching frequency was shifted to 1597 cm −1 .…”

Section: Resultsmentioning

confidence: 81%

Composite Microgels Loaded with Doxorubicin-Conjugated Amine-Functionalized Zinc Ferrite Nanoparticles for Stimuli-Responsive Sustained Drug Release

Bellala,

Viswanathan,

Guntakanti

et al. 2024

IJN

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Purpose The purpose of this study is to address the need for efficient drug delivery with high drug encapsulation efficiency and sustained drug release. We aim to create nanoparticle-loaded microgels for potential applications in treatment development. Methods We adopted the process of ionic gelation to generate microgels from sodium alginate and carboxymethyl cellulose. These microgels were loaded with doxorubicin-conjugated amine-functionalized zinc ferrite nanoparticles (AZnFe-NPs). The systems were characterized using various techniques. Toxicity was evaluated in MCF-7 cells. In vitro release studies were conducted at different pH levels at 37 o C, with the drug release kinetics being analyzed using various models. Results The drug encapsulation efficiency of the created carriers was as high as 70%. The nanoparticle-loaded microgels exhibited pH-responsive behavior and sustained drug release. Drug release from them was mediated via a non-Fickian type of diffusion. Conclusion Given their high drug encapsulation efficiency, sustained drug release and pH-responsiveness, our nanoparticle-loaded microgels show promise as smart carriers for future treatment applications. Further development and research can significantly benefit the field of drug delivery and treatment development.

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“…This method allows users to quickly obtain product, and the yield was quite considerable as well. Furthermore, the method was easy and required no sophisticated instruments compared to other methods [ 30 ]. The HO@Fe 3 O 4 was then modified with an amine group and further polymerization of MAA and NIPAM through RAFT polymerization.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of Multi-Functional Nano-Vectors for Target-Specific Drug Delivery

Lee

Lai

2021

Polymers

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Magnetic nanoparticles have gained attention in cancer therapy due to their non-toxic properties and high bio-compatibility. In this report, we synthesize a dual-responsive magnetic nanoparticle (MNP) that is sensitive to subtle pH and temperature change as in the tumor microenvironment. Thus, the functional doxorubicin (DOX)-loaded MNP (DOX-PNIPAM-PMAA@Fe3O4) can perform specific DOX releases in the cancer cell. The particle was characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta-potential, Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). The microscopy data revealed the particle as having a spherical shape. The zeta-potential and size distribution analysis data demonstrated the difference for the stepwise modified MNPs. The FTIR spectrum showed characteristic absorption bands of NH2-SiO2@Fe3O4, CPDB@Fe3O4, PMAA@Fe3O4, and PNIPAM-PMAA@Fe3O4. Drug-loading capacity and releasing efficiency were evaluated under different conditions. Through an in vitro analysis, we confirmed that PNIPAM-PMAA@Fe3O4 has enhanced drug releasing efficiency under acidic and warmer conditions. Finally, cellular uptake and cell viability were estimated via different treatments in an MDA-MB-231 cell line. Through the above analysis, we concluded that the DOX-loaded particles can be internalized by cancer cells, and such a result is positive and prospective.

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Doxorubicin-Conjugated Iron Oxide Nanoparticles Synthesized by Laser Pyrolysis: In Vitro Study on Human Breast Cancer Cells

Cited by 18 publications

References 45 publications

High Drug Capacity Doxorubicin-Loaded Iron Oxide Nanocomposites for Cancer Therapy

High Drug Capacity Doxorubicin-Loaded Iron Oxide Nanocomposites for Cancer Therapy

Composite Microgels Loaded with Doxorubicin-Conjugated Amine-Functionalized Zinc Ferrite Nanoparticles for Stimuli-Responsive Sustained Drug Release

Synthesis of Multi-Functional Nano-Vectors for Target-Specific Drug Delivery

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