Downstream processing of hyperforin from Hypericum perforatum root cultures

Haas, Paul; Gaid, Mariam; Zarinwall, Ajmal; Beerhues, Ludger; Scholl, Stephan

doi:10.1016/j.ejpb.2017.06.026

Cited by 6 publications

(10 citation statements)

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“…The growth kinetic during the linear phase was expressed via the volumetric biomass formation rate r X (Figure 4). The maximum r X was observed within the linear phase (days [16][17][18][19][20][21][22] to be between 6.3-9.0 g L −1 day −1 (Figures 3A, 4A). Similar growth patterns were observed for H. perforatum and Echinacea purpurea root cultures [32,33].…”

Section: The Growth Characteristics Of Sjw Root Culturesmentioning

confidence: 94%

“…HPLC‐DAD analysis was carried out as described previously , except that the HPLC column used was a XBridge BEH C 18 column (3.5 µm, 4.6 × 100 mm). The hyperforin content was determined as described previously using authentic hyperforin as a reference to set a calibration curve.…”

Section: Methodsmentioning

confidence: 99%

“…Each STBR was supported with Rushton turbines (6-bladded impeller rotates at 50 min −1 ). The cultivation period was terminated at various time intervals (10,20,30, and 40 days, one STBR at a time). The dry and fresh weights were determined followed by determination of the hyperforin content as discussed in Sections 2.2 and 2.8, respectively.…”

Section: Bioreactor Cultivationmentioning

confidence: 99%

“…Interestingly, these in vitro roots overcome the traditional wild‐plant limitation of cost/efficacy ratio for production of extracts with high medicinal value. First, because they are devoid of hypericin, which diminishes the need of costly CO 2 ‐based supercritical extraction; second, they are free from chlorophyll, whose presence is known to interfere with the downstream processing . Recently, pure hyperforin has been proven to have anti‐proliferative and anti‐migratory effects in 2D and 3D artificial skin constructs of human dermal fibroblasts .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, pure hyperforin has been proven to have anti‐proliferative and anti‐migratory effects in 2D and 3D artificial skin constructs of human dermal fibroblasts . In this context, the downstream processing of the hyperforin‐rich petroleum ether extract and its use as a potential anti‐proliferative agent using human keratinocytes, fibroblasts as well artificial skin constructs were investigated in detail .…”

Section: Introductionmentioning

confidence: 99%

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Toward enhanced hyperforin production in St. John's wort root cultures

Gaid

Grosch

Möller

et al. 2019

Engineering in Life Sciences

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During the past decades, several trials targeted a stable, sustainable and economic production of St. John's wort (Hypericum perforatum) extract. The value of this extract stems from its use to treat depression and skin irritation due to its hyperforin content. Previously, hyperforin‐forming in vitro root cultures were established. Here, detailed growth and production kinetics have been analyzed over 40 days of cultivation. In the first 10 days, sucrose was completely hydrolyzed to glucose and fructose. The ammonium consumption supported the increase in the biomass and hyperforin production. When sucrose was replaced with glucose/fructose, the linear growth phase started 6 days earlier and resulted in a higher space‐time‐yield. The maximum hyperforin production was 0.82 mg L−1 day−1, which was 67 % higher than in the sucrose‐supplemented standard cultivation. Buffering the sucrose‐supplemented medium with phosphate caused a 2.7‐fold increase in the product to biomass yield coefficient. However, the combination of monosaccharides and buffering conditions did not cause an appreciable improvements in the production performance of the shake flask approaches. A potential scalability from flask to lab‐scale stirred bioreactors has been demonstrated. The results obtained offer a basis for a scalable production of hyperforin and a sustainable source for a tissue culture‐based phytomedicine.

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