Downstream processing of mannosylerythritol lipids produced by Pseudozyma aphidis

Abstract

Downstream processing of mannosylerythritol lipids produced by Pseudozyma aphidisPseudozyma aphidis DSM 14930 was used for the bioreactor production of mannosylerythritol lipids (MEL). A scheme is presented for the isolation and purification of MEL by using different solvents. Adsorption experiments of MEL at Amberlite XAD resins were performed. Up to 93% (wt-%) MEL could be transferred from the culture suspension into a newly formed, highly viscous solid phase by heating the culture suspension to !100 7C. This phase contained on average 87% (wt-%) MEL and could be simply isolated by pouring off the supernatant, without producing solvent waste.

Mannosylerythritol lipids: a review

“…Solvent extraction, adsorption on diVerent types of Amberlite XAD followed by solvent extraction and heating the culture suspension to 110°C for 10 min were the three downstream methods reported. The last method gave the highest yield of 93 wt% with a purity of 87 wt%, whereas the solvent extraction method gave only 79 wt% yield with 100 wt% purity [56]. …”

“…Rau et al [56] reported the production of MEL in a bioreactor and the various downstream processing strategies for its recovery and puriWcation. Solvent extraction, adsorption on diVerent types of Amberlite XAD followed by solvent extraction and heating the culture suspension to 110°C for 10 min were the three downstream methods reported.…”

“…The compounds on the plates are located by charring them at 110°C for 5 min after spraying a mixture of anthrone and sulfuric acid [10,34,56]. The concentrated glycolipids are dissolved in chloroform and then puriWed by silica gel (Wako-gel C-200) column chromatography using a gradient elution technique with chloroformacetone (10:0-0:10, vol/vol) solvent mixtures [10,56]. Table 3 summarizes the production conditions and the corresponding yield in the preparation of MEL by Pseudozyma sp.…”

“…Seed cultures are prepared by inoculating cells grown on slants into test tubes containing a growth medium [4% glucose, 0.3% NaNO 3 , 0.03% MgSO 4 , 0.03% KH 2 PO 4 , 0.1% yeast extract (pH 6.0)] at 25°C on a reciprocal shaker (150 rpm) for 2 days [10,33,34,56]. Exactly 1 mL of the seed culture is transferred to a 300 mL Erlenmeyer Xask containing 30 mL of the basal medium [4% olive oil, 0.3% NaNO 3 , 0.03% MgSO 4 , 0.03% KH 2 PO 4 , 0.1% yeast extract (pH 6.0)], which is then incubated on a rotary shaker (220 rpm) at 28°C for 7 days.…”

Glycolipid biosurfactants: Potential related biomedical and biotechnological applications

“…Oliveira et al 25 Whang et al 26 Jadhav et al 27 Christova et al 28 Gudiña et al 29 Glucolipid 73 Hewald et al 74 Rau et al 75 Fukuoka et al 76 Morita et al 77 Morita et al 78 Mannosylribitol lipid and mannosylarabitol lipid…”