Downstream processing of virus-like particles: Single-stage and multi-stage aqueous two-phase extraction

Effio, Christopher Ladd; Wenger, Lukas; Ötes, Ozan; Oelmeier, Stefan A.; Kneusel, Richard E.; Hubbuch, Jürgen

doi:10.1016/j.chroma.2015.01.007

Cited by 64 publications

(39 citation statements)

References 51 publications

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“…This early-stage processing can then be integrated with further HIV-GFP VLP concentration and purification steps such as an ATPE multi-stage extraction [37], ATPE back extraction or other methodologies such as PEG precipitation [38] or anion exchange chromatography [39]. These results highlight ATPE in general as a promising technique to improve the effectiveness of VLP downstream processing, potentially providing a response to the increasing demand for high product titers.…”

Section: Discussionmentioning

confidence: 95%

Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant

Jacinto

Soares

Azevedo

et al. 2015

Separation and Purification Technology

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Section: Discussionmentioning

confidence: 95%

Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant

Jacinto

Soares

Azevedo

et al. 2015

Separation and Purification Technology

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“…ATPS was also investigated for the purification of VLPs (rotavirus-like particles), results showed a recovery of 85 % [112] and most recently single-stage and multi-stage ATPS has been employed for the purification of VLPs (human B19 parvo) from crude Spodoptera frugiperda Sf 9 cell lysate. First time, a multi-stage ATPE was shown for VLPs, with satisfying results [113]. …”

Section: Applications Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

Aqueous two-phase system (ATPS): an overview and advances in its applications

et al. 2016

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Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia. Although, the partition behavior involved in the method is complex and difficult to predict. Current research shows that it has also been successfully used in the detection of veterinary drug residues in food, separation of precious metals, sewage treatment and a variety of other purposes. The ATPS is able to give high recovery yield and is easily to scale up. It is also very economic and environment friendly method. The aim of this review is to overview the basics of ATPS, optimization and its applications.

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“…. When the systems are characterized, different partitioning studies for biomolecules can follow as shown for model proteins like lysozyme and pharmaceutically relevant molecules such as mAbs and virus‐like particles from human B19 parvo‐virus .…”

Section: Experimental Approachesmentioning

confidence: 99%

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

Baumann

Hubbuch

2016

Engineering in Life Sciences

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The biopharmaceutical industry is at a turning point moving toward a more customized and patient-oriented medicine (precision medicine). Straightforward routines such as the antibody platform process are extended to production processes for a new portfolio of molecules. As a consequence, individual and tailored productions require generic approaches for a fast and dedicated purification process development. In this article, different effective strategies in biopharmaceutical purification process development are reviewed that can analogously be used for the new generation of antibodies. Conventional approaches based on heuristics and high-throughput process development are discussed and compared to modern technologies such as multivariate calibration and mechanistic modeling tools. Such approaches constitute a good foundation for fast and effective process development for new products and processes, but their full potential becomes obvious in a correlated combination. Thus, different combinatorial approaches are presented, which might become future directions in the biopharmaceutical industry.

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Downstream processing of virus-like particles: Single-stage and multi-stage aqueous two-phase extraction

Cited by 64 publications

References 51 publications

Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant

Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant

Aqueous two-phase system (ATPS): an overview and advances in its applications

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

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