Downregulatory effect of miR-342-3p on epileptogenesis in the PTZ-kindling model

Pala, Mukaddes; Meral, İsmail; Acikgoz, Nilgun Pala; Yılmaz, Şenay; Okur, Semra Karaca; Acar, Seyma; Polat, Yalcin; Akbaş, Fahri

doi:10.1007/s11033-022-08017-5

Cited by 2 publications

(2 citation statements)

References 49 publications

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“…In a recent study, it was shown that GRM2 is negatively regulated by miR-342- p , one of miRNA expressed in the epileptic hippocampus. Upon pharmacological induction of tonic–clonic seizures in rats, the downregulation of miR-342- p and upregulation of its target gene GRM2 were observed [ 79 ]. These results suggested a possible involvement of GMR2 in epileptogenesis.…”

Section: Discussionmentioning

confidence: 99%

Impact of the m.13513G>A Variant on the Functions of the OXPHOS System and Cell Retrograde Signaling

Kidere

Zayakin

Livcane

et al. 2023

CIMB

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Mitochondria are involved in many vital functions in living cells, including the synthesis of ATP by oxidative phosphorylation (OXPHOS) and regulation of nuclear gene expression through retrograde signaling. Leigh syndrome is a heterogeneous neurological disorder resulting from an isolated complex I deficiency that causes damage to mitochondrial energy production. The pathogenic mitochondrial DNA (mtDNA) variant m.13513G>A has been associated with Leigh syndrome. The present study investigated the effects of this mtDNA variant on the OXPHOS system and cell retrograde signaling. Transmitochondrial cytoplasmic hybrid (cybrid) cell lines harboring 50% and 70% of the m.13513G>A variant were generated and tested along with wild-type (WT) cells. The functionality of the OXPHOS system was evaluated by spectrophotometric assessment of enzyme activity and high-resolution respirometry. Nuclear gene expression was investigated by RNA sequencing and droplet digital PCR. Increasing levels of heteroplasmy were associated with reduced OXPHOS system complex I, IV, and I + III activities, and high-resolution respirometry also showed a complex I defect. Profound changes in transcription levels of nuclear genes were observed in the cell lines harboring the pathogenic mtDNA variant, indicating the physiological processes associated with defective mitochondria.

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Section: Discussionmentioning

confidence: 99%

Impact of the m.13513G>A Variant on the Functions of the OXPHOS System and Cell Retrograde Signaling

Kidere

Zayakin

Livcane

et al. 2023

CIMB

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“…The most important potential biomarkers are miRNAs, whose expression is associated with activity from ion channels. Among them are miR-30b [135], miR-219a [136], miR-335 [137], miR-342 [138], and miR-30a [25]. In epilepsy, the increased expression of miR-155 was observed in three independent studies [27][28][29].…”

Section: Other Potential Mirna-based Markersmentioning

confidence: 99%

MicroRNAs as Potential Biomarkers of Post-Traumatic Epileptogenesis: A Systematic Review

Vasilieva,

Timechko,

Lysova

et al. 2023

IJMS

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Structural or post-traumatic epilepsy often develops after brain tissue damage caused by traumatic brain injury, stroke, infectious diseases of the brain, etc. Most often, between the initiating event and epilepsy, there is a period without seizures—a latent period. At this time, the process of restructuring of neural networks begins, leading to the formation of epileptiform activity, called epileptogenesis. The prediction of the development of the epileptogenic process is currently an urgent and difficult task. MicroRNAs are inexpensive and minimally invasive biomarkers of biological and pathological processes. The aim of this study is to evaluate the predictive ability of microRNAs to detect the risk of epileptogenesis. In this study, we conducted a systematic search on the MDPI, PubMed, ScienceDirect, and Web of Science platforms. We analyzed publications that studied the aberrant expression of circulating microRNAs in epilepsy, traumatic brain injury, and ischemic stroke in order to search for microRNAs—potential biomarkers for predicting epileptogenesis. Thus, 31 manuscripts examining biomarkers of epilepsy, 19 manuscripts examining biomarkers of traumatic brain injury, and 48 manuscripts examining biomarkers of ischemic stroke based on circulating miRNAs were analyzed. Three miRNAs were studied: miR-21, miR-181a, and miR-155. The findings showed that miR-21 and miR-155 are associated with cell proliferation and apoptosis, and miR-181a is associated with protein modifications. These miRNAs are not strictly specific, but they are involved in processes that may be indirectly associated with epileptogenesis. Also, these microRNAs may be of interest when they are studied in a cohort with each other and with other microRNAs. To further study the microRNA-based biomarkers of epileptogenesis, many factors must be taken into account: the time of sampling, the type of biological fluid, and other nuances. Currently, there is a need for more in-depth and prolonged studies of epileptogenesis.

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Downregulatory effect of miR-342-3p on epileptogenesis in the PTZ-kindling model

Cited by 2 publications

References 49 publications

Impact of the m.13513G>A Variant on the Functions of the OXPHOS System and Cell Retrograde Signaling

Impact of the m.13513G>A Variant on the Functions of the OXPHOS System and Cell Retrograde Signaling

MicroRNAs as Potential Biomarkers of Post-Traumatic Epileptogenesis: A Systematic Review

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